| 1. |
- Anesio, Alexandre Magno, et al.
(författare)
-
Bacterial growth on photochemically transformed leachates from aquatic and terrestrial primary producers
- 2000
-
Ingår i: Microbial Ecology. - : Springer Science and Business Media LLC. - 1432-184X .- 0095-3628. ; 40:3, s. 200-208
-
Tidskriftsartikel (refereegranskat)abstract
- We measured bacterial growth on phototransformed dissolved organic matter (DOM) leached from eight different primary producers. Leachates (10 mg C liter(-1)) were exposed to artificial UVA + UVB radiation, or kept in darkness, for 20 h. DOM solutions were subsequently inoculated with lake water bacteria. Photoproduction of dissolved inorganic carbon (DIC), ranging from 3 to 16 mug C liter(-1) h(-1), and changes in the absorptive characteristics of the DOM were observed for all leachates upon UV irradiation. The effects of irradiation exposure on DOM bioavailability varied greatly, depending on leachate and type of bacterial growth criterion. Bacterial carbon utilization (biomass production plus respiration) over the entire incubation period (120 h) was enhanced by UV radiation of leachate from the terrestrial leaves, relative to carbon utilization in non-irradiated leachates. Conversely, carbon utilization was reduced by radiation of the leachates from aquatic macrophytes. In a separate experiment, the stable C and N isotope composition of bacteria grown on irradiated and non-irradiated DOM was estimated. Bacterial growth on UV-irradiated DOM was enriched in C-13 relative to the bacteria in the non-irradiated treatments; this result may be explained by selective assimilation of photochemically produced, isotopically enriched labile compounds.
|
|
| 2. |
- Anesio, Alexandre Magno, et al.
(författare)
-
Effect of Humic Substance Photodegradation on Bacterial Growth and Respiration in Lake Water
- 2005
-
Ingår i: Applied and Environmental Microbiology. - 0099-2240. ; 71:10, s. 6267-6275
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- This study addresses how humic substance (HS) chemical composition and photoreactivity affect bacterial growth, respiration, and growth efficiency (BGE) in lake water. Aqueous solutions of HSs from diverse aquatic environments representing different dissolved organic matter sources (autochthonous and allochthonous) were exposed to artificial solar UV radiation. These solutions were added to lake water passed through a 0.7-µm-pore-size filter (containing grazer-free lake bacteria) followed by dark incubation for 5, 43, and 65 h. For the 5-h incubation, several irradiated HSs inhibited bacterial carbon production (BCP) and this inhibition was highly correlated with H2O2 photoproduction. The H2O2 decayed in the dark, and after 43 h, nearly all irradiated HSs enhanced BCP (average 39% increase relative to nonirradiated controls, standard error = 7.5%, n = 16). UV exposure of HSs also increased bacterial respiration (by 18%, standard error = 5%, n = 4), but less than BCP, resulting in an average increase in BGE of 32% (standard error = 10%, n = 4). Photoenhancement of BCP did not correlate to HS bulk properties (i.e., elemental and chemical composition). However, when the photoenhancement of BCP was normalized to absorbance, several trends with HS origin and extraction method emerged. Absorbance-normalized hydrophilic acid and humic acid samples showed greater enhancement of BCP than hydrophobic acid and fulvic acid samples. Furthermore, absorbance-normalized autochthonous samples showed 10-fold greater enhancement of BCP than allochthonous-dominated samples, indicating that the former are more efficient photoproducers of biological substrates.
