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| 2. |
- Dorafshan, Eshagh, et al.
(författare)
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Hierarchical recruitment of Polycomb complexes revisited
- 2017
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Ingår i: Nucleus. - : Taylor & Francis Group. - 1949-1034 .- 1949-1042. ; 8:5, s. 496-505
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Tidskriftsartikel (refereegranskat)abstract
- Polycomb Group (PcG) proteins epigenetically repress key developmental genes and thereby control alternative cell fates. PcG proteins act as complexes that can modify histones and these histone modifications play a role in transmitting the memory of the repressed state as cells divide. Here we consider mainstream models that link histone modifications to hierarchical recruitment of PcG complexes and compare them to results of a direct test of interdependence between PcG complexes for recruitment to Drosophila genes. The direct test indicates that PcG complexes do not rely on histone modifications to recognize their target genes but use them to stabilize the interactions within large chromatin domains. It also shows that multiple strategies are used to coordinate the targeting of PcG complexes to different genes, which may make the repression of these genes more or less robust.
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| 3. |
- Keuenhof, Katharina, 1994, et al.
(författare)
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Nuclear envelope budding and its cellular functions
- 2023
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Ingår i: Nucleus. - : Informa UK Limited. - 1949-1034 .- 1949-1042. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- The nuclear pore complex (NPC) has long been assumed to be the sole route across the nuclear envelope, and under normal homeostatic conditions it is indeed the main mechanism of nucleo-cytoplasmic transport. However, it has also been known that e.g. herpesviruses cross the nuclear envelope utilizing a pathway entitled nuclear egress or envelopment/de-envelopment. Despite this, a thread of observations suggests that mechanisms similar to viral egress may be transiently used also in healthy cells. It has since been proposed that mechanisms like nuclear envelope budding (NEB) can facilitate the transport of RNA granules, aggregated proteins, inner nuclear membrane proteins, and mis-assembled NPCs. Herein, we will summarize the known roles of NEB as a physiological and intrinsic cellular feature and highlight the many unanswered questions surrounding these intriguing nuclear events.
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| 4. |
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| 5. |
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| 6. |
- Percipalle, P
(författare)
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Co-transcriptional nuclear actin dynamics
- 2013
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Ingår i: Nucleus (Austin, Tex.). - : Informa UK Limited. - 1949-1042 .- 1949-1034. ; 4:1, s. 43-52
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Tidskriftsartikel (refereegranskat)
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| 7. |
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| 9. |
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| 10. |
- Söderberg, Emilia, et al.
(författare)
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Profilin is associated with transcriptionally active genes
- 2012
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Ingår i: Nucleus. - : Informa UK Limited. - 1949-1034 .- 1949-1042. ; 3:3, s. 290-299
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Tidskriftsartikel (refereegranskat)abstract
- We have raised antibodies against the profilin of Chironomus tentans to study the location of profilin relative to chromatin and to active genes in salivary gland polytene chromosomes. We show that a fraction of profilin is located in the nucleus, where profilin is highly concentrated in the nucleoplasm and at the nuclear periphery. Moreover, profilin is associated with multiple bands in the polytene chromosomes. By staining salivary glands with propidium iodide, we show that profilin does not co-localize with dense chromatin. profilin associates instead with protein-coding genes that are transcriptionally active, as revealed by co-localization with hnRNP and snRNP proteins. We have performed experiments of transcription inhibition with actinomycin D and we show that the association of profilin with the chromosomes requires ongoing transcription. however, the interaction of profilin with the gene loci does not depend on RNA. Our results are compatible with profilin regulating actin polymerization in the cell nucleus. however, the association of actin with the polytene chromosomes of C. tentans is sensitive to RNase, whereas the association of profilin is not, and we propose therefore that the chromosomal location of profilin is independent of actin.
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