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- Hyde, Kevin D., et al.
(författare)
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Incorporating molecular data in fungal systematics: a guide for aspiring researchers
- 2013
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Ingår i: Current Research in Environmental and Applied Mycology. - : Mushroom Research Foundation. - 2229-2225. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- The last twenty years have witnessed molecular data emerge as a primary research instrument in most branches of mycology. Fungal systematics, taxonomy, and ecology have all seen tremendous progress and have undergone rapid, far-reaching changes as disciplines in the wake of continual improvement in DNA sequencing technology. A taxonomic study that draws from molecular data involves a long series of steps, ranging from taxon sampling through the various laboratory procedures and data analysis to the publication process. All steps are important and influence the results and the way they are perceived by the scientific community. The present paper provides a reflective overview of all major steps in such a project with the purpose to assist research students about to begin their first study using DNA-based methods. We also take the opportunity to discuss the role of taxonomy in biology and the life sciences in general in the light of molecular data. While the best way to learn molecular methods is to work side by side with someone experienced, we hope that the present paper will serve to lower the learning threshold for the reader.
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| 2. |
- Krupodorova, TA, et al.
(författare)
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Review of the basic cultivation conditions influence on the growth of basidiomycetes
- 2021
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Ingår i: Current Research in Environmental and Applied Mycology (Journal of Fungal Biology). - : Institute of Plant and Environment Protection (IPEP). - 2229-2225. ; 11:1, s. 494-531
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Forskningsöversikt (refereegranskat)abstract
- Recently, the significant economic and therapeutic potential of different fungi causes the intense cultivation of the prospective species. This review presents the analysis of the basic physicochemical conditions for fungi cultivation that have an influence on basidiomycetes’ mycelia or biomass growth options (maximal mycelial dry weight, maximal mycelial growth, maximal mycelial growth rate, mycelial density, colony diameter, etc.). Not only different species, but different strains of the same fungus require different cultivation conditions, the variability of nutrition media, temperature, pH, carbon and nitrogen sources. Several parameters optimal cultivation can be explained by the geographical and climatic factors. Analysis of literature data and the resulting conclusions will help a deeper understanding of the biological characteristics of fungi, their nutritional needs, optimal temperature and pH, and optimization of the production of fungal biomass. Comparison of the collected information on various fungal strains growth aimed to facilitate the further development and optimization in existing cultivation protocols. According to this, the maximum growth for biotechnological and industrial application is expected to be obtained. Also, the prolongation in safekeeping of viable fungal forms and the preservation of the fungi biodiversity for their re-introduction in nature is expected as one of the benefits after protocol optimization.
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