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- Itoh, Yuzuru, et al.
(författare)
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Mechanism of membrane-tethered mitochondrial protein synthesis
- 2021
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 371:6531, s. 846-849
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Tidskriftsartikel (refereegranskat)abstract
- Mitochondrial ribosomes (mitoribosomes) are tethered to the mitochondrial inner membrane to facilitate the cotranslational membrane insertion of the synthesized proteins. We report cryo-electron microscopy structures of human mitoribosomes with nascent polypeptide, bound to the insertase oxidase assembly 1-like (OXA1L) through three distinct contact sites. OXA1L binding is correlated with a series of conformational changes in the mitoribosomal large subunit that catalyze the delivery of newly synthesized polypeptides. The mechanism relies on the folding of mL45 inside the exit tunnel, forming two specific constriction sites that would limit helix formation of the nascent chain. A gap is formed between the exit and the membrane, making the newly synthesized proteins accessible. Our data elucidate the basis by which mitoribosomes interact with the OXA1L insertase to couple protein synthesis and membrane delivery.
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| 3. |
- Peilot, Birgitta, 1947, et al.
(författare)
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Evaluation of treatment with Cognitive therapy and a patient-centered approach in patients with chronic musculoskeletal pain and psychiatric co-morbidity – A pilot study
- 2019
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Ingår i: ECRONICON open access. ; 11:8, s. 612-629
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Tidskriftsartikel (refereegranskat)abstract
- Objective. The aim was to evaluate Cognitive therapy (CT) and a patient-centered-approach in a group of patients with chronic pain and psychiatric co-morbidity in relation to pain, dysfunction, anxiety, depression, burnout and health related quality of life (HRQoL). Design. Pilot study. Setting. Consulting psychiatry. Participants 30 consecutive patients with chronic pain, who had not responded to conventional pain treatment, were recruited during a period of 21 months. Interventions. All patients were offered treatment with cognitive therapy (CT). Twenty patients accepted treatment with CT and 13 patients, the therapy group, completed therapy. The no therapy group consisted of 7 patients who did not complete therapy and 10 patients who were only followed-up at monthly intervals. Main otutcome measures. Patient reported outcome measures (PROM) were administered at baseline and at follow-up after 8-11 months. PROM included Relationship Scales Questionnaire (RSQ ) for attachment pattern, Sense of coherence (SOC), Multidimensional Pain Inventory (MPI) for pain and dysfunction, Beck Anxiety Inventory (BAI), Beck Depression Inventory (BDI), Pines´scale for burnout and Short-Form 36 (SF-36). Statistical tests were conducted at a 5% significance level. Subgroup identification was accomplished by means of Multivariate data analysis (MVDA). Results. At the individualized follow-up the patients (n=25) reported significant improvement of Bodily Pain and Mental Health in Short-Form 36 ( SF-36), life control in MPI, depression, anxiety and SOC. There was a correlation between completed treatment with CT and improvement. Explorative modellings with MVDA suggested that patients with chronic pain and fearful attachment pattern had a better outcome than patients with preoccupied attachment pattern if they were treated with CT. Conclusion.. A patient-centered approach in combination with CT was beneficial for patients with chronic pain and psychiatric co-morbidity. MVDA indicated that the outcome of treatment may differ according to individual vulnerability and severity of burnout, depression and pain interference. Strengths and limitations of the study * This study is a small hypothesis generating pilot study and the results need to be followed up with larger randomized studies. *Patients who dropped out from therapy were followed in the no therapy group * The same therapist/physician (BP) treated the entire group. * In order to compensate for many variables and a limited number of observations explorative modellings with MVDA were performed.
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| 4. |
- Peilot, Birgitta, 1947, et al.
(författare)
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Time to gain trust and change-Experiences of attachment and mindfulness-based cognitive therapy among patients with chronic pain and psychiatric co-morbidity
- 2014
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Ingår i: International Journal of Qualitative Studies on Health and Well-Being. - : Informa UK Limited. - 1748-2623 .- 1748-2631. ; 9:24420
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Tidskriftsartikel (refereegranskat)abstract
- The treatment of patients with chronic pain disorders is complex. In the rehabilitation of these patients, coping with chronic pain is seen as important. The aim of this study was to explore the meaning of attachment and mindfulness-based cognitive therapy (CT) among patients with chronic pain and psychiatric co-morbidity. A phenomenological approach within a lifeworld perspective was used. In total, 10 patients were interviewed after completion of 7-to 13-month therapy. The findings reveal that the therapy and the process of interaction with the therapist were meaningful for the patients' well-being and for a better management of pain. During the therapy, the patients were able to initiate a movement of change. Thus, CT with focus on attachment and mindfulness seems to be of value for these patients. The therapy used in this study was adjusted to the patients' special needs, and a trained psychotherapist with a special knowledge of patients with chronic pain might be required.
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| 5. |
- Tin, Amy L., et al.
(författare)
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Pain as bad as you can imagine or extremely severe pain? A randomized controlled trial comparing two pain scale anchors
- 2023
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Ingår i: Journal of Patient-Reported Outcomes. - 2509-8020. ; 7:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: A common method of pain assessment is the numerical rating scale, where patients are asked to rate their pain on a scale from 0 to 10, where 0 is “no pain” and 10 is “pain as bad as you can imagine”. We hypothesize such language is suboptimal as it involves a test of a cognitive skill, imagination, in the assessment of symptom severity. Methods: We used a large-scale online research registry, ResearchMatch, to conduct a randomized controlled trial to compare the distributions of pain scores of two different pain scale anchors. We recruited adults located in the United States who reported a chronic pain problem (> 3 months) and were currently in pain. Participants were randomized in a 1:1 ratio to receive pain assessment based on a modified Brief Pain Inventory (BPI), where the anchor for a score of 10 was either “extremely severe pain”, or the original BPI, with the anchor “pain as bad as you can imagine”. Participants in both groups also answered additional questions about pain, other symptomatology and creativity. Results: Data were obtained from 405 participants for the modified and 424 for the original BPI. Distribution of responses to pain questions were similar between groups (all p-values ≥ 0.12). We did not see evidence that the relationship between pain score and the anchor text differed based on self-perceived creativity (all interaction p-values ≥ 0.2). However, in the key analysis, correlations between current pain assessments and known correlates (fatigue, anxiety, depression, current pain compared to a typical day, pain compared to other people) were stronger for “extreme” vs. “imaginable” anchor text (p = 0.005). Conclusion: Pain rating scales should utilize the modified anchor text “extremely severe pain” instead of “pain as bad as you can imagine”. Further research should explore the effects of anchors for other symptoms.
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