| 1. |
- Börjesson, Stefan, 1979-, et al.
(författare)
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Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill
- 2022
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Ingår i: Frontiers in Microbiology. - : Frontiers Media S.A.. - 1664-302X. ; 13
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Tidskriftsartikel (refereegranskat)abstract
- Occurrence of multidrug resistant Enterobacteriaceae in livestock is of concern as they can spread to humans. A potential introduction route for these bacteria to livestock could be animal feed. We therefore wanted to identify if Escherichia spp., Enterobacter spp., Klebsiella spp., or Raoutella spp. with transferable resistance to extended spectrum cephalosporins, carbapenems or colistin could be detected in the environment at feed mills in Sweden. A second aim was to compare detected isolates to previous described isolates from humans and animals in Sweden to establish relatedness which could indicate a potential transmission between sectors and feed mills as a source for antibiotic resistant bacteria. However, no isolates with transferable resistance to extended-cephalosporins or colistin could be identified, but one isolate belonging to the Enterobacter cloacae complex was shown to be carbapenem-resistant and showing carbapenemase-activity. Based on sequencing by both short-read Illumina and long-read Oxford Nanopore MinIon technologies it was shown that this isolate was an E. asburiae carrying a bla IMI-2 gene on a 216 Kbp plasmid, designated pSB89A/IMI-2, and contained the plasmid replicons IncFII, IncFIB, and a third replicon showing highest similarity to the IncFII(Yp). In addition, the plasmid contained genes for various functions such as plasmid segregation and stability, plasmid transfer and arsenical transport, but no additional antibiotic resistance genes. This isolate and the pSB89A/IMI-2 was compared to three human clinical isolates positive for bla IMI-2 available from the Swedish antibiotic monitoring program Swedres. It was shown that one of the human isolates carried a plasmid similar with regards to gene content to the pSB89A/IMI-2 except for the plasmid transfer system, but that the order of genes was different. The pSB89A/IMI-2 did however share the same transfer system as the bla IMI-2 carrying plasmids from the other two human isolates. The pSB89A/IMI-2 was also compared to previously published plasmids carrying bla IMI-2, but no identical plasmids could be identified. However, most shared part of the plasmid transfer system and DNA replication genes, and the bla IMI-2 gene was located next the transcription regulator imiR. The IS3-family insertion element downstream of imiR in the pSB89A was also related to the IS elements in other bla IMI-carrying plasmids.
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| 2. |
- Casimir, Justin, et al.
(författare)
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Småskalighet som ett medel för att bana väg för framtidens livsmedel?
- 2018
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The food value chain system in Sweden is well established making it hard for small companies to develop new products and even harder to create new food supply systems Obstacles could lay at the beginning of the chain (food production or processing), at the end (marketing, consumer) or could even be related to the legislative regulation framing the food supply chain. Smaller actors often lack resources and networks to develop their sector. However, their degree of creativity, innovativeness, and engagement is high, and their energy is needed in the development of new sustainable food value chains.The aim of this project was to develop and apply a methodology for evaluating food value chains, focusing on profitable small-scale production systems in Sweden that show potential for fast development of new products that quickly reach the market. The work also included identifying Swedish raw food materials with growth potential and to identify how they could come into greater demand. Ten food value chains with high development potential in Sweden and for export were mapped and the main bottlenecks briefly described. Three food chains where selected based on a potential-difficulty-benefit matrix. The three selected food value chains were: (i) Hops, (ii) Swedish forest berries, and (iii) Land-based fish farming. These three food value chains where further studied looking at the whole value chain, from production to end consumer. Through literature review and contacts with relevant stakeholders (telephone interview, face-to-face interview, or workshop) the bottlenecks were clarified and potential solutions for increased demand where identified.Swedish hops production is carried out by passionate and engaged smaller actors, mostly on a hobby level, and the hops is used as an ingredient for beer. Germany and the USA produce about 75% of the worldwide production These hops varieties are not adapted to the Swedish climate and therefore result in a low volume and poor quality. However, domestic varieties have been grown in the past giving better yield under Swedish climate conditions. More work is needed to characterize the quality of Swedish hops. At the present time, knowledge about the characteristics of Swedish hops is low, explaining the lack of interest from brewers. In Sweden most hops are harvested by hand, making it nearly impossible to be profitable on the market. The mechanization of the harvesting step is necessary to move Swedish hops from a hobby to a commercial activity. No solutions are available on the Swedish market, RISE together with SLU is looking to develop a hops harvester fulfilling Swedish and EU regulations and adapted to small scale cultivation. At the end of the value chain, innovative products could increase the need for Swedish hops, for example by developing beers brewed with fresh hops. Moreover, hops have antiseptic characteristics and could potentially be used in other food products than beer.Only about four percent of the berries that are produced by the Swedish forests every year are picked. The largest volumes picked are for bilberry (Swedish: blåbär), lingonberry, and cloudberry and most of them are washed and frozen in Sweden. Processing of berries, however, has to a large extent moved out of the country while the products produced for the Swedish market are quite traditional, low-processed foods such as jams, juices and dessert soups. The majority of the Swedish berries mainly bilberries due to their nutritional content are exported and are further processed into value added powders or extracts in Asia and Europe. In Sweden this kind of value chain is under developed largely due to knowledge barriers and to the currently