| 1. |
- Angelov, Borislav, et al.
(författare)
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An integrated model of technical system design : Further development of the concept
- 2021
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Ingår i: AIP Conference Proceedings. - : American Institute of Physics (AIP). - 9780735441408
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Konferensbidrag (refereegranskat)abstract
- As a result of the research performed earlier, it was found that the development of technical systems in practice solves four major tasks-adapting an existing solution to work in new conditions, refining an existing solution, combining it with previously developed elements and creating a new solution. Accordingly, the principal focus of the present research is on a more integrated model for the design of technical systems. The model is obtained by combining (overlaying) the methods for solving the four main tasks defined. The current study presents the findings from the subsequent development of the concept-the place of the scientific research in the proposed integrated model is determined and the process of selecting or synthesizing a technical proposal in each of the defined four cases is clarified.
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| 2. |
- Angelov, Borislav, et al.
(författare)
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The system for the conversion of matter, energy and information : the missing element in the theory of technical systems
- 2012
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Ingår i: The Seventh International Symposium KOD 2012 Machine and Industrial Design in Mechanical Engineering Proceedings. - Novi Sad : Faculty of Technical Sciences. - 9788678923999 ; , s. 65-70
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Konferensbidrag (refereegranskat)abstract
- The last years of the past century were characterized by the rapid development of engineering. Technical sciences have also made their evolutionary leap. What is still missing is a generally accepted scientific subject that systematizes the existing knowledge in one scientific complex, based on the general principles in the structure, design and use of the technical systems. The paper presents results from the analysis of the object of the study and the contents of a general science of the technical systems.
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| 3. |
- Angelov, Borislav, et al.
(författare)
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Topology and internal structure of PEGylated lipid nanocarriers for neuronal transfection : synchrotron radiation SAXS and cryo-TEM studies
- 2011
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Ingår i: Soft Matter. - : Royal Society of Chemistry (RSC). - 1744-683X .- 1744-6848. ; 7:20, s. 9714-9720
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Tidskriftsartikel (refereegranskat)abstract
- Towards potential treatment of neurodegenerative disorders by nanomedicine gene therapy, a nonviral gene delivery system is created by complexation of novel PEGylated cationic lipid assemblies and a plasmid DNA encoding for the protein brain-derived neurotrophic factor (BDNF). The generated monoolein-based liquid crystalline nanocarriers for BDNF gene transfer are characterized by means of small-angle X-ray scattering (SAXS) and cryogenic transmission electron microscopy (cryo-TEM) techniques. The complexation of the BDNF plasmids (pBDNF) to the positively charged lipid nanocarriers, is directly detected by turbidity measurements. Morphologically variable populations of sterically stabilized, PEGylated multicompartment nanoobjects, with confined supercoiled DNA, are established by cryo-TEM imaging. Synchrotron radiation SAXS measurements, performed with diluted nanoparticulate dispersions, evidence the inner multilamelar structure of pear-type vesicles and flexible onion-like objects functionalized by pBDNF. The experimental SAXS intensity curves are fitted with a scattering model for multilamellar vesicles involving five lamellas. As the monoolein-based multicompartment nanocarriers can strongly affect the organization of targeted membranes, the obtained structural data are of fundamental interest for improvement of brain-targeted gene delivery and understanding of cellular internalization mechanisms of nanocarriers in neuronal transfection studies.
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| 4. |
- Haralanova, Valentina, et al.
(författare)
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Generalized algorithm for a functional : structural analysis of technical systems
- 2012
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Ingår i: The Seventh International Symposium KOD 2012 Machine and Industrial Design in Mechanical Engineering PROCEEDINGS. - Novi Sad. - 9788678923999 ; , s. 71-74
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Konferensbidrag (refereegranskat)abstract
- A generalized algorithm for functionalstructural analysis of the technical systems based on the hierarchical relationship between the system for conversion of material, energy and information, the technical system and the executive organs of the technical system, is proposed. The universality of the proposed algorithm is a prerequisite for solving important problems in the theory of the technical systems for revealing regularities, generally valid for all technical objects and transferring knowledge from one scientific field to another one.
