| 1. |
- Hebig, Regina, 1984, et al.
(författare)
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How do students experience and judge software comprehension techniques?
- 2020
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Ingår i: IEEE International Conference on Program Comprehension. - New York, NY, USA : ACM. ; , s. 425-435
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Konferensbidrag (refereegranskat)abstract
- Today, there is a wide range of techniques to support softwarecomprehension. However, we do not fully understand yet whattechniques really help novices, to comprehend a software system.In this paper, we present a master level project course on softwareevolution, which has a large focus on software comprehension. Wecollected data about student's experience with diverse comprehension techniques during focus group discussions over the course oftwo years. Our results indicate that systematic code reading canbe supported by additional techniques to guiding reading efforts.Most techniques are considered valuable for gaining an overviewand some techniques are judged to be helpful only in later stagesof software comprehension efforts.
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| 2. |
- Ågren, Magnus, 1984, et al.
(författare)
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Architecture evaluation in continuous development
- 2022
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Ingår i: Journal of Systems and Software. - : Elsevier BV. - 0164-1212. ; 184
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Tidskriftsartikel (refereegranskat)abstract
- Context: In automotive, stage-gate processes have previously been the norm, with architecture created mainly during an early phase and then used to guide subsequent development phases. Current iterative and Agile development methods, where the implementation evolves continuously, changes the role of architecture. Objective: We investigate how architecture evaluation can provide useful feedback during development of continuously evolving systems. Method: Starting from the Architecture Tradeoff Analysis Method (ATAM), we performed architecture evaluation, both in a national research project led by an automotive Original Equipment Manufacturer (OEM), and at the OEM, in the context of continuous development. This allows us to include the experience of several architects from different organizations over several years. Using data produced during the evaluations we perform a post-hoc analysis to derive initial findings. We then validate and refine these findings through a series of focus groups with architects and industry experts. Findings: We propose principles of continuous evaluation and evolution of architecture, and based on these discuss a roadmap for future research. Conclusion: In iterative development settings, the needs are different from what typical architecture evaluation methods provide. Our principles show the importance of dedicated feedback-loops for continuous evolution of systems and their architecture.
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| 3. |
- Almqvist, Sofia, 1980, et al.
(författare)
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Amelogenin is phagocytized and induces changes in integrin configuration, gene expression and proliferation of cultured human dermal fibroblasts
- 2010
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Ingår i: Journal of Materials Science. Materials in Medicine. - : Springer Science and Business Media LLC. - 1573-4838 .- 0957-4530. ; 21:3, s. 947-954
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Tidskriftsartikel (refereegranskat)abstract
- Fibroblasts are central in wound healing by expressing important mediators and producing and remodelling extracellular matrix (ECM) components. This study aimed at elucidating possible mechanisms of action of the ECM protein amelogenin on normal human dermal fibroblasts (NHDF). Amelogenin at 100 and 1000 μg/ml increased binding of NHDF via several integrins, including αvβ3, αvβ5 and α5β1. Further, both surface interaction and cellular uptake of amelogenin by NHDF was observed using scanning and transmission electron microscopy. Gene microarray studies showed >8-fold up or down-regulation of genes, of which most are involved in cellular growth, migration and differentiation. The effect of amelogenin was exemplified by increased proliferation over 7 days. In conclusion, the beneficial effects of amelogenin on wound healing are possibly conducted by stimulating fibroblast signalling, proliferation and migration via integrin interactions. It is hypothesized that amelogenin stimulates wound healing by providing connective tissue cells with a temporary extracellular matrix.
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| 4. |
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| 5. |
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| 6. |
- Almqvist, Sofia, 1980, et al.