very traditional market. However, there is a great consumer interest in berries and they have a perceived healthiness also in Sweden. Consumers are also increasingly aware of the origin of berries used as ingredients in products such as jams, purees and juice, as well as in health food products. To fill this gap between consumer interest/demand and raw materials available new businesses can be developed. To facilitate such development there is a need for knowledge generation and transfer along the whole value chain (picking, processing, product development and consumer studies), which can be generated by starting up new innovation and research projects. It is also of importance to facilitate networking, for example in the ‘berry network’ (coordinated by RISE), as the creation of a new value chain will require different businesses to cooperate. Also, product development projects will need support for testing, pilot production, and possibly in finding investment funding for new equipment.Land-based fish farming is small in comparison to traditional fish farming in Sweden, but several actors see a great potential in this system which has a lower impact on the environment compared to conventional fish farms. For instance, the Swedish farmer federation (LRF) has invested in a land-based fish farm recently. As in other EU-countries, the number of active farms in Sweden is decreasing and some see the potential to recycle unused animal stables into fish farms. A major bottleneck for land-based fish farming is current legislation as it is based on conventional fish farming and therefore does not consider the environmental benefits of land-based systems. Knowledge should be spread to relevant authorities and policy makers to open a dialog and facilitate the development of a relevant regulatory framework. Regarding the production phase, access to sustainably produced feed and technical competence are lacking. Moreover, as the technology is costly learning through trial and error would not be recommended. A testbed dedicated to land-based fish farming could support companies who wish to try modifications to their system. Furthermore, smaller producers have difficulties in finding processing solutions for their products; e.g. slaughterhouses and conditioning. Two potential solutions would be to develop a land-based fish farm cooperative and/or mobile systems that could take care of smaller productions. Finally, the competition on the market is tough as land-based fishes are competing with large-scale conventional fish farms from Norway and Asia. To overcome this bottleneck, the sector could develop its own certification as well as increasing the consumers awareness and knowledge.Some conclusions could be applied to all the studied food chains. For instance, each value chain can be seen as a puzzle with many pieces. In order to develop new food value chains many separate pieces need to fall into place. Therefore, it is necessary to increase collaboration between stakeholders but also to have a stakeholder driven coordination of this collaboration. The stakeholders within the developing value chains often do not have all the resources to carry out this task, especially if they are small businesses. The development of cooperatives also seems to be a solution to overcome bottlenecks in the studied food chains. Likewise, logistics in the developing value chains have a great margin for improvement. Furthermore, this project focused on value chains where food commodities are the end product but investigating the potential for non-food uses would also be of interest.The method used in this project can be replicated to other value chains with potential of development. It would help the users to get a holistic view of the current bottlenecks and facilitate contact between stakeholders. The list of bottlenecks can be followed up and used as an indicator to evaluate if the value chain in moving forward.
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| 4. |
- Klingberg, Gunilla, et al.
(författare)
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How old are you? : a systematic review investigating the relationship between age and mandibular third molar maturity
- 2023
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 18:5, s. 1-14
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Forskningsöversikt (refereegranskat)abstract
- Introduction and objective: Radiographic evaluation of the maturity of mandibular third molars is a common method used for age estimation of adolescents and young adults. The aim of this systematic review was to examine the scientific base for the relationship between a fully matured mandibular third molar based on Demirjian's method and chronological age, in order to assess whether an individual is above or below the age of 18 years.Methods: The literature search was conducted in six databases until February 2022 for studies reporting data evaluating the tooth maturity using Demirjian´s method (specifically stage H) within populations ranging from 8 to 30 years (chronological age). Two reviewers screened the titles and abstracts identified through the search strategy independently. All studies of potential relevance according to the inclusion criteria were obtained in full text, after which they were assessed for inclusion by two independent reviewers. Any disagreement was resolved by a discussion. Two reviewers independently evaluated the risk of bias using the assessment tool QUADAS-2 and extracted the data from the studies with low or moderate risk of bias. Logistic regression was used to estimate the relationship between chronological age and proportion of subjects with a fully matured mandibular third molar (Demirjian´s tooth stage H).Results: A total of 15 studies with low or moderate risk of bias were included in the review. The studies were conducted in 13 countries and the chronological age of the investigated participants ranged from 3 to 27 years and the number of participants ranged between 208 and 5,769. Ten of the studies presented the results as mean age per Demirjian´s tooth stage H, but only five studies showed the distribution of developmental stages according to validated age. The proportion of subjects with a mandibular tooth in Demirjian´s tooth stage H at 18 years ranged from 0% to 22% among males and 0 to 16% in females. Since the studies were too heterogenous to perform a meta-analysis or a meaningful narrative review, we decided to refrain from a GRADE assessment.Conclusion: The identified literature does not provide scientific evidence for the relationship between Demirjian´s stage H of a mandibular third molar and chronologic age in order to assess if an individual is under or above the age of 18 years.
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