| 1. |
- Andreeva, HD, et al.
(author)
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Kinetic of monocytes Fc gamma receptors in patients after coronary artery bypass
- 2011
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In: CENTRAL EUROPEAN JOURNAL OF MEDICINE. - : Walter de Gruyter GmbH. - 1895-1058. ; 6:1, s. 31-36
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Journal article (other academic/artistic)abstract
- Cardiopulmonary bypass is usually associated with an increased risk of post-operative infections and systemic inflammatory response syndrome. This is accompanied by a neutrophil leucocytosis and lymphopenia. Less is known about the role of monocytes and markers of monocyte activity. This study focuses on the changes of Fc-gamma receptors on monocytes in patients undergoing on-pump coronary artery bypass grafting (CABG).The surface expression of CD64, CD32 and CD16 were studied using flow cytometry in 37 patients scheduled for CABG. The antigen density and the percentage positive cells were monitored preoperatively and on day 1, 2, 3 and 7 postoperatively. CD64 and CD32 antigen density were significantly increased from day 1 to day 7(p<0.0001). A significant increase (p<0.0001) in the percentage CD16+ monocytes was detected at day 1,2,and 3. The downregulation of CD16 expression on day 1 was followed be elevation at days 2,3(p<0.01). On day 7th percentage CD16+ monocytes and density were not returned to baseline values. Only the baseline levels of CD64 was lower compared to controls(p<0.05). The results suggest that on-pump CABG induces dynamic changes in the expression of Fc-gamma receptors on monocytes as late as 7 days. We observed significant upregulation in the expression of CD64 and CD32 and “to phases” distribution of CD16 in the post-CABG period.
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| 2. |
- Diaz Cruz, Maria Araceli, et al.
(author)
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Cis-regulatory elements in conserved non-coding sequences of nuclear receptor genes indicate for crosstalk between endocrine systems
- 2021
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In: Open Medicine (Poland). - : De Gruyter Open. - 2391-5463. ; 16:1, s. 640-650
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Journal article (peer-reviewed)abstract
- Nuclear receptors (NRs) are ligand-activated transcription factors that regulate gene expression when bound to specific DNA sequences. Crosstalk between steroid NR systems has been studied for understanding the development of hormone-driven cancers but not to an extent at a genetic level. This study aimed to investigate crosstalk between steroid NRs in conserved intron and exon sequences, with a focus on steroid NRs involved in prostate cancer etiology. For this purpose, we evaluated conserved intron and exon sequences among all 49 members of the NR Superfamily (NRS) and their relevance as regulatory sequences and NR-binding sequences. Sequence conservation was found to be higher in the first intron (35%), when compared with downstream introns. Seventy-nine percent of the conserved regions in the NRS contained putative transcription factor binding sites (TFBS) and a large fraction of these sequences contained splicing sites (SS). Analysis of transcription factors binding to putative intronic and exonic TFBS revealed that 5 and 16%, respectively, were NRs. The present study suggests crosstalk between steroid NRs, e.g., vitamin D, estrogen, progesterone, and retinoic acid endocrine systems, through cis-regulatory elements in conserved sequences of introns and exons. This investigation gives evidence for crosstalk between steroid hormones and contributes to novel targets for steroid NR regulation.
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| 3. |
- Ghosal, Anubrata, et al.
(author)
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RNA interference and its therapeutic potential
- 2011
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In: Central European Journal of Medicine. - : Versita. - 1895-1058 .- 1644-3640. ; 6:2, s. 137-147
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Research review (peer-reviewed)abstract
- RNA interference is a technique that has become popular in the past few years. This is a biological method to detect the activity of a specific gene within a cell. RNAi is the introduction of homologous double stranded RNA to specifically target a gene’s product resulting in null or hypomorphic phenotypes. This technique involves the degradation of specific mRNA by using small interfering RNA. Both microRNA (miRNA) and small interfering RNA (siRNA) are directly related to RNA interference. RNAi mechanism is being explored as a new technique for suppressing gene expression. It is an important issue in the treatment of various diseases. This review considers different aspects of RNAi technique including its history of discovery, molecular mechanism, gene expression study, advantages of this technique against previously used techniques, barrier associated with this technique, and its therapeutic application.
