| 1. |
- Abrahamsson, Jonas, 1954, et al.
(författare)
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Associations between neutrophil recovery time, infections and relapse in pediatric acute myeloid leukemia
- 2018
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Ingår i: Pediatric Blood & Cancer. - : John Wiley & Sons. - 1545-5009 .- 1545-5017. ; 65:9
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundChildren with acute myeloid leukemia (AML) treated similarly show different toxicity and leukemic responses. We investigated associations between neutrophil recovery time after the first induction course, infection and relapse in children treated according to NOPHO-AML 2004 and DB AML-01. ProcedureNewly diagnosed patients with AML with bone marrow blast<5% between day 15 after the start of the treatment and the start of second induction course, and in complete remission after the second induction course were included (n=279). Neutrophil recovery time was defined as the time from the start of the course to the last day with absolute neutrophil count<0.5x10(9)/l. Linear and Cox regressions were used to investigate associations. ResultsNeutrophil recovery time after the first induction course was positively associated with neutrophil recovery time after the remaining courses, and longer neutrophil recovery time (25 days) was associated with increased risk of grade 3-4 infections (hazard ratio 1.4, 95% confidence interval [CI], 1.1-1.8). Longer neutrophil recovery time after the first induction (>30 days) was associated with the increased risk of relapse (5-year cumulative incidence: 48% vs. 42%, hazard ratio 1.7, 95% CI, 1.1-2.6) for cases not treated with hematopoietic stem cell transplantation in first complete remission. ConclusionLonger neutrophil recovery time after the first induction course was associated with grade 3-4 infections and relapse. If confirmed, this knowledge could be incorporated into risk stratification strategies in pediatric AML.
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| 2. |
- Abrahamsson, Jonas, 1954, et al.
(författare)
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Cholesteryl sulphate and phosphate in the solid state and in aqueous systems.
- 1977
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Ingår i: Chemistry and physics of lipids. - 0009-3084. ; 19:3, s. 213-22
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Tidskriftsartikel (refereegranskat)abstract
- Cholesteryl sodium sulphate (CS) crystallizes as the dihydrate, the crystal structure of which is known. On heating the dihydrate, solid state phase transitions are observed at 65 degrees C and 95 degrees C and melting occurs at 165 degrees C. The structure of the high temperature phase has not been determined. Cholesteryl dihydrogen phosphate (CP) is not isostructural with any phases of CS. It undergoes a phase transition at 50 degrees C and melts at 190 degrees C. In systems with water CS is unstable whereas it was possible to determine the phase diagram of CP. In most of the composition range a crystalline hydrate is in equilibrium with a gel-phase. The latter has remarkable properties in that lamellar order exists with the 46 A lipid bilayer interleaved with water layers up to 1000 A. The monofilm behaviour of CS and CP at different pH levels is also reported.
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| 3. |
- Eliasson, Charlotte, 1973, et al.
(författare)
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Multivariate evaluation of doxorubicin surface-enhanced Raman spectra.
- 2001
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Ingår i: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. - 1386-1425. ; 57:9, s. 1907-15
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Tidskriftsartikel (refereegranskat)abstract
- Multivariate evaluation of surface-enhanced Raman spectra of doxorubicin in plasma was performed. In a principal component analysis (PCA) all spectral features were modelled into three principal components. The major variation of the data was shown to be the variation of doxorubicin Raman signal together with the doxorubicin fluorescence, whereas the variation due to plasma was of minor importance. It was also shown that the surface-enhanced Raman scattering (SERS) measurements were independent on such factors as measurement occasion and silver colloids. The presented results show that with some improvements, quantification of doxorubicin directly in plasma could be possible.
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| 4. |
- Eliasson, Charlotte, 1973, et al.
(författare)
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Multivariate methodology for surface enhanced Raman chemical imaging of lymphocytes
- 2006
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Ingår i: Chemometrics and Intelligent Laboratory Systems. - : Elsevier BV. - 0169-7439 .- 1873-3239. ; 81:1, s. 13-20
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Tidskriftsartikel (refereegranskat)abstract
- Surface enhanced Raman spectroscopy (SERS) was used to study the uptake of rhodamine 6G in human lymphocytes. In total four Raman images of lymphocytes were used. The aim was to find a multivariate methodology capable of separating spectra with chemical information from those that mainly contained the surface enhanced background, in order to create chemical images. The standard PCA procedure was compared with PCA of standard normal variate (SNV) corrected spectra, spectra baseline corrected in the wavelet domain, and variable trimming before PCA, to isolate unique spectra. It was not straightforward to perform a standard PCA for overview, since the small background variation in many variables dominated over the Raman band variation that only occur in few variables. It was shown that wavelet filtering could remove background variations and that variable trimming followed by PCA modelling left the unique Raman spectra as outliers, which facilitated interpretation of the Raman score images.
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| 5. |
- Eliasson, Charlotte, 1973, et al.
