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| 2. |
- Ahgren, P, et al.
(författare)
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High-resolution frequency analysis with small data record
- 2000
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Ingår i: ELECTRONICS LETTERS. - : IEE-INST ELEC ENG. - 0013-5194. ; 36:20, s. 1745-1747
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Tidskriftsartikel (refereegranskat)abstract
- In a paper published recently Samant and Shearman discuss several techniques for performing spectral analysis using short data records to overcome the problem of caller ID identification. They fail, however, to mention the nonlinear least squares (NLS) me
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| 3. |
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| 4. |
- Othberg, A, et al.
(författare)
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Specific effects of platelet derived growth factor (PDGF) on fetal rat and human dopaminergic neurons in vitro
- 1995
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Ingår i: Experimental Brain Research. - 0014-4819. ; 105:1, s. 22-111
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Tidskriftsartikel (refereegranskat)abstract
- The neurotrophic effects of the BB isoform of platelet-derived growth factor (PDGF) on rat and human fetal mesencephalic dopaminergic neurons have been characterized in vitro. A dose-response analysis demonstrated maximal responses at 30 ng/ml of PDGF-BB. This concentration resulted in a marked increase in the survival and neurite outgrowth from rat and human tyrosine hydroxylase-(TH) positive, presumed dopaminergic neurons after 7 days in vitro. The effects of PDGF-BB on survival of TH-positive neurons were comparable to those of brain-derived neurotrophic factor (BDNF), whereas neurite outgrowth was more pronounced after addition of BDNF. The combination of BDNF and PDGF-BB yielded no additive effects. Double immunohistochemical staining of rat cultures demonstrated PDGF beta-receptors on about 90% of the TH-positive neurons. PDGF-BB treatment of rat mesencephalic cultures induced an upregulation of c-fos and TH mRNA with maximal levels after 0.5-2 h as assessed by quantitative PCR analysis. An increased number of Fos protein-positive cells was detected immunohistochemically after 4 h of PDGF-BB treatment. The present results provide further evidence for specific and direct effects of PDGF-BB on gene expression, survival and neurite outgrowth of mesencephalic dopaminergic neurons of rat and human origin.
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| 5. |
- Sjöborg, M, et al.
(författare)
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Expression of platelet-derived growth factor after intrastriatal ibotenic acid injury.
- 1998
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Ingår i: Experimental brain research. - 0014-4819. ; 119:2, s. 245-50
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Tidskriftsartikel (refereegranskat)abstract
- The expression of platelet-derived growth factor (PDGF) was studied in a rat model of Huntington's disease, produced by unilateral intrastriatal ibotenic acid injections. The most pronounced effect registered was that the number of PDGF-immunoreactive cells increased in the lesioned area up to 10 weeks after the surgery. Double immunofluorescence staining indicated that the PDGF-positive cells were astrocytes. The increased PDGF immunoreactivity was associated with only minor changes in total PDGF mRNA and PDGF protein levels in the lesioned area. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated a slight increase in PDGF mRNA after ibotenic acid lesion, but this was not reflected in an increase in PDGF A- and B-chain protein concentration as measured with ELISA. After sham operation an increase in PDGF protein concentration was seen, while the number of PDGF-immunoreactive cells was unchanged. The accumulation of PDGF in the astrocytes might reflect the role of PDGF in a repair process in neurodegenerative processes.
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