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| 4. |
- Hooshmand, Arash
(author)
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Accurate diagnosis of prostate cancer using logistic regression
- 2021
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In: Open Medicine (Poland). - : Walter de Gruyter GmbH. - 2391-5463. ; 16:1, s. 459-463
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Journal article (peer-reviewed)abstract
- A new logistic regression-based method to distinguish between cancerous and noncancerous RNA genomic data is developed and tested with 100% precision on 595 healthy and cancerous prostate samples. A logistic regression system is developed and trained using whole-exome sequencing data at a high-level, i.e., normalized quantification of RNAs obtained from 495 prostate cancer samples from The Cancer Genome Atlas and 100 healthy samples from the Genotype-Tissue Expression project. We could show that both sensitivity and specificity of the method in the classification of cancerous and noncancerous cells are perfectly 100%.
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| 5. |
- Hortobágyi, Tibor, et al.
(author)
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Pathophysiology of meningioma growth in pregnancy
- 2017
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In: Open Medicine (Poland). - : Walter de Gruyter GmbH. - 2391-5463. ; 12:1, s. 195-200
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Research review (peer-reviewed)abstract
- Meningioma is among the most frequent brain tumours predominantly affecting elderly women. Epidemiological studies have shown that at the age of fertility the incidence is relatively low. The biological behaviour of meningioma in pregnancy is different from other meningiomas. The possible explanation is rooted in the complex physiological changes and hormonal differences during pregnancy. The increased meningioma growth observed in pregnancy is presumably the result of endocrine mechanisms. These include increase in progesterone, human placental lactogen (hPL) and prolactin (PRL) serum levels. In contrast, levels of pituitary hormones such as follicle stimulating hormone (FSH), luteinizing hormone (LH) and human chorionic gonadotropin (hCG) produced by the placenta are decreasing in the mother prior to childbirth. Besides, vascular factors also play a crucial role. Peritumoral brain edema (PTBE), with well-known causative association with vascular endothelial growth factor (VEGF), can often be seen both with imaging and in the surgical specimens. Our aim is to assess published research on this topic including diagnostic and therapeutic guidelines, and to provide a clinically useful overview on the pathophysiology and biological behaviour of this rare complication of pregnancy.
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| 6. |
- Jaruseviciene, Lina, et al.
(author)
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A study of antibiotic prescribing: the experience of Lithuanian and Russian GPs
- 2012
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In: Central European Journal of Medicine. - : Walter de Gruyter GmbH. - 1644-3640. ; 7:6, s. 790-799
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Journal article (peer-reviewed)abstract
- Background. Globally, general practitioners (GPs) write more than 90% of all antibiotic prescriptions. This study examines the experiences of Lithuanian and Russian GPs in antibiotic prescription for upper respiratory tract infections, including their perceptions of when it is not indicated clinically or pharmacologically. Methods. 22 Lithuanian and 29 Russian GPs participated in five focus group discussions. Thematic analysis was used to analyse the data. Results. We identified four main thematic categories: patients' faith in antibiotics as medication for upper respiratory tract infections; patient potential to influence a GP's decision to prescribe antibiotics for upper respiratory tract infections; impediments perceived by GPs in advocating clinically grounded antibiotic prescribing with their patients, and strategies applied in physician-patient negotiation about antibiotic prescribing for upper respiratory tract infections. Conclusions. Understanding the nature of physician-patient interaction is critical to the effective pursuit of clinically grounded antibiotic use as this study undertaken in Lithuania and the Russian Federation has shown. Both physicians and patients must be targeted to ensure correct antibiotic use. Further, GPs should be supported in enhancing their communication skills about antibiotic use with their patients and encouraged to implement a shared decision-making model in their practices.
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| 7. |
- Machaczka, M, et al.
(author)
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Eosinophilia as a presenting symptom of the metastatic lung adenocarcinoma with an unknown primary localization
- 2011
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In: CENTRAL EUROPEAN JOURNAL OF MEDICINE. - : Walter de Gruyter GmbH. - 1895-1058. ; 6:5, s. 541-544
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Journal article (other academic/artistic)abstract
- Apparent hematological symptoms rarely dominate the clinical picture of an underlying non-hematological malignancy. Malignancy-associated eosinophilia can result from clonal or non-clonal proliferation of eosinophils. Here, we report the case of a 59-year-old man with metastatic adenocarcinoma of the lung with an unknown primary tumor site, which presented as hypereosinophilia, anemia, lymphadenopathy, weight loss, and malaise. Bone marrow biopsy disclosed metastatic adenocarcinoma positive in immunohistochemistry for cytokeratin 7. Further assessment of specimens obtained from the bronchoalveolar lavage and biopsy of the mediastinal lymph nodes confirmed the diagnosis of the metastatic lung cancer, although the primary tumor site remained undiscovered. This case underlines that eosinophilia may represent a rare primary manifestation of an undetected malignancy, and it is thus important to consider this as part of the differential diagnosis in patients presenting with unexplained eosinophilia.