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| 5. |
- Khakurel, Krishna P., et al.
(författare)
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Macromolecular nanocrystal structural analysis with electron and X-rays: A comparative review
- 2019
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Ingår i: Molecules. - : MDPI AG. - 1420-3049 .- 1420-3049. ; 24:19
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Forskningsöversikt (refereegranskat)abstract
- Crystallography has long been the unrivaled method that can provide the atomistic structural models of macromolecules, using either X-rays or electrons as probes. The methodology has gone through several revolutionary periods, driven by the development of new sources, detectors, and other instrumentation. Novel sources of both X-ray and electrons are constantly emerging. The increase in brightness of these sources, complemented by the advanced detection techniques, has relaxed the traditionally strict need for large, high quality, crystals. Recent reports suggest high-quality diffraction datasets from crystals as small as a few hundreds of nanometers can be routinely obtained. This has resulted in the genesis of a new field of macromolecular nanocrystal crystallography. Here we will make a brief comparative review of this growing field focusing on the use of X-rays and electrons sources.
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| 6. |
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| 7. |
- Mai, Dong Du, et al.
(författare)
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Laser-Driven Photon Sources and Exemplary Applications at ELI Beamlines
- 2022
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Ingår i: Optics InfoBase Conference Papers. - 9781957171050
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Konferensbidrag (refereegranskat)abstract
- E(xtreme)L(ight)I(nfrastructure) Beamlines within the EU's ELI project provides laser-driven secondary sources of EUV/XUV, X-ray and relativistic charged particles offering unique pump/probe/harvest experimental schemes carried out by various end stations.
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| 8. |
- Polovinkin, Vitaly, et al.
(författare)
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Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures
- 2020
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Ingår i: Journal of applied crystallography. - 0021-8898 .- 1600-5767. ; 53, s. 1416-1424
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Tidskriftsartikel (refereegranskat)abstract
- Electron crystallography of sub-micrometre-sized 3D protein crystals has emerged recently as a valuable field of structural biology. In meso crystallization methods, utilizing lipidic mesophases, particularly lipidic cubic phases (LCPs), can produce high-quality 3D crystals of membrane proteins (MPs). A major step towards realizing 3D electron crystallography of MP crystals, grown in meso, is to demonstrate electron diffraction from such crystals. The first task is to remove the viscous and sticky lipidic matrix that surrounds the crystals without damaging the crystals. Additionally, the crystals have to be thin enough to let electrons traverse them without significant multiple scattering. In the present work, the concept that focused ion beam milling at cryogenic temperatures (cryo-FIB milling) can be used to remove excess host lipidic mesophase matrix is experimentally verified, and then the crystals are thinned to a thickness suitable for electron diffraction. In this study, bacteriorhodopsin (BR) crystals grown in a lipidic cubic mesophase of monoolein were used as a model system. LCP from a part of a hexagon-shaped plate-like BR crystal (∼10 µm in thickness and ∼70 µm in the longest dimension), which was flash-frozen in liquid nitrogen, was milled away with a gallium FIB under cryogenic conditions, and a part of the crystal itself was thinned into a ∼210 nm-thick lamella with the ion beam. The frozen sample was then transferred into an electron cryo-microscope, and a nanovolume of ∼1400 × 1400 × 210 nm of the BR lamella was exposed to 200 kV electrons at a fluence of ∼0.06 e Å−2. The resulting electron diffraction peaks were detected beyond 2.7 Å resolution (with an average peak height to background ratio of >2) by a CMOS-based Ceta 16M camera. The results demonstrate that cryo-FIB milling produces high-quality lamellae from crystals grown in lipidic mesophases and pave the way for 3D electron crystallography on crystals grown or embedded in highly viscous media.
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