(författare)
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Surface-enhanced Raman scattering imaging of single living lymphocytes with multivariate evaluation
- 2005
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Ingår i: Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. - : Elsevier BV. - 1386-1425. ; 61:4, s. 755-760
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Tidskriftsartikel (refereegranskat)abstract
- This paper is aimed to show the possibility to determine individual organic compounds introduced into single living cells with surface-enhanced Raman spectroscopy (SERS). Surface enhancement was achieved with gold colloids that were allowed to diffuse into lymphocytes. An introduced analyte, rhodamine 6G, could be imaged together with for example nucleotides and amino acids of the cell. Multivariate evaluation of surface-enhanced Raman images proved to be a powerful tool for the separation of spectral information of various intracellular components. The principal component analysis (PCA) enabled identification of spectra containing different chemical information and separation of the spectral contribution of rhodamine 6G from the complex cellular matrix.
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| 6. |
- Lorén, Anders, 1974, et al.
(författare)
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Feasibility of quantitative determination of doxorubicin with surface-enhanced Raman spectroscopy
- 2001
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Ingår i: Journal of Raman Spectroscopy. - : Wiley. - 0377-0486 .- 1097-4555. ; 32:11, s. 971-974
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Tidskriftsartikel (refereegranskat)abstract
- Surface-enhanced Raman spectroscopy (SERS) was performed using excitation at 488 nm in a blood plasma-doxorubicin-silver colloid system. With a blood plasma content of 1%, a partial least-squares calibration of the doxorubicin was made in the 10-750 nM range. Predictions for a test set generated a root mean square error of prediction of 70 nM. The use of SERS and chemometrics in complex systems made it possible to use the highly informative Raman signals even at low concentrations without the need for sample pretreatment such as extraction. Copyright © 2001 John Wiley & Sons, Ltd.
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| 7. |
- Lorén, Anders, 1974, et al.
(författare)
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Internal standard in surface-enhanced Raman spectroscopy
- 2004
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 76:24, s. 7391-7395
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Tidskriftsartikel (refereegranskat)abstract
- A method is presented for the use of SAM layers as internal standards for calibration in surface-enhanced Raman spectroscopy. Three cyano-containing compounds were attached to gold colloids via a metal-sulfur bond and evaluated for spectral stability and normalization capacity. The results show that the analyte, rhodamine 6G, and the internal standard signal enhancement covaried, and it was possible to quantify the analyte with PLS. The fact that the enhancing substrate was chaotic assemblies with large variation in signal enhancement shows the versatility of this method.
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| 8. |
- Lorén, Anders, 1974, et al.
(författare)
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Self-assembled monolayer coating for normalization of surface enhanced Raman spectra
- 2004
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Ingår i: Nano Letters. - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 4:2, s. 309-312
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate that the use of a self-assembled monolayer, consisting of a thiol derivative of Dabcyl, can be used to normalize surface enhanced Raman signals (SERS) with respect to varying enhancement. Chaotic assemblies of gold nanoparticles exhibit large spatial variation in enhancement. Our work shows that in such a system the signals from the reporting molecules in the SAM co-vary with the signal from the analyte solution. With this knowledge, a normalization procedure was used to increase the precision of the analyte signal by 1 order of magnitude, to 8-13%, fully acceptable for quantitative work.
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| 9. |
- Abildgaard, Lotte, et al.
(författare)
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Optimal treatment intensity in children with Down syndrome and myeloid leukaemia: data from 56 children treated on NOPHO-AML protocols and a review of the literature.
- 2006
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Ingår i: Annals of hematology. - : Springer Science and Business Media LLC. - 0939-5555 .- 1432-0584. ; 85:5, s. 275-80
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Tidskriftsartikel (refereegranskat)abstract
- Children with Down syndrome (DS) and myeloid leukaemia have a significantly higher survival rate than other children, but they also experience considerable treatment-related toxicity. We analysed data on 56 children with DS who were treated on the Nordic Society for Paediatric Haematology and Oncology-acute myeloid leukaemia (NOPHO-AML)88 and NOPHO-AML93 protocols and reviewed the literature. In the dose-intensive NOPHO-AML88 protocol, 8 out of 15 patients (53%) experienced an event. In the less dose-intensive NOPHO-AML93 protocol, 7 out of 41 patients (17%) had an event. Therapy was reduced in 29 patients (52%) with in average 75% and 67% of the scheduled dose of anthracycline and cytarabine, respectively. Treatment-related death occurred in seven who all received full treatment. Relapse and resistant disease occurred at a similar rate in those receiving full and reduced treatment. Review of major series of myeloid leukaemia of DS showed no clear relationship between dose and survival; however, it appears that both a reduction in treatment dose and a less intensively timed treatment regimen improved the outcome. Further studies are needed to define the optimal regimen for treating myeloid leukaemia of DS.
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| 10. |
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