(författare)
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Ex Vivo Study of the Angiogenic Effect of the Extracellular Matrix Protein Amelogenin
- 2008
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Ingår i: Abstract, The 9th New Jersey Symposium on Biomaterials Science and regenerative medicine, New Jersey, USA. ; 29-31 October
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Konferensbidrag (refereegranskat)abstract
- Introduction: Angiogenesis is crucial for wound healing but is often impaired in chronic wounds. The process is dependent on the interaction of endothelial cells and the extracellular matrix (ECM), which is mediated by cell membrane integrins. Amelogenin is an extracellular matrix protein that has been reported to promote formation of granulation tissue and repair of chronic venous leg ulcers and elevate the pro-angiogenic vascular endothelial growth factor in dermal fibroblasts.1-3 This study investigated the effect of amelogenin on angiogenesis in an ex vivo sprouting assay and related the findings to the cell surface integrin expression. Methods: Chick aortic arch assay: Transverse sections of the aortic arch of 13-day-old chick embryos were attached and sealed with Matrigel to the bottom of a 48-well plate. Amelogenin was added (0.01 mg/ml, 0.1 mg/ml and 1 mg/ml) in serum-free endothelial basal growth medium. Porcine serum albumin was used as control for unspecific protein effects. The plates were incubated at 37°C and sprouting was assessed at 24 h and 48 h by microscopy and scored from 0 to 6 (arbitrary units) by a blinded observer. Integrin assay: Human dermal microvascular endothelial cells (Promocell) were seeded in complete cell growth medium alone or supplemented with 0.1 mg/ml, 1 mg/ml amelogenin or 20 µg/ml fibronectin. After 24 h incubation in 37°C, cells were gently harvested with the non-enzymatic buffer (EDTA/PBS). Upregulated integrins/subunits were detected by an Integrin-Mediated Cell Adhesion Array (Chemicon), where cells expressing specific integrins (α1, α2, α3, α4, α5, αv, β1, β2, β3, β4, β6, αvβ3, αvβ5 and α5b1) are captured by surface immobilized antibodies. Results and Discussion: Amelogenin at 0.1 mg/ml significantly (p = 0.001) increased micro-vessel outgrowth by 76 % from the explants compared with control explants after 48 h of incubation. No significant sprouting was observed with the non-specific protein control porcine serum albumin or medium only. The preliminary data from the integrin assay show that amelogenin at 0.1 mg/ml also displays a broad up-regulation of several integrins/subunits. This result is comparable to the positive control, fibronectin, an ECM protein involved in all phases of tissue repair. Taken together, the present observations suggest that the angiogenic effects might be explained by the cell binding properties of amelogenin. Conclusions: Amelogenin stimulated micro-vessel outgrowth in the chick aortic arch assay possibly through up-regulation of several integrins and subunits important for cell interaction with the ECM. The pro-angiogenic property may contribute to the beneficial effects reported after treatment of chronic ulcers with the novel ECM therapy containing amelogenin. Acknowledgements: The studies were supported by the Swedish Research Council (grant K2006-73X-09495-16-3), Mölnlycke Health Care AB, the VINNOVA VinnVäxt Program Biomedical Development in Western Sweden, and the Danish Medical Research Council (22-02-0287). References: 1. Mirastschijski U. et al. (2004) Wound Repair Regen. 12:100-108. 2. Vowden P. et al. (2006) Wound Repair Regen. 14:240-248. 3. Ågren M. S. et al. (2007) Wound Repair Regen. 15:A139.
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| 7. |
- Almqvist, Sofia, 1980, et al.
(författare)
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In Vitro Effect of Amelogenin on Selected Cell Mediators from Human Monocytes
- 2008
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Ingår i: 8th World Biomaterials Congress, Amsterdam, The Netherlands.
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Konferensbidrag (refereegranskat)abstract
- Introduction: Inflammation is an integral part of the normal wound healing response. Besides clearing the wound of invading microbes and debris, inflammatory cells are believed to be crucial coordinators of the repair process, acting both as phagocytes and as a major source of growth factors and other signals [1]. In non-healing skin ulcers the repair process is stuck in the inflammation phase [2]. Excessive inflammation can reflect an imbalance in the transformation of phenotype between the classically activated, inflammatory macrophage and the alternatively activated macrophage involved in immunosuppression and tissue repair [3]. Amelogenin is a hydrophobic extracellular matrix protein that under physiological conditions will self assemble into nanospheres which in turn may form larger aggregates. Treatment with amelogenin has shown enhanced skin wound healing in an in vivo study in rabbits [4]. In addition, amelogenin has been proposed to have anti-inflammatory properties by attenuation of lipopolysaccharide (LPS)- and peptidoglucan-induced production of selected pro-inflammatory cytokines by human blood cells [5]. The present study was initiated to determine the effects of amelogenin on human monocyte secretion of factors which modulate both inflammation and tissue repair. Materials and Methods: Lyophilized amelogenin from Biora AB (Malmö, Sweden) was dissolved in 17 mM acetic acid. Human monocytes were obtained from six healthy blood donors by isolation using the separation gradient PercollTM in two steps according to Pertoft et al. [6]. The isolated monocytes were cultured for 24 h at 37ºC with 5% CO2 and 95% humidity. Thereafter the supernatants and non-adherent cells were removed. Fresh medium (RPMI, 5% foetal bovine serum, antibiotics) containing amelogenin, 0, 0.01, 0.1 and 1.0 mg/ml, and with or without addition of LPS, was added to the wells in triplicates. The plates were again incubated for 24 h. The supernatants were analyzed with commercial human ELISA assays for tumour necrosis factor- (TNF-), interleukin-10 (IL-10), macrophage inflammatory protein-1 (MIP-1), monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor (VEGF), and insulin like growth factor-1 (IGF-1). Results: Amelogenin treatment markedly altered the expression of factors by