|
|
| 3. |
- Anesio, Alexandre Magno
(författare)
-
Effects of solar radiation on the abiotic and bacterially mediated carbon flux in aquatic ecosystems
- 2000
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In this Thesis, I studied some of the current aspects of organic matter photochemistry. I analyzed abiotic phototransformations of several types of dissolved (DOM) and particulate organic matter (POM). I also evaluated the effects of phototransformation of several types of DOM on bacteria. Finally, in a field experiment, I analyzed net effects of solar radiation on organic matter decomposition. DOM undergoes several transformations due to solar irradiation. One such transformation is photooxidation of organic matter into inorganic carbon. Results of this Thesis show that photooxidation is ubiquitous to all kinds of organic matter in both dissolved and particulate forms. The intensity of this process depends on several factors, including DOM composition, radiation type and time of exposure. Besides mineralization to inorganic carbon, DOM undergoes other chemical transformations due to UV radiation, with profound consequences to DOM availability for bacteria. Bioavailability was tested by measuring bacterial growth and respiration on irradiated and non-irradiated DOM from several types of humic matter and plant leachates. Irradiation of freshly-leached DOM often produced negative effects on bacteria, whereas irradiation of humic material was followed by stimulation of bacterial growth. The degree of stimulation seems to be related to the initial bioavailability of the DOM and to the capability of the DOM to produce hydrogen peroxide upon irradiation. Other factors also accounted for differences in bacterial response to photochemical modification of DOM, including length and type of irradiation exposure. The effects of solar radiation on litter decomposition were also evaluated using experiments that more closely mimic natural conditions. I could not observe differences between dry weight loss of leaves and culms exposed to solar radiation or kept in darkness, which may be explained by the fact that abiotic decomposition under solar radiation is counterbalanced by negative effects of solar radiation on decomposers.
|
|
| 4. |
- Anesio, Alexandre Magno, et al.
(författare)
-
Increased photoreactivity of DOC by acidification: Implications for the carbon cycle in humic lakes
- 2003
-
Ingår i: Limnology and Oceanography. - 1939-5590. ; 48:2, s. 735-744
-
Tidskriftsartikel (refereegranskat)abstract
- Effects of ultraviolet (UV)-B radiation and acidification on pelagic carbon flux in a humic lake (dissolved organic carbon [DOC] similar to15 mg C L-1) were studied in a mesocosm experiment during the summer of 2000. Triplicate tanks (107 liters volume, 1 m high) were exposed to natural solar radiation, a daily extra dose of UV-B radiation, or kept dark. One set of tanks was submitted to a decrease in pH (from 5.7 to 4.7), and one set was kept at the natural pH level. During 70 d, water samples were taken regularly from the mesocosms for measurements of DOC, absorbance, dissolved inorganic carbon (DIC), and pH. Additionally, we regularly incubated samples to measure photooxidation rates, primary production, and community respiration. We found an increase in the photooxidation rates in the acidified mesocosms relative to ambient pH. The greater abiotic production of DIC (i.e., photooxidation) in acidified conditions could explain similar to27% of the decline in DOC in the same conditions. Laboratory experiments were done to test the effects of pH on the dissolved organic matter (DOM) photoreactivity. At lower pH values, we found both higher abiotic DIC production and specific absorbance fading, relative to neutral pH values in water from a humic lake. In a separate experiment, samples were exposed to prolonged irradiation under laboratory conditions, resulting in complete loss of absorptivity in the wavelengths between 290 and 400 nm. Decreases in DOC in the long-term exposure caused by photochemical mineralization were similar to45 and 55% of the initial pool for natural pH and acidified samples, respectively, at the end of the experiment. An increase in the dissolved organic matter photoreactivity by acidification could be an important mechanism to explain the increased water transparency and in-lake DOC removal in acid lakes found in several previous studies.
|
|
| 5. |
- Anesio, Alexandre Magno, et al.
(författare)
-
Influence of humic substances on bacterial and viral dynamics in freshwaters
- 2004
-
Ingår i: Applied and Environmental Microbiology. - 0099-2240. ; 70:8, s. 4848-4854
-
Tidskriftsartikel (refereegranskat)abstract
- Bacterial and viral abundances were measured in 24 lakes with dissolved organic carbon (DOC) concentrations ranging from 3 to 19 mg of C liter(-1). In addition, a laboratory experiment was performed to test the effects of different sources of carbon (i.e., glucose and fulvic acids) and nutrients on the dynamics of viruses and bacteria. In the lake survey, no correlation was found between virus abundance and DOC concentration, yet there was a significant positive correlation between bacterial abundance and DOC concentration. A negative correlation was found between the virus-to-bacteria ratio and DOC level. These results are in agreement with our findings in the laboratory, where virus counts were significantly lower in treatments with fulvic acid additions than in a control (mean, 67.4% +/- 6.5% of the control). Virus counts did not differ significantly among the control and treatments with glucose, indicating that it was the type of organic carbon and not quantity which had an impact on viruses. Results from this study suggest that the way viruses control bacterial assemblages in humic lakes is different from the mechanism in clear water systems.
|
|
| 6. |
- Anesio, Alexandre Magno, et al.
