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- Espeland, Marianne, 1981-, et al.
(författare)
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Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
- 2010
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Ingår i: Frontiers in Zoology. - : Springer Science and Business Media LLC. - 1742-9994. ; 7:2, s. 1-8
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Tidskriftsartikel (refereegranskat)abstract
- Background: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, paradichlorobenzene and naphthalene on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time intervals. Results: The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not. Conclusion: Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should, however, be preferred due to physical damage of specimens and putative health risks by chemicals.
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- Åkerlund, Monika, et al.
(författare)
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Nitrogen treatment : An insect case study
- 2001
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Ingår i: Integrated Pest Management for Collections. Proceedings of 2001: A Pest Odyssey. - London.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Anoxic treatment was tested for pest control on three types of insect cases. Test animals were larvae of Trogoderma angustum (Solier) and Anthrenus verbasci (L). Cases were enclosed in low diffusion plastic and the oxygen level was reduced by use of the VELOXY nitrogen generator. Test was performed 1) with nitrogen treatment only, 2) with 2 sachets of Ageless 200 added during one and two weeks respectively and 3) with 4 and 8 sachets of Ageless 200 added during one week. Oxygen level was reduced to 0.2% by the VELOXY. Temperature was about 22º C and RH around 28% in the cases. In the treatment with VELOXY only, all test specimens died. With 2 Ageless 200 almost all insects survived one week. After two weeks a few larvae survived in the tightest case. With 4 Ageless 200, 3 Trogoderma survived in the tightest case. With 8 sachets all insects died.
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