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- de Peppo, Giuseppe Maria, 1981, et al.
(författare)
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Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
- 2014
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Ingår i: International journal of nanomedicine. - : Informa UK Limited. - 1176-9114 .- 1178-2013. ; 9:1, s. 2499-2515
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Tidskriftsartikel (refereegranskat)abstract
- Background: Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role in the osseointegration of implantable devices. Methods: In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Results: We found that the proliferation and osteogenic differentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion. Conclusion: Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration.
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| 5. |
- Agheli, Hossein, 1965
(författare)
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Nanostructured Artificial Biointerfaces
- 2006
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- AbstractThe interactions occurring between biological systems and the surface of biomaterials hasbeen an area of strong research focus for a number of decades. There has been a realisation ofthe importance of macromolecular interactions and a consequent need to understandbiointerfacial events at the nanometer length-scale. A set of tools for working at thenanometer scale are critical for the study, design and evaluation of new biomaterials forapplication to improve medical devices and therapies and there has been a significant researchdrive to develop engineering approaches able to perform in the nanometer regime. One suchengineering approach to nanopattern model biomaterial interfaces over large areas is colloidallithography. In this thesis work colloidal lithography is extended from metals and ceramicsfor use with organic materials including bulk homopolymers, thin films of bothhomopolymers and block copolymers and monolayers of proteins. Large area arrays oftopographic nanostructures with a range of aspect ratios and shapes are demonstrated bysystematic control of processing parameters. Templating of block copolymers via topographicor chemical nanopatterns was shown and a templated PLL-g-PEG layer was subsequentlyused to create large area nanopatterns of the protein Laminin. A novel approach to quantifyingprotein binding from AFM height histograms was developed and utilised to characterise thefunctional properties of the surface bound Laminin. Artificial nanostructured biointerfacesproduced by these approaches giving nanoscale topographic or chemical cues were used tostudy the influence of nanoscale surface structures on the behaviour of adherent cells inculture. Significant changes in cellular morphology and cytoskeletal arrangement wereobserved which correlated to reduced focal adhesion formation. Cells adherent tonanopatterns of protein adhesive domains and high aspect ratio (
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| 6. |
- Agheli, Hossein, 1965, et al.
(författare)
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Nanostructured biointerfaces
- 2006
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Ingår i: Materials Science and Engineering C. ; 26, s. 911-917
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Tidskriftsartikel (refereegranskat)
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| 10. |
- Ballo, Ahmed, 1978, et al.
(författare)
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Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants
- 2011
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Ingår i: International Journal of Nanomedicine. - 1178-2013 .- 1176-9114. ; 6, s. 3415-28
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Tidskriftsartikel (refereegranskat)abstract
- An implantable model system was developed to investigate the effects of nanoscale surface properties on the osseointegration of titanium implants in rat tibia. Topographical nanostructures with a well-defined shape (semispherical protrusions) and variable size (60 nm, 120 nm and 220 nm) were produced by colloidal lithography on the machined implants. Furthermore, the implants were sputter-coated with titanium to ensure a uniform surface chemical composition. The histological evaluation of bone around the implants at 7 days and 28 days after implantation was performed on the ground sections using optical and scanning electron microscopy. Differences between groups were found mainly in the new bone formation process in the endosteal and marrow bone compartments after 28 days of implantation. Implant surfaces with 60 nm features demonstrated significantly higher bone-implant contact (BIC, 76%) compared with the 120 nm (45%) and control (57%) surfaces. This effect was correlated to the higher density and curvature of the 60 nm protrusions. Within the developed model system, nanoscale protrusions could be applied and systematically varied in size in the presence of microscale background roughness on complex screw-shaped implants. Moreover, the model can be adapted for the systematic variation of surface nanofeature density and chemistry, which opens up new possibilities for in vivo studies of various nanoscale surface-bone interactions.
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