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| 2. |
- Abdel-Rehim, Mohamed, et al.
(författare)
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Microextraction in Packed Syringe Online with Liquid Chromatography-Tandem Mass Spectrometry : Molecularly imprinted polymer as packing material for MEPS in selective extraction of ropivacaine from plasma
- 2006
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Ingår i: Journal of Liquid Chromatography & Related Technologies. - : Informa UK Limited. - 1082-6076 .- 1520-572X. ; 29:12, s. 1725-1736
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Tidskriftsartikel (refereegranskat)abstract
- The excellent performance of a new sample preparation method, microextraction in packed syringe (MEPS), was recently illustrated by online LC‐MS and GS‐MS assays of local anaesthetics in plasma samples. In the method, approximately 1 mg of solid packing material was inserted into a syringe (100–250 µL) as a plug. Sample preparation took place on the packed bed. The new method was easy to use, fully automated, of low cost, and rapid in comparison with previously used methods. This paper presents the use of molecularly imprinted polymers (MIPs) as packing material for higher extraction selectivity. Development and validation of a method for MIP‐MEPS online with LC‐MS‐MS using ropivacaine in plasma as model compound were investigated. A bupivacaine imprinted polymer was used. The method was validated and the standard curves were evaluated by means of quadratic regression and weighted by inverse of the concentration: 1/x for the calibration range 2–2000 nM. The applied polymer could be used more than 100 times before the syringe was discarded. The extraction recovery was 60%. The results showed high correlation coefficients (R 2 >0.999) for all runs. The accuracy, given as a percentage deviation from the nominal concentration values, ranged from -6% to 3%. The precision, given as the relative standard deviation, at three different concentrations (QC samples) was consistently about 3% to 10%. The limit of quantification was 2 nM.
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| 4. |
- Andersson, L. I., et al.
(författare)
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10 Gb/s Optical Transmission using a Silicon Bipolar Chipset
- 1995
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Ingår i: IEEE/LEOS 1995 Digest of the LEOS Summer Topical Meetings. Flat Panel Display Technology. ; , s. 59-60
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Konferensbidrag (refereegranskat)abstract
- A 10 Gb/s silicon chip set including a 8:1 MUX, a 1.8 DMUX, a dynamic divider, and circuits for clock recovery is described. Results from optical transmission experiments utilizing dispersion compensation techniques such as prechirp and MSSI, and bridging distances from 50 to 100 km, will be presented.
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| 5. |
- Andersson, L I, et al.
(författare)
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Immunoassays using molecularly imprinted polymers
- 1995
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Ingår i: Immunoanalysis of agrochemicals. - : American Chemical Society (ACS). - 0841231494 ; , s. 89-97
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 8. |
- Brunius, Carl, 1974, et al.
(författare)
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Prediction and modeling of pre-analytical sampling errors as a strategy to improve plasma NMR metabolomics data
- 2017
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Ingår i: Bioinformatics. - : Oxford University Press (OUP). - 1367-4803 .- 1460-2059 .- 1367-4811. ; 33:22, s. 3567-3574
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Tidskriftsartikel (refereegranskat)abstract
- Biobanks are important infrastructures for life science research. Optimal sample handling regarding e.g. collection and processing of biological samples is highly complex, with many variables that could alter sample integrity and even more complex when considering multiple study centers or using legacy samples with limited documentation on sample management. Novel means to understand and take into account such variability would enable high-quality research on archived samples. This study investigated whether pre-analytical sample variability could be predicted and reduced by modeling alterations in the plasma metabolome, measured by NMR, as a function of pre-centrifugation conditions (1-36 h pre-centrifugation delay time at 4 A degrees C and 22 A degrees C) in 16 individuals. Pre-centrifugation temperature and delay times were predicted using random forest modeling and performance was validated on independent samples. Alterations in the metabolome were modeled at each temperature using a cluster-based approach, revealing reproducible effects of delay time on energy metabolism intermediates at both temperatures, but more pronounced at 22 A degrees C. Moreover, pre-centrifugation delay at 4 A degrees C resulted in large, specific variability at 3 h, predominantly of lipids. Pre-analytical sample handling error correction resulted in significant improvement of data quality, particularly at 22 A degrees C. This approach offers the possibility to predict pre-centrifugation delay temperature and time in biobanked samples before use in costly downstream applications. Moreover, the results suggest potential to decrease the impact of undesired, delay-induced variability. However, these findings need to be validated in multiple, large sample sets and with analytical techniques covering a wider range of the metabolome, such as LC-MS.
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| 9. |
- Chinnasamy, Thiruppathiraja, et al.
(författare)
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Towards paper-based point of care affinity proteomics
- 2014
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Ingår i: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. - : Chemical and Biological Microsystems Society. - 9780979806476 ; , s. 64-66
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Konferensbidrag (refereegranskat)abstract
- Affinity-based methods such as protein microarrays have come to complement conventional mass/charge-based techniques for proteomic characterization of biological samples. Simultaneous measurement of hundreds or even thousands of serum biomarkers such as antibodies, antigens and other proteins may greatly improve the diagnostic accuracy in a variety of conditions such as autoimmune disorders, infections and several cancers. However, today's technologies for affinity proteomics are cumbersome, require expensive equipment and skilled operators. Here, we present two novel paper-based techniques developed in our lab that aim to bridge the gap between highly multiplexed affinity proteomics and point of care testing.
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