human monocytes. Amelogenin significantly reduced LPS-induced TNF- secretion, whereas the IL-10 expression was increased. Monocyte secretion of the two inflammatory chemokines MIP-1 and MCP-1 (Figure; mean ± SEM, n=6) was also affected by amelogenin treatment. Furthermore, amelogenin significantly increased monocyte secretion of VEGF (Figure; mean ± SEM, n=6) and IGF-1, although to a lesser extent, after 24 h culture. Conclusions: The amelogenin effects correlate to protein concentration, however not in a dose dependent manner, but instead the cell responses may reflect a concentration related difference in self assembly of the amelogenin protein. The observed changes in cytokine and chemokine expression are markedly affected by simultaneous LPS-induced inflammation activation, revealing possible anti-inflammatory properties of the amelogenin protein. In addition, the several-fold increase in VEGF-levels by monocytes provides a possible mechanism for the observed pro-angiogenic effect in vivo [4]. These in vitro results indicate that the extracellular matrix protein amelogenin by virtue of its interaction with human monocytes may modulate inflammation and tissue repair. Acknowledgements: The support from the Swedish Research Council (grant K2006-73X-09495-16-3), Mölnlycke Health Care Group AB and the VINNOVA VinnVäxt Program Biomedical Development in Western Sweden, is gratefully acknowledged. References: 1. Martin, P., et al. Trends Cell Biol., 15, 599, 2005. 2. Ågren, M.S., et al. Acta Derm Venereol Suppl (Stockh). 210, 3, 2000. 3. Duffield, J.S. Clin Sci (Lond), 104, 27, 2003 4. Mirastschijski, U., et al. Wound Repair Regen., 12, 100, 2004. 5. Myhre, A.E., et al. J Periodontal Res., 41, 208, 2006. 6. Pertoft, H., et al. J Immunol Methods., 33, 221, 1980.
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| 8. |
- Knauss, Eric, 1977, et al.
(författare)
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Continuous Integration Beyond the Team: A Tooling Perspective on Challenges in the Automotive Industry
- 2016
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Ingår i: Proceedings of the 10th ACM/IEEE International Symposium on Empirical Software Engineering and Measurement (ESEM '16). - New York, NY, USA : ACM. - 1949-3770 .- 1949-3789. - 9781450344272
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Konferensbidrag (refereegranskat)abstract
- The practice of Continuous Integration (CI) has a big impact on how software is developed today. Shortening integration and feedback cycles promises to increase software quality, feature throughput, and customer satisfaction. Thus, it is not a surprise that companies try to embrace CI in domains where it is rather difficult to implement. In this paper we present our findings from two rounds of interviews with a car manufacturer on the use of tools in system engineering and how these tools would support wider adoption of CI. Our findings suggest a complex tool landscape with immense requirements that are not easily fulfilled by existing tools; this holds also for tools that well support CI in other domains. From this notion, we fur- ther explore what makes the automotive domain challeng- ing when it comes to CI (namely complexity of system and value chain). We hope that our findings will help address such challenges.
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| 9. |
- Pelliccione, Patrizio, 1975, et al.
(författare)
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A proposal for an Automotive Architecture Framework for Volvo Cars
- 2016
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Ingår i: Workshop on Automotive Systems/Software Architectures (WASA), Venice, Italy, April 05-08, 2016. - 9781509025718 ; , s. 18-21
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Konferensbidrag (refereegranskat)abstract
- © 2016 IEEE. During the past twenty years vehicles have become more and more robot like, interpreting and exploiting input from various sensors to make decisions and finally commit actions that were previously made by humans. Such features will require continuous evolution and updates to ensure safety, security, and suitability for supporting drivers in an ever changing world. Modern vehicles can have over 100 Electronic Control Units (ECUs), which are small computers, together executing gigabytes of software. ECUs are connected to each other through severalnetworks within the car, and in some cases also to the outside world. This need for addressing ever increasing complexity as well as for offering flexibility, support of continuous evolution, and very late changes in user visible features introduces new challenges for developing and maintaining a suitable electronic architecture. In this paper we report the current investigation of the Volvo Cars to create an architecture framework tailored to the needs of future vehicles.
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| 10. |
- Pelliccione, Patrizio, 1975, et al.
(författare)
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Architecting cars as constituents of a system of systems
- 2016
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Ingår i: ACM International Conference Proceeding Series. - New York, NY, USA : Association for Computing Machinery (ACM). - 9781450363990 ; , s. 1-7
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Konferensbidrag (refereegranskat)abstract
- Future transportation systems will be a heterogeneous mix of items with varying connectivity and interoperability. A mix of new technologies and legacy systems will co-exist to realize a variety of scenarios involving not only connected cars but also road infrastructures, pedestrians, cyclists, etc. Future transportation systems can be seen as a System of Systems (SoS), where each constituent system - one of the units that compose an SoS - can act as a standalone system, but the cooperation among the constituent systems enables new emerging and promising scenarios. In this paper we investigate how to architect cars so that they can be constituents of future transportation systems. This work is realized in the context of two Swedish projects coordinated by Volvo Cars and involving some universities and research centers in Sweden and many suppliers of the OEM, including Autoliv, Arccore, Combitech, Cybercom, Knowit, Prevas, ÅF-Technology, Semcom, and Qamcom.
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