| 1. |
- Alm, Malin, et al.
(författare)
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Corticosterone metabolites in laying hen droppings-Effects of fiber enrichment, genotype, and daily variations
- 2014
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Ingår i: Poultry Science. - : Elsevier BV. - 0032-5791 .- 1525-3171. ; 93, s. 2615-2621
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Tidskriftsartikel (refereegranskat)abstract
- There is growing interest and concern for animal welfare in commercial poultry production. To evaluate stress and welfare in an objective and noninvasive way, fecal corticosterone metabolites (FCM) in droppings can be analyzed. However, the influence of diet, genotype, and daily variations in FCM and production of droppings in laying hens has been poorly investigated. This study examined the effect of insoluble fiber by adding 3% ground straw pellets to the feed to Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) hens housed in furnished cages between 20 and 40 wk of age. In total, 960 hens were included in the study. Droppings were collected 4 times per day for 3 consecutive days and analyzed by corticosterone immunoassay. Biological validation confirmed the ability of the assay to detect changes in FCM levels. Inclusion of straw pellets in the feed increased FCM concentration in both hen genotypes and increased excretion rate of FCM in LB hens. The LB hens also produced greater amounts of droppings than LSL hens. Both FCM levels and production of droppings varied during the day, although no distinct diurnal rhythm was found. These findings demonstrate that when using FCM to evaluate stress and welfare in laying hens, many factors (e.g., diet, genotype used, and so on) need to be taken into account to allow accurate interpretation of the results. In addition, under certain conditions, excretion rate of FCM might be more appropriate to use compared with FCM concentration.
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| 2. |
- Alm, Malin, et al.
(författare)
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Mussel shells as an environment enrichment and calcium source for floor-housed laying hens
- 2017
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Ingår i: Journal of Applied Poultry Research. - : Elsevier BV. - 1056-6171 .- 1537-0437. ; 26, s. 159-167
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Tidskriftsartikel (refereegranskat)abstract
- Feather pecking is still a behavioral problem in laying hen flocks, resulting in impaired welfare and economic losses. Environment enrichment, e.g., providing access to litter, to increase foraging behavior in laying hens has been shown to decrease feather pecking. This study investigated the effect of spreading crushed mussel shells (particle size 10 to 20 mm) in the litter area as an environment enrichment and source of calcium for layers receiving a commercial feed and for layers given a feed with 40% less calcium. These treatments were compared with a control involving hens given the commercial feed, but no mussel shells in the litter. The study included 900 Dekalb White layers housed in a single-tier floor system during a production cycle (20 to 72 wk of age). The mussel shells were consumed to a lower extent than predicted, resulting in calcium deficiency in hens fed the low-calcium diet. This in turn was evident as increased degree of keel bone deviations and lower eggshell breaking strength. Daily addition of crushed mussel shells to the litter for laying hens on a balanced or calcium-deficient diets did not generate any positive effects on bird feather cover, eggshell quality, production performance, or fearfulness. Hence, with the particle size of mussel shells used in this study, there was no indication of improved welfare due to environment enrichment.
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| 3. |
- Alm, Malin, et al.
