| 1. |
- Akbari, Elham, et al.
(författare)
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SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 1201-1202
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Konferensbidrag (refereegranskat)abstract
- Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.
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| 2. |
- Andersson, Klara, et al.
(författare)
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ANTISYMMETRIC ACTUATION INCREASES ACOUSTOPHORESIS PERFORMANCE
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 901-902
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Konferensbidrag (refereegranskat)abstract
- Antisymmetric, symmetric and standard actuation of acoustofluidic devices were compared in terms of focusability. By imaging the end part of an acoustophoresis separation channel while flowing fluorescent beads through it, we show that beads get more tightly focused by antisymmetric actuation than symmetric and standard actuation at a given electrical input power. This means that antisymmetric actuation is more suitable for single node acoustic focusing as lower input power can be used to reach the same level of performance, or higher throughput can be reached for the same power.
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| 3. |
- Havers, Megan, et al.
(författare)
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OPTIMISATION AND PERFORMANCE QUANTIFICATION ACOUSTIC TRAPPING USING SYNTHETIC NANOPARTICLES
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 903-904
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Konferensbidrag (refereegranskat)abstract
- Acoustic trapping is becoming increasingly popular as a tool to isolate and enrich biological nanoparticles [1] (particularly extracellular vesicles [2]), yet an inherent problem with acoustic trapping of nanoparticles is calibration of the trapping efficiency. This abstract presents in-house synthesized nanoparticles with different sound scattering properties, either rigid high density gold nanoparticles (AuNPs) as model system for inorganic nanoparticles (NPs) or synthetic lipid bilayer nano vesicles to mimic the acoustic properties of biological vesicles. The rapid NP synthesis facilitates characterisation of the trapping performance of a single node acoustic trapping system, e.g. by designing application specific NPs size ladders. This is shown by the trapping of AuNPs of two different sizes and synthetic lipid bilayer vesicles and demonstrating a 6-11% NP recovery. The work demonstrates the potential of freshly synthesized nanoparticles as a tool for optimisation of acoustic trapping protocols as well as a fast and simple NP trapping performance verification by visual inspection.
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| 4. |
- Liu, Zhenhua, 1992-, et al.
(författare)
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Time-controlled Microbead-based Reactions in Droplets using Acoustophoresis
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 893-894
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Konferensbidrag (refereegranskat)abstract
- Droplet microfluidics is a powerful method to characterize chemical reactions at high throughput. Often detection is performed via in-line optical readout, which puts high demands on the detection system and makes detection of low concentration substrates challenging. Here, we have developed a droplet acoustofluidic chip for time-controlled microbead-based reactions that is combined with off-line optical readout.
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| 5. |
- Pohlit, Hannah, et al.
(författare)
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ACOUSTOFLUIDIC METHOD TO ALIGN POLYSTYRENE BEADS AND CELLS IN HYDROGEL DROPLETS
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 887-888
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Konferensbidrag (refereegranskat)abstract
- We present a microfluidic system to control the position of particles (polystyrene beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves. Droplets comprising hydrogel precursor solution, photoinitiator and (bio-)particles were generated and acoustic forces focused the beads to the droplet center line. Droplets were cross-linked by exposure to UV-light. With the acoustics applied, 89 ± 19% of the particles were positioned in the center of the hydrogel droplet. As proof-of-principle for biological applications, astrocytes were focused in hydrogel droplets. The viability of the cells after 7 days was unaffected by the acoustic focusing (72 ± 22%).
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| 6. |
- Ström, Oskar E., et al.
(författare)
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KÁRMÁN VORTEX CITY OF DNA STRANDS
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 13-14
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Konferensbidrag (refereegranskat)abstract
- Kármán vortex streets are repeating patterns of swirling vortices, appearing when a fluid passes an obstacle at Re>50. We present an array of interacting vortices, a Kármán vortex city, that arises when a DNA solution flows through an array of micro posts at Re <0.5. Above thresholds in concentration and flow rate, the vortices formed behind posts interact, leading to concentration fluctuation waves. We explore the effect of lattice structure, polymer length and salt and relate the wave formation to the overlap concentration. Our work will allow us to optimize high-concentration DNA manipulation and sorting of DNA in microfluidic arrays.
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| 7. |
- Undvall, Eva, et al.
(författare)
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Inertia induced breakdown of acoustic sorting efficiency at high flow rates
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 895-896
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Konferensbidrag (refereegranskat)abstract
- We investigated the impact of inertial forces on acoustic sorting at high sample throughputs. Above a critical flow rate, we observed an unexpected inertia induced breakdown of the sorting efficiency that is not due to the wall induced lift force and was not previously described in the literature. We analyzed the effect experimentally and theoretically, providing an explanation to the phenomena, and suggest conditions to maximize the sample throughput, while keeping the sorting efficiency intact. The observed phenomena is generic to all separation techniques that rely on field based (electrical, magnetic, acoustic) forces acting on cells/particles.
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| 8. |
- Zou, Hanbang, et al.
(författare)
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Examining multiple-contact miscibility in reservoir drainage using a rock-on-a-chip
- 2021
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Ingår i: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419031 ; , s. 1667-1668
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Konferensbidrag (refereegranskat)abstract
- A series of experiments are carried out to exam the effect of multiple-contact miscibility on drainage using a rock-on-a-chip. The result shows the distinct behaviors depending on the composition and injection flow rate. The observations are compared with the classical parameter regime diagram for drainage displacement of R. Lenormand et al[1].
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