| 1. |
- Barnkob, Rune, et al.
(författare)
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Measuring density and compressibility of white blood cells and prostate cancer cells by microchannel acoustophoresis
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; 1, s. 127-129
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Konferensbidrag (refereegranskat)abstract
- We present a novel method for the determination of density and compressibility of individual particles and cells undergoing microchannel acoustophoresis in an arbitrary 2D acoustic field. Our method is a critical advancement within acoustophoretic separation of biological cells, as the ability to determine the density and compressibility of individual cells enables the prediction and alteration of the separation outcome for a given cell mixture. We apply the method on white blood cells (WBCs) and DU145 prostate cancer cells (DUCs) aiming to improve isolation of circulating tumor cells from blood, an emerging tool in the monitoring and characterizing of metastatic cancer.
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| 2. |
- Beech, Jason P., et al.
(författare)
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Cell morphology and deformability in deterministic lateral displacement devices
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; 2, s. 1355-1357
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Konferensbidrag (refereegranskat)abstract
- Deterministic Lateral Displacement (DLD) devices have been used to separate particles based on size [1] and shape [2]. Here we show how DLD devices can also be used to separate particles based on their ability to deform under shear forces. Varying experimental conditions allows us to vary the relative contributions of size, morphology and deformability. The ability to distinguish between cells based on deformability with high resolution and throughput, in cheap and simple devices, could find highly interesting and relevant applications, for example in the detection of circulating tumor cells or malaria-infected blood cells.
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| 3. |
- Carlborg, Carl Fredrik, et al.
(författare)
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Biosticker : patterned microfluidic stickers for rapid integration with microarrays
- 2011
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Ingår i: The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2011). - : Chemical and Biological Microsystems Society. - 9781618395955 ; , s. 311-313
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Konferensbidrag (refereegranskat)abstract
- We present a one-step, reversible, and biocompatible bonding method of a stiff patterned microfluidic "Biosticker", based on off-stoichiometry thiol-ene (OSTE) polymers [1], to state-of-the-art spotted microarray surfaces. The method aims at improving and simplifying the batch back-end processing of microarrays. We illustrate its ease of use in two applications: a high sensitivity flow-through protein assay; and a DNA-hybridization test. Read-out was performed in a standard highvolume array scanner, and showed excellent spot homogeneity and intensity. The Biosticker is aimed to be a plug-in for existing microarray platforms to enable faster protein assays and DNA hybridizations through mass transport optimization.
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| 4. |
- Carlborg, Carl Fredrik, 1981-, et al.
(författare)
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LOW TEMPERATURE “CLICK” WAFER BONDING OF OFF-STOICHIOMETRY THIOL-ENE (OSTE) POLYMERS TO SILICON
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2011). - 9781618395955 ; , s. 1143-1145
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Konferensbidrag (refereegranskat)abstract
- We present a low temperature (< 37°C) wafer-scale microfluidic batch packaging process using covalent, dry bonding of offstoichiometry thiol-ene polymers (OSTE), enabling rapid, bio-compatible integration of fluidics on wafer-scale in combination with excellent polymer properties.
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| 5. |
- Christakou, Athanasia. E., et al.
(författare)
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Aggregation and long-term positioning of cells by ultrasound in a multi-well microchip for high-resolution imaging of the natural killer cell immune synapse
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; , s. 329-331
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Konferensbidrag (refereegranskat)abstract
- In this study we investigate the ability of Natural Killer (NK) cells to form ultrasound-mediated intercellular contacts with target cells in a multi-well microdevice by high-resolution confocal-microscopy imaging of inhibitory immune synapses. Furthermore, we compare the NK-Target cell cluster migration with and without ultrasound actuation. Experiments indicate that clusters of cells are positioned and maintained centered in the wells for 17 hours when they are exposed continuously to ultrasound. Our system can be used for both screening high numbers of events in low resolution and also for high resolution imaging of long term cell-cell interactions.
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| 6. |
- Freitag, C., et al.
(författare)
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Meandering nanochannels for imaging of ultra-long DNA molecules
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; 3, s. 1758-1760
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Konferensbidrag (refereegranskat)abstract
- A new chip design for handling and studying chromosomal DNA is described. Folding the nanochannels enables us to image an entire yeast chromosome in a single field of view. Together with existing mapping techniques our new design opens up the possibility of directly analyzing entire chromosomes in a single field of view using fluorescence microscopy. This would enable, for example, identification of microorganisms on a single-cell basis and the complete physical mapping of the human genome, chromosome by chromosome.
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| 7. |
- Gantelius, Jesper, et al.
(författare)
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A ten-minute high density lateral flow protein microarray assay
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011. - 9781618395955 ; , s. 1176-1178
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Konferensbidrag (refereegranskat)abstract
- Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use lateral flow protein microarray device capable of sensitive (<50ng/ml) determination of antigen specific antibodies in less than ten minutes total assay time. Results were developed with gold nanobeads and could be recorded by a cell-phone camera or table top scanner. Excellent accuracy (AUC=99.4%) was achieved in comparison with an established glass microarray assay for 26 antigen-specific antibodies.
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| 8. |
- Holm, Stefan H., et al.
(författare)
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Multiple depths in a deterministic lateral displacement device for field-diagnosis of sleeping-sickness
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; 1, s. 527-529
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Konferensbidrag (refereegranskat)abstract
- We present a simple and inexpensive device capable of extracting and concentrating the parasite causing sleeping sickness from blood. The device is aimed at being used in rural resource depraved areas where the disease is endemic; therefore simplicity is of paramount importance. The device is based on deterministic lateral displacement with a single inlet and flow induced by a syringe. Through an intricate design with multiple depths, a cell free stream is created from the blood plasma into which the parasites are guided and subsequently collected in a dedicated reservoir for observation.
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| 9. |
- Jönsson, Håkan, et al.
(författare)
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Microfluidic droplet based enzyme variant screening : Towards improved enzymes for industrial applications
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; , s. 179-181
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Konferensbidrag (refereegranskat)abstract
- We present a microdroplet based assay for selection of efficient variants of bacterially produced amylase enzyme to improve these enzymes for industrial applications. Fluorescent analysis of α-amylase in droplets at relevant concentrations demonstrates the discrimination of wild type α-amylase at stressed and non-stressed conditions. Dielectrophoretic sorting enables enrichment of target droplets from 48% to 98.1%. Finally the viability and proliferation of Bacillus Subtilis in droplets is demonstrated. This demonstrates an enzyme analysis and screening assay in the microfluidic droplets format for selection of an industrially relevant enzyme and a basis for further enzyme selections where fluorogenic substrates are available.
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| 10. |
- Ohlin, Mathias, et al.
(författare)
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Controlling acoustic streaming in a multi-well microplate for improving live cell assays
- 2011
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Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; , s. 1612-1614
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Konferensbidrag (refereegranskat)abstract
- Acoustic streaming in a multi-well microplate is investigated using two different ultrasonic actuation frequency-schemes: Single-frequency and frequency-modulation. The streaming is tracked by the use of 1 μm fluorescent polymer beads and micro-particle image velocimetry. The suspension also contained human B cells for studying the acoustic trapping and aggregation performance simultaneously with the acoustic streaming. Our results show a significant difference in the acoustic streaming between the two ultrasonic actuation schemes. A rotational fluid flow speed decreased a factor of 30 when frequency-modulation was applied compared to single-frequency actuation without apparently interfering with the acoustic cell trapping function.
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