(författare)
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Short term deprivation of the litter area after transfer to the layer facility - effects on welfare and production in pullets
- 2013
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Ingår i: World's Poultry Science Journal. - 0043-9339. ; , s. 38-38
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- In order to enable hens to find feed and water and to reduce the number of floor-laid eggs in floor systems, producers sometimes exclude pullets from the litter area for some time when they are moved into the production system. This could have negative consequences since litter is an important resource for layers and the procedure is not allowed in Sweden. The aim of the present study was to investigate how bird welfare and production are affected by deprivation of access to litter and space at introduction of pullets to the laying facility. The study comprised 600 floor reared Bovans Robust layers obtained at 16 weeks of age (wks) and housed in 6 groups of 100 in a single-tier floor laying system. Three groups had full access to the litter area during the whole study (OPEN) and three groups were excluded from the litter during the first two weeks (CLOSED) and then received full access throughout the rest of the study. The experiment will run a full production cycle (75 wks) and figures presented below show the results obtained until 51 wks as it is still in progress. Feather cover (6-24 points), pecking wounds, cleanliness, foot condition and keel bone deviations (all 1-4 points) were assessed by integument scoring at 40 wks. Fearfulness was assessed by testing novel object (NO) and tonic immobility (TI). Based on group means, the data were analyzed by analysis of variance (ANOVA) using the MIXED procedure of the statistical system (SAS 9.2). The feather cover score was higher in treatment CLOSED (17.9 points) compared to OPEN (12.3 points) (P=0.019). No difference was found between treatments in any of the other integument score parameters. Fearfulness was higher in groups in the OPEN treatment as compared to CLOSED, indicated by less approaches in the NO test (21.5 vs 48.0) (P<0.001) and longer duration of TI (11.3 min vs 6.4 min) (P=0.018). Rate of lay, number of mislaid eggs, proportion of cracked or dirty eggs and mortality were recorded regularly but no significant differences between treatments were shown. Thus, excluding hens from the litter area during the first two weeks in this type of production system did not decrease the number of floor laid eggs, but had a positive impact on feather cover and resulted in less fearful birds. Additional welfare parameters will be analyzed later on which may explain these results further.
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| 4. |
- Alm, Malin, et al.
(författare)
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Welfare and performance in layers following temporary exclusion from the litter area on introduction to the layer facility
- 2015
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Ingår i: Poultry Science. - : Elsevier BV. - 0032-5791 .- 1525-3171. ; 94, s. 565-573
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Tidskriftsartikel (refereegranskat)abstract
- When introduced to the laying facility, pullets are sometimes temporarily excluded from the litter area in order to help them locate food and water, and to prevent floor-laid eggs. This procedure is not permitted in Sweden, because it involves denying access to both litter and space, which may have a negative effect on bird welfare. The present study investigated how the welfare and performance of layers were affected by this temporary exclusion on introduction of hens to the laying facility. The study included 600 floor-reared Dekalb White layers obtained at 16wk age and housed in 6 groups of 100 in a conventional single-tier floor-laying system. Birds were either given full access to the litter area during the whole study or were excluded from the litter area during the first 2 wk after transfer to the laying facility. From 18 to 72 wk age, birds in both treatments had full access to the litter area. Excluding birds from the litter area for 2wk resulted in better feather cover and reduced fearfulness, according to novel object and tonic immobility tests. Furthermore, birds initially excluded from the litter area produced eggs with a lower proportion of shell irregularities than birds with full access to the litter area throughout. No difference was found in corticosterone metabolites in droppings rate of lay, mortality, or proportion of floor-laid eggs. In conclusion, none of the parameters studied indicated that the welfare of laying hens was compromised by temporary exclusion from the litter area on introduction to the laying facility. In fact, some of the data suggested that bird welfare had improved.
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| 5. |
- Alm, Malin, et al.
(författare)
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Welfare indicators in laying hens in relation to nest exclusion
- 2016
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Ingår i: Poultry Science. - : Elsevier BV. - 0032-5791 .- 1525-3171. ; 95, s. 1238-1247
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Tidskriftsartikel (refereegranskat)abstract
- Consumer concerns about the welfare of laying hens are increasing, leading to increased interest in identifying reliable ways to assess welfare. The present study evaluated invasive and non-invasive welfare indicators in relation to a stressful challenge. The study included 126 Lohmann Selected Leghorn hens housed in furnished cages. Welfare indicators were measured between 61 and 70 wk of age in birds excluded from their nests for 5 consecutive d and control birds that had continuous access to nests. Baseline recordings were carried out in both groups prior to and post exclusion period. The assessed indicators were: corticosterone metabolites in droppings (FCM), corticosterone concentration in yolk, corticosterone concentration in plasma, irregularities of eggshells, heterophil to lymphocyte (H:L) ratio, tonic immobility duration, and feather cover. Behavioral observations showed that the birds had a clear preference for using the secluded nest sites, confirming that they were likely to perceive nest exclusion as an undesirable experience. Further, elevated levels of FCM in droppings, yolk corticosterone concentrations, H:L ratios and irregular eggshells were detected in both nest deprived and control birds during the exclusion. This suggests that these indicators were able to detect an increased stress response arising from nest deprivation, and it is hypothesized that the stress spread to birds in adjacent cages with access to nests. There was a positive and consistent correlation between FCM in droppings and eggshell irregularities, also supporting the use of eggshell irregularities as a potential non-invasive welfare indicator. However, the pattern of the stress response varied between indicators and correlations were generally few and inconsistent, highlighting the complexity of the relationship among welfare indicators.