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| 8. |
- Malinauskiene, Laura, et al.
(author)
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Formaldehyde may be found in cosmetic products even when unlabelled
- 2015
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In: Open Medicine (Poland). - : Walter de Gruyter GmbH. - 2391-5463. ; 10:1, s. 323-328
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Journal article (peer-reviewed)abstract
- Concomitant contact allergy to formaldehyde and formaldehyde-releasers remains common among patients with allergic contact dermatitis. Concentration of free formaldehyde in cosmetic products within allowed limits have been shown to induce dermatitis from shortterm use on normal skin. The aim of this study was to investigate the formaldehyde content of cosmetic products made in Lithuania. 42 samples were analysed with the chromotropic acid (CA) method for semi-quantitative formaldehyde determination. These included 24 leave-on (e.g., creams, lotions) and 18 rinse-off (e.g., shampoos, soaps) products. Formaldehyde releasers were declared on the labels of 10 products. No formaldehyde releaser was declared on the label of the only face cream investigated, but levels of free formaldehyde with the CA method was >40 mg/ml and when analysed with a high-performance liquid chromatographic method - 532 ppm. According to the EU Cosmetic directive, if the concentration of formaldehyde is above 0.05% a cosmetic product must be labelled "contains formaldehyde". It could be difficult for patients allergic to formaldehyde to avoid contact with products containing it as its presence cannot be determined from the ingredient labelling with certainty. The CA method is a simple and reliable method for detecting formaldehyde presence in cosmetic products.
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| 9. |
- Mogilevkina, Iryna, et al.
(author)
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Some reproductive and sexual health indicators in rape victims in Ukraine
- 2006
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In: Central European Journal of Medicine. - : Walter de Gruyter GmbH. ; 1:4, s. 399-415
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Journal article (peer-reviewed)abstract
- The objective was to study the demographics and some reproductive health indicators in Ukrainian women who reported having been raped. On the basis of responses to a questionnaire, we evaluated factors associated with a history of rape by a case-referent approach, using women reporting rape (n=177) as the dependent variable in comparison to those without such an experience (n=1444). Women reporting rape were more often younger than those without a history of rape. There was an association between a history of rape and three or more induced abortions. Rape victims were more likely to have a history of sexually transmitted infections and pelvic inflammatory diseases. A history of rape was more often associated with smoking and previous drug use. Women who had experienced rape more often reported more than one sexual partner during the last six months as well as during the previous year. On the other hand, these women reported sexual intercourse less frequently than did those without a history of rape. Rape victims more often reported sexual harassment at where they work or socialize as well as sexual abuse in childhood or within their family. Thus, a history of rape was common and associated with several negative health indicators. Sexual violence needs to be acknowledged in all parts of the Ukrainian society as a serious threat that requires urgent attention for the improvement of women’s reproductive and general health and human rights.
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| 10. |
- Stankunas, M, et al.
(author)
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Differences in reporting somatic complaints in elderly by education level
- 2013
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In: Central European Journal of Medicine. - : Walter de Gruyter GmbH. - 1895-1058 .- 1644-3640. ; 8:1, s. 125-131
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Journal article (peer-reviewed)abstract
- Aim: To evaluate the association between somatic complaints and education level among elders in selected European countries. Methods: Cross-sectional study among randomly selected community dwelling persons aged 60–84 years from seven cities in Europe: Ancona (Italy), Athens (Greece), Granada (Spain), Kaunas (Lithuania), Stuttgart (Germany), Porto (Portugal) and Stockholm (Sweden). Somatic complaints were measured with the 24-item version of the Giessen Complaint List (GBB-24). A regression analysis was done to investigate the association between education and somatic complaints. Results. The mean GBB-24 scale was 16.3±14.9. The most common complaints were pain in joints and limbs (29.6%), back-pain (24.1%), heaviness or tiredness in the legs (19.1%) and general tiredness (15.7%). Respondents with less than primary education reported higher levels of somatic symptoms compared to those with university degree. A regression analysis showed that higher education (OR=0.69) and being male (OR=0.48) were associated with a decreased risk for high levels of somatic complaints, and the opposite for older age (OR=1.03) and being single/divorced/window (OR=1.28). Conclusions: The results indicate that less educated elders are more likely to expressed somatic complaints.
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