| 1. |
- Ainla, Alar, 1982, et al.
(författare)
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A multi-purpose microfluidic pipette for single-cell analysis
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010. - 9781618390622 ; 2, s. 932-934
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Konferensbidrag (refereegranskat)abstract
- We report a multi-purpose microfluidic pipette, with a recirculating liquid tip. This device, made in poly(dimethylsiloxane), enables contamination-free manipulation and chemical stimulation of selected single cells in cell collectives or tissue slices. The pipette is capable of carrying out a variety of complex fluid processing functionalities, such as mixing, multiplexing, or gradient generation. The concept is flexible and scalable as the geometry and the size of the recirculation zone is defined by pressure, channel number, and geometry. We have applied the pipette in a fluorescence uptake assay, electrophysiology studies and for chemical induction of membrane protrusion from biological cells.
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| 2. |
- Ardabili, Sahar, et al.
(författare)
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Dean flow-coupled inertial focusing for ultra-high-throughput particle filtration
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; , s. 1586-1588
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Konferensbidrag (refereegranskat)abstract
- Particle manipulation represents an important and fundamental step prior to counting, sorting and detecting bio-particles. In this study, we report dean-coupled inertial focusing of particles in flows through a single curve microchannel at extremely high channel Reynold numbers (∼325). We found the lateral particle focusing position, xf to be fixed and largely independent of radius of curvature and whether particles are pre-focused (at equilibrium) entering the curvature or randomly distributed. Finally, using a single inlet, u-shaped, microchannel we demonstrate filtration of 10μm particles from 2 μm particles at throughputs several orders of magnitude higher than previously shown.
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| 3. |
- Augustsson, P., et al.
(författare)
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Extraction of circulating tumor cells from blood using acoustophoresis
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; 3, s. 1592-1594
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Konferensbidrag (refereegranskat)abstract
- We present, for the first time, separation of three different prostate cancer cell lines from leukocyte fractions by means of continuous flow acoustophoresis. This flow-through separation approach, which utilize acoustophoretic forces to extract CTCs from blood has a significant advantage over affinity based methods in the sense that it comprises a non-contact and label free separation of CTCs from blood.
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| 4. |
- Basabe-Desmonts, L., et al.
(författare)
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Disposable bioanalytical microdevice for monitoring the effect of anti-platelet drugs
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - : CBMS. - 9781618390622 ; , s. 1388-1390
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Konferensbidrag (refereegranskat)abstract
- We report a disposable self-powered integrated microfluidic chip that enables a rapid and simple platelet-function assay from small samples of whole blood. The chip integrates a single-cell adhesion assay with a microfluidic platform; it enables accurate quantification of platelet adhesion, and it controls whole blood flow rate, shear stress, volume of sample, and assay time.
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| 5. |
- Beech, Jason P., et al.
(författare)
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Morphology-based sorting-blood cells and parasites
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; 2, s. 1343-1345
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Konferensbidrag (refereegranskat)abstract
- Morphology represents a hitherto unexploited source of specificity in microfluidic particle separation and may serve as the basis for label-free particle fractionation. There is a wealth of morphological changes in blood cells due to a wide range of clinical conditions, diseases, medication and other factors. Also, blood-borne parasites differ in morphology from blood cells. We present the use of Deterministic Lateral Displacement to create a chip-based, label-free diagnostic tool, capable of harvesting some of the wealth of information locked away in red blood cell morphology. We also use the device to separate the parasites that cause sleeping sickness from blood.
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| 6. |
- Carlborg, Carl Fredrik, 1981-, et al.
(författare)
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BEYOND PDMS: : OFF-STOCHIOMETRY THIOL-ENE BASED SOFT LITHOGRAPHY FOR RAPID PROTOTYPING OF MICROFLUIDIC DEVICES
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (micro TAS 2010). - 9781618390622 ; , s. 70-72
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Konferensbidrag (refereegranskat)abstract
- We present an easy to use, rapid fabrication platform for microfluidic systems, based on micro-molding of novel thiolene based polymer formulations. The novel fabrication platform addresses major drawbacks of PDMS by allowing large freedom in material and surface properties, including: (photo)patterning of stable surface modifications, bonding without plasma treatment, rapid UV or thermal curing, variable E-modulus, minimized leaching of uncured components [1] and suppressed non-specific binding of biomolecules [2]. This process is potentially suited for both rapid prototyping in the laboratory and medium-scale commercial production, bridging the “development gap”.
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| 7. |
- Chang, S, et al.
(författare)
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An electrowetting-based microfluidic platform for magnetic bioassays
- 2010
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Ingår i: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2010, 3-7 Oktober, Groningen, Neterlands. - 9781618390622 ; 2, s. 1331-1333
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Konferensbidrag (refereegranskat)abstract
- Here we present our recent work on a droplet-based microfluidic device for manipulating microliter-sized droplets. By replacing the formerly used common dielectric SiO2 with Si3N4 and applying a 33 nm thick Teflon top layer to create a hydrophobic surface, we successfully lowered the actuation voltage from 450 V to 50 Vdc/40 Vac. Sputtered HfO2 with high dielectric constant was also investigated as an insulator, which could reproducibly yield thin defect-free insulation layers and lower the actuation voltage to less than 40 V.
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| 8. |
- Frisk, T., et al.
(författare)
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Live-cell imaging of natural killer cell mediated tumor rejection in arrays of microwells
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; , s. 950-952
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Konferensbidrag (refereegranskat)abstract
- Due to the inherent heterogeneity of immune cell populations a comprehensive view of immune functions can only be achieved by collecting data from many individual cells. We here demonstrate a novel multi-well microchip platform for cell analysis, which we use to study the interaction between natural killer (NK) cells and their target cells.
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| 9. |
- Jönsson, Håkan, et al.
(författare)
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A homogeneous assay for biomolecule interaction analysis in droplets by flourescence polarization
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; , s. 1802-1804
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Konferensbidrag (refereegranskat)abstract
- We present a novel homogeneous assay for detecting biomolecule interactions in microdroplets by fluorescence polarization (FP) for the first time. The FP assay allows the detection of target biomolecules directly after incubation without removing the detection reagent by separation or washing, making the assay amenable to automation. Using this assay we evaluate protein-protein and drug-DNA interactions. We detect these interactions at concentrations as low as 100nM and 69 pM respectively. This is a proof-of-concept homogeneous labeling assay in droplets for detecting bio-macromolecules.
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| 10. |
- Jönsson, Håkan, et al.
(författare)
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Deterministic lateral displacement device for droplet separation by size - Towards rapid clonal selection based on droplet shrinking
- 2010
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Ingår i: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. - 9781618390622 ; , s. 1355-1357
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Konferensbidrag (refereegranskat)abstract
- We present a novel method for robust passive separation of microfluidic droplets by size using deterministic lateral displacement(DLD). We also show that droplets containing Saccharomyces Cervisiae shrink significantly during incubation while droplets containing only yeast media retain their size. We demonstrate the DLD device by sorting out shrunken yeast-cell containing droplets from a 40-fold excess of ∼33% larger yeast-cell-free droplets generated at the same time, suggesting that DLD might be used for clonal selection. The same device also separates 11 μm from 30μm droplets at a rate of 12000droplets/second, more than twofold faster than previously demonstrated passive hydrodynamic separation devices [1].
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