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| 6. |
- Alves Dias, Joana, et al.
(författare)
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Plasma variation and reproducibility of oxidized LDL-cholesterol and low-grade inflammation biomarkers among participants of the Malmö Diet and Cancer cohort
- 2016
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Ingår i: Biomarkers. - 1354-750X. ; 21:6, s. 562-571
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Tidskriftsartikel (refereegranskat)abstract
- Context: Large epidemiological studies often collect non-fasting samples, although the reliability of biomarkers may be uncertain. Objective: To explore the reliability and reproducibility of a single measurement of selected biomarkers in a sub-sample of the Malmö Diet and Cancer cohort. Methods: We estimated single- and average-measures intraclass correlation coefficients (ICC) for oxidized (ox)-LDL, interleukin (IL)-1β, IL-6, IL-8 and TNF-α. Results: Single-measures ICC in non-fasting samples of ox-LDL, IL-1β, IL-6, IL-8 and TNF-α were the following: 0.85, 0.71, 0.61, 0.78 and 0.66 for men, and 0.67, 0.81, 0.87, 0.69 and 0.81 for women. Biomarkers at non-fasting and fasting samples were highly correlated (all r > 0.80). Conclusions: The observed ICC suggest that most of the examined biomarkers (non-fasting blood) would allow meaningful analysis in epidemiological studies.
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| 10. |
- Björkbacka, Harry, et al.
(författare)
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Low Levels of Apolipoprotein B-100 Autoantibodies Are Associated With Increased Risk of Coronary Events.
- 2016
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Ingår i: Arteriosclerosis, Thrombosis and Vascular Biology. - 1524-4636. ; 36, s. 765-771
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: Previous smaller studies have indicated inverse associations between autoantibodies to oxidized low-density lipoprotein epitopes, and cardiovascular disease. The present study investigated associations between autoantibodies against the apolipoprotein B-100 peptides p45 and p210, respectively, and risk of incident cardiovascular disease in a large population-based cohort. APPROACH AND RESULTS: Apolipoprotein B-100 autoantibodies were analyzed by ELISA in a prospective study, including 5393 individuals (aged 46-68 years) belonging to the cardiovascular arm of the Malmö Diet and Cancer study with a follow-up time of >15 years. Subjects that suffered an acute coronary event during follow-up (n=382) had lower levels at baseline of IgM autoantibodies recognizing the native and malondialdehyde-modified apolipoprotein B-100 peptides p45 and p210 and also lower IgG levels recognizing native p210, whereas no association was found with risk for stroke (n=317). Subjects in the highest compared with lowest tertile of IgM-p45MDA (hazard ratio [95% confidence interval]: 0.72 [0.55, 0.94]; P=0.017) and IgG-p210native (hazard ratio [95% confidence interval]: 0.73 [0.56, 0.97]; P=0.029) had lower risk for incident coronary events after adjustment for cardiovascular risk factors in Cox proportional hazard regression models. Moreover, subjects with high levels of IgG-p210native were less likely to have carotid plaques as assessed by ultrasonography at baseline (odds ratio=0.81, 95% confidence interval 0.70-0.95, P=0.008 after adjustment for risk factors). CONCLUSIONS: This large prospective study demonstrates that subjects with high levels of apolipoprotein B-100 autoantibodies have a lower risk of coronary events supporting a protective role of these autoantibodies in cardiovascular disease.
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