| 1. |
- Hedman, Johannes, et al.
(författare)
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A fast analysis system for forensic DNA reference samples
- 2008
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Ingår i: Forensic Science International: Genetics. - : Elsevier BV. - 1878-0326 .- 1872-4973. ; 2:3, s. 184-189
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Tidskriftsartikel (refereegranskat)abstract
- On January 1st, 2006, the Swedish legislation on obtaining DNA reference samples from Suspects and the recording of DNA profiles in databases was changed. As a result the number of samples analysed at the Swedish National Laboratory of Forensic Science (SKL) increased from about 4500 in 2005 to more than 25,000 in 2006. To meet this challenge, SKL launched a flew analysis system to create an unbroken chain, from sampling to incorporation of a profile in the national DNA database and subsequent automatic generation of digitally signed hit reports. The system integrates logistics, digital data transfer, new functions in LIMS (ForumDNA Version 4, Ida Infront AB) and laboratory automation. Buccal swab samples are secured on a FTA (R) card attached to an identity form, which is barcoded with a unique sample ID. After sampling, the police officer sends a digital request to SKL. The sample is automatically registered in LIMS and processed on delivery. The resulting DNA profiles are automatically classified according to quality using a custom-made expert system. Building the evaluation around mathematical rules makes it reproducible, standardised and minimises manual work and clerk errors. All samples are run in duplicate and the two profiles are compared within LIMS before incorporation in the database. In the first year of operation, the median time for completion of an analysis was 3 days, measured from delivery of the sample to incorporation of the profile in the national DNA database. In spite of the dramatic increase in the number of reference samples there was no backlog. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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| 2. |
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| 3. |
- Ansell, Ricky, 1967-, et al.
(författare)
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Läkares säkring av bevis efter sexualbrott viktig del i rättsprocessen
- 2008
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Ingår i: Läkartidningen. - 0023-7205 .- 1652-7518. ; 105:9, s. 634-637
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Tidskriftsartikel (refereegranskat)abstract
- In cases of sexual assault, physical evidence can be of crucial importance for a conviction. Intimate samples initially collected by a physician can prove to be the only supporting evidence for the prosecution to present at court proceedings. New analysis techniques and methods have increased the positive outcome of the samples collected. This in combination with increased use of national criminal DNA databases results in the solving of sexual crimes with unknown perpetrators. The use of standardised rape care kits facilitates the work of the physician in performing an adequate sampling procedure. The Swedish "rape care kit" has been developed and updated in response to experience gained and new possibilities.
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| 4. |
- Boiso, Samuel, et