(författare)
-
Photochemical mineralization of dissolved organic carbon in lakes of differing pH and humic content
- 2004
-
Ingår i: Archiv für Hydrobiologie. - : Schweizerbart. - 0003-9136. ; 160:1, s. 105-116
-
Tidskriftsartikel (refereegranskat)abstract
- Photochemical mineralization of dissolved organic carbon (DOC) to dissolved inorganic carbon (DIC) was studied in surface water from 10 oligotrophic lakes in Southern Sweden differing in DOC, pH, and iron content. In addition, we studied the effects of acidification on the DOC photoreactivity. Samples from each lake were filtered through 0.2 mum-filters, acidified by one pH unit or kept under ambient pH conditions, and incubated in quartz tubes, in situ and in the laboratory. We found a positive relationship between photochemical DIC production at the surface and DOC concentration or absorbed dose of UV radiation. On average, lake water treated with acid showed higher rates of photooxidation than lake water with ambient pH. However, the difference in rates of photooxidation between acidified and non-acidified treatments was only evident for lake water with high DOC and iron content (i.e. lakes with DOC concentration > 10 mg C/l, and iron concentration > 400 mug/l). Results from this study indicate that acidification may increase photochemical mineralization, especially in humic lakes. Finally, we showed that a substantial increase in UV-B radiation (e. g. a doubling) would influence the rates of photochemical mineralization of DOC in humic lakes (DOC > 10 mg C/l) substantially less than a rather moderate decrease in pH of one unit. In clear lakes an increased UV-B radiation would have a stronger impact on areal DIC-production than a decrease in pH.
|
|
| 7. |
- Anesio, Alexandre Magno, et al.
(författare)
-
The role of free and attached microorganisms in the decomposition of estuarine macrophyte detritus
- 2003
-
Ingår i: Estuarine, Coastal and Shelf Science. - 1096-0015. ; 56:2, s. 197-201
-
Tidskriftsartikel (refereegranskat)abstract
- Abundance and respiration of free and attached microorganisms were monitored during the decomposition of the seagrass Scirpus maritimus leaves in laboratory microcosms for 30 days. There was a clear succession between bacteria and heterotrophic flagellates during the course of the study. The beginning of the study (1-4 days) was characterized by higher rates of bacterial respiration, compared to the later periods. Free microorganisms were responsible for more than half of the respiration (65%) within the microcosms, suggesting that they were responsible for the mineralization of the bulk of the macrophyte detritus following its dissolution. On the other hand, estimates of activity on a per cell basis revealed that individual attached bacteria had much higher (3- to 4-fold) respiration rates than free bacteria, suggesting attached bacterial activity may play a key role in the breakdown and dissolution of particulate detritus in estuarine waters. The findings suggest different but coupled roles for attached and free bacteria in nature.
|
|
| 8. |
- Daniel, Cesar Bolivar, et al.
(författare)
-
Stimulation of metazooplankton by photochemically modified dissolved organic matter
- 2006
-
Ingår i: Limnology and Oceanography. - 1939-5590. ; 51:1, s. 101-108
-
Tidskriftsartikel (refereegranskat)abstract
- We examined the response of bacteria and proto- and metazooplankton to photomodified dissolved organic matter (DOM). Sterile filtered water from a eutrophic and a humic lake, that was either exposed to artificial ultraviolet (UV) radiation or kept dark, was added to semicontinuous laboratory microcosms that lasted for 7 weeks. Bacterial production responded positively to photochemical modification of DOM regardless of lake type. Final heterotrophic biomass (bacteria + proto + metazooplankton) was 47 +/- 5 and 37 +/- 5 mu g carbon (C) L-1 in microcosms with UV-exposed and unaltered eutrophic water DOM and 15 +/- 4 and 11 +/- 2 mu g C L-1 in microcosms with UV-exposed and unaltered humic water DOM, respectively. For the eutrophic water, there were no significant differences in proto- or metazooplankton biomasses between microcosms receiving UV-exposed or nonexposed DOM. Differences between eutrophic water microcosms were not significant when flagellates, ciliates, cladocerans, and copepods were examined separately. In microcosms with UV-exposed humic water, biomasses of heterotrophic flagellates, rotifers, nauplii, and cladocerans were higher than in those with nonexposed DOM. Higher final metazooplankton biomass following addition of UV-exposed humic water indicates that photochemically modified DOM can be effectively transferred through the microbial loop.
|
|
| 9. |
- Daniel, C, et al.
