| 1. |
- Banerjee, Indradumna, et al.
(författare)
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MicroCap : Microfluidic centrifuge assisted precipitation for DNA quantification on lab-on-DVD
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - : Chemical and Biological Microsystems Society. - 9781510897571 ; , s. 1802-1805
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Konferensbidrag (refereegranskat)abstract
- We report for the first time the MicroCAP technique, for rapid DNA detection and quantification, that does not require any purification or fluorescent labelling of DNA. The invention is based on DNA interacting with a detection dye (Gelred) to form a complex, that forms a visible precipitate within seconds of centrifugation. MicroCAP can be used for DNA quantification, when combined with the Lab-on-DVD with inbuilt centrifugation and sub-micron imaging resolution. We quantify PCR and LAMP assay products using MicroCAP on the integrated Lab-on-DVD platform, and demonstrate a detection limit of 10 ng/μl. Copyright
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| 2. |
- Banerjee, Indradumna, et al.
(författare)
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Particle focusing dynamics in extended elasto inertial flow
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - : Chemical and Biological Microsystems Society. - 9781510897571 ; , s. 472-475
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Konferensbidrag (refereegranskat)abstract
- We report the decoupled effects of inertial and viscous forces on particle focusing, the stability of particles, and particle trajectories to reach equilibrium position in an extended elasto inertial pressure driven flow, in a circular micro-capillary. We report numerically and experimentally for the first time, the existence of multiple stable equilibrium positions in the EEI regime, which was unobserved for flows previously studied at lower Reynolds number viscoelastic flows.
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| 3. |
- Beech, Jason P., et al.
(författare)
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Tunable separation and DNA manipulation in metal coated pillar arrays
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - 9781510897571 ; 4, s. 2090-2093
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Konferensbidrag (refereegranskat)abstract
- Implementing electrically connected metal-coated posts in a Deterministic Lateral Displacement (DLD) device and applying electric fields, we use electrokinetics to achieve tunable particle separations and to trap and manipulate DNA. The strength of DLD stems from its typically binary behavior. Particles move in one out of two trajectories based on their effective size. For particles that are close to the threshold size, a minute external force is sufficient to nudge the particles from one trajectory to another. Dielectrophoresis (DEP) provides such a force and also gives specificity based on the dielectric properties of the particles.
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| 4. |
- Core, Giulia, et al.
(författare)
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Method for selecting optimal operation frequencies in bulk acoustophoretic devices
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - 9781510897571 ; 1, s. 282-284
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Konferensbidrag (refereegranskat)abstract
- We report a new method to select the optimal actuation frequencies in bulk acoustophoresis devices, by means of differential impedance spectrum analysis measured via the actuating piezoelectric transducer (piezo). The impedance spectra is rich of large spurious resonance peaks originating from bulk resonances not related to the channel resonance, Fig. 1a, why direct measurement of the piezo impedance spectra is not a viable strategy. We present here for the first time that the resonance modes of the acoustophoresis channel can be clearly identified by sequentially measuring the impedance spectra of the acoustophoresis manifold when the channel is filled with two different fluids and calculate the normalised differential impedance spectra.
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| 5. |
- Hauser, Janosch, et al.
(författare)
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An autonomous blood microsampling device enabling metered large-volume dried plasma spots (DPS)
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - : Chemical and Biological Microsystems Society. - 9781510897571 ; , s. 1737-1739
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Konferensbidrag (refereegranskat)abstract
- This work introduces a novel principle for volume metering of passively separated blood plasma, empowered by an air pinch-off structure and the high capillary force of a paper matrix. The presented microfluidic device enables the autonomous generation of large-volume dried plasma spots (DPS) with 16 µl of plasma from 50-100 µl of human whole blood within less than 10 minutes. Providing large-volume DPS increases the confidence level of detecting low concentration analytes and constitutes a step towards using blood microsampling in the everyday blood analysis routine.
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| 6. |
- Lapins, Noa, et al.
(författare)
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Mobile-labdisc for point-of-care diagnostics at resource limited settings
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - : Chemical and Biological Microsystems Society. - 9781510897571 ; , s. 1762-1764
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Konferensbidrag (refereegranskat)abstract
- Anyone carrying a smartphone has the potential access to an electrical power bank capable of spinning a lab-disc, a sensor for imaging and the processing power to analyze data. Here, exploit the electrical power stored in smartphones to introduce a smartphone operated centrifugal platform made of cardboard and a small motor to provide a low-cost, portable, sample-to-answer centrifugal diagnostic systems, specifically designed to meet the needs in resource-limited-settings. As a proof of principle, sandwich ELISA immunoassay for detection of interleukin-2 and hematocrit level measurement are demonstrated.
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| 7. |
- Lenshof, Andreas, et al.
(författare)
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Measuring cancer cell compressibility by acoustophoresis separation experiments
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - 9781510897571 ; 1, s. 432-434
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Konferensbidrag (refereegranskat)abstract
- We present a new experimental method for the measurement of the compressibility of cells by analysis of free flow acoustophoresis separation data.
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| 8. |
- Mallinson, Jay, et al.
(författare)
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ACouWash : A standalone instrument for the washing, separation and enrichment of cells.
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - 9781510897571 ; 1, s. 279-281
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Konferensbidrag (refereegranskat)abstract
- We developed the AcouWash (Figures 1 and 2), a benchtop instrument for flow-through cell and particle separation by acoustophoresis. Building on previous research [1-4] AcouWash can re-suspend cells in new medium, separate cells based on size or acoustic properties, or enrich cells, Figure 3. We present our initial evaluation of AcouWash regarding size-based separation of particles, cell washing, cell viability, and time savings compared to centrifugation. We repeatedly recovered 90-100% of target cells and particles, washed away 99.9% of a small fluorescent tracer molecule, viability of cells was unchanged, and reduced the time for standard cell labelling by 50%.
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| 9. |
- Ohlander, Anna, et al.
(författare)
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Staphylococcus aureus sub-typing and detection of MRSA on a microfluidic lab-on-foil device
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - : Chemical and Biological Microsystems Society. - 9781510897571 ; , s. 1835-1838
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Konferensbidrag (refereegranskat)abstract
- Emerging antibiotic multi-resistance, increased travel and trade, and environmental changes have contributed to the prevalence of infectious diseases and rapid changes in the efficiency of therapeutics. We report detection of multi-resistant Staphylococus aureus and S. aureus strain sub-types using a microfluidic detection module with integrated thin film heater and DNA microarray, which enables surface-bound melting curve analysis. Successful discrimination between clinical S. aureus strain sub-types and between methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) was demonstrated. The microfluidic detection module is constructed completely on a plastic foil using roll-to-roll compatible fabrication methods that yield a very low-cost system. Copyright
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| 10. |
- Ström, Oskar E., et al.
(författare)
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Integrated on-chip system for DNA purification, labelling and surface stretching
- 2018
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - 9781510897571 ; 2, s. 1177-1180
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Konferensbidrag (refereegranskat)abstract
- We report a simplified, integrated and more efficient way of preparing DNA for analysis. We have developed a microfluidic platform that can purify DNA and subsequently label and stretch it on a surface. We have successfully extracted DNA from a gel-filled microfluidic channel using electrophoresis and subsequently stretched the DNA linearly by absorption to either a hydrophobic or a positively-charged surface. To our knowledge, DNA has not previously been stretched on to a surface while being moved using an electric field in this way. We have also labelled DNA after stretching with intercalating dyes. The intention, following stretching and labelling, is to analyze the DNA with optical mapping.
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