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- Ghorbani, Abdolbaset, et al.
(författare)
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Molecular Authentication of Radix Behen Albi ("Bahman Sefid") Commercial Products Reveals Widespread Adulteration
- 2020
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Ingår i: Research Journal of Pharmacognosy. - : The Iranian Society of Pharmacognosy. - 2345-4458 .- 2345-5977. ; 7:4, s. 57-64
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Tidskriftsartikel (refereegranskat)abstract
- Background and objectives: The roots of Centaurea behen L., (Asteraceae) known as Radix Behen Albi are used as an aphrodisiac, anti-lithiasis and general tonic. It is available as dried or powdered roots in the herbal markets of Iran. Confirming the identity of this medicinal root using conventional methods is challenging because of lack of the diagnostic characters and market samples are easy to misidentify or adulterate.Methods: This study aimed to authenticate 13 Radix Behen Albisamples purchased from different herbal markets in Iran and to identify the potential adulterants through DNA barcoding. Nuclear (nrITS) and plastid (trnL-F spacer, matK and rbcL) DNA regions were used as barcoding markers. A reference database was compiled using sequences from herbarium voucher specimens and publicly available sequences.Results: Among used barcode regions nrITS was the best marker for species identification followed by trnL-F spacer. MatK and rbcL were able to identify samples to the family level. This study showed that none of the market samples belonged to the authentic Centaurea behen L. Sixty-nine percent of samples were Cousinia spp. (Asteraceae), 23% Korshinskya spp. (Apiaceae) and 8% Crambe spp. (Brassicaceae). This substitution does not only hinder consumers obtain the desired medicinal effects of Radix Behen Albi but also raises concerns about the pharmacovigilance of this medicinal root sold in the markets.Conclusion: The present study shows the need for monitoring and authentication of crude herbal drugs in the markets of Iran, and that DNA barcoding is a suitable tool for this purpose.
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| 2. |
- Farida, Seyed Hamed Moazzami, et al.
(författare)
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Ethnobotanical Applications and Their Correspondence with Phylogeny in Apiaceae-Apioideae
- 2018
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Ingår i: RESEARCH JOURNAL OF PHARMACOGNOSY. - 2345-5977. ; 5:3, s. 79-97
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Forskningsöversikt (refereegranskat)abstract
- The Apiaceae family includes some of the most known medicinal, food and spice species in the world some of which have been used by humans since antiquity. Local people in different regions use many species of the family but the information regarding their uses is scattered. Traditionally used species are good candidates for bioprospecting. Combining traditional uses with phylogenetic data helps in selecting species for bioprospecting. In the present study, an ethnobotanical literature review was conducted to outline a comprehensive overview of the ethnobotanical importance of the family in Iran. To highlight the most ethnobotanically used groups in the family, ethnobotanical data were overlapped with generic phylogeny tree of the subfamily Apioideae. In total, 72 species (17.27%) from 42 genera (33.87%) were found to have ethnobotanical uses. Main uses of the family members were medicinal purposes (67.30%) followed by culinary (25%) and ethnoveterinary (11.11%) uses. Two categories of potential species groups for further bioprospecting investigations have been identified based on the phylogenetic analysis. Based on the findings of the present study, phylogenetic analysis can help in identifying potential groups for bioprospecting studies. More rigorous phylogenetic analysis that combines chemotaxonomy data, ethnomedicinal data and phylogenetic data are necessary to pinpoint exact lineages for specific medicinal properties.
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