(författare)
-
Microbial food webs in the dark: independence of Lake Plankton from recent algal production
- 2005
-
Ingår i: Aquatic Microbial Ecology. - 0948-3055. ; 38:2, s. 113-123
-
Tidskriftsartikel (refereegranskat)abstract
- We investigated the development of a heterotrophic plankton food web with or without phytoplankton primary production in a long-term (>1 yr) laboratory experiment. Water from 3 Swedish lakes (humic, oligotrophic clearwater, eutrophic) was exposed to low light or kept in total darkness in triplicate 100 1 cylinders. Dissolved organic carbon (DOC) dynamics, bacterial growth and biomass of protozoans, rotifers and microcrustaceans were followed over 18 mo. In the dark treatments, no primary production was detected and DOC concentrations decreased by between 19 and 27% (1.3 to 3.2 mg C l(-1)). There was bacterial and protozoan growth in the dark during the whole experimental period. However, numbers and production of bacteria, as well as protozoan biomass, were significantly lower in darkness. Dissolved (DOM) and particulate organic matter (POM) initially present in the water (i.e. 18 mo old at the end of the experiment) helped to support substantial metazoan biomasses in dark treatments in the humic and eutrophic waters, but not in the oligotrophic clearwater lake. DOM in the humic water, thus largely of allochthonous origin, gave the highest and most prolonged support to zooplankton living in darkness. Our study indicates that a microbial food web, based on allochthonous organic matter and developing independently from phytoplankton, can act as a link to metazoan zooplankton, especially in oligotrophic humic lakes. These results confirm studies using stable C isotopes, showing a substantial incorporation of terrestrial carbon into zooplankton.
|
|
| 10. |
- Denward, C M T, et al.
(författare)
-
Solar radiation effects on decomposition of macrophyte litter in a lake littoral
- 2001
-
Ingår i: Archiv für Hydrobiologie. - 0003-9136. ; 152:1, s. 69-80
-
Tidskriftsartikel (refereegranskat)abstract
- Ambient solar radiation effects on Phragmites australis leaf and culm litter were investigated in a shallow and eutrophic south Swedish lake littoral. Leaf and culm litter was exposed to natural sunlight in the lake at a water depth of a few centimeters. For the leaf litter, an additional subset of the experiment was exposed to solar radiation in the air, to evaluate effects of the solar radiation on the leaf litter in a dry state. Radiation treatments (shaded, photosynthetic active radiation [PAR], PAR + ultraviolet-A [UVA] and PAR + UVA + ultraviolet-B [UVB]) were achieved by screening with Plexiglas and Mylar film. Decomposition was measured as dry weight loss, and fungal and bacterial abundance on the detritus was estimated as ergosterol and bacterial numbers, respectively. We found no differences in either weight loss or bacterial abundance among radiation treatments. The fungal biomass in the dry leaf litter was unaffected by the radiation. In the wet leaf litter, however, the ergosterol content in PAR, PAR + UVA and PAR + UVA + UVB treated samples was about one third of the amounts found in the initial material and in the samples kept darkened. Similarly, the fungal biomass associated with the culm litter was negatively affected by solar PAR + UVA + UVB radiation, but in culms exposed only to PAR or to PAR + UVA it was not significantly different from the fungal biomass in darkened samples. These results suggest that the net effects of radiation differ between fungi and bacteria, with the fungi being more susceptible to suppression by solar radiation than the bacteria. Our experiments mimic more closely than previously published studies the conditions that can be expected in natural environments. Hence, we propose that previous reports of strong radiation effects on aquatic liner degradation should be applied very carefully to natural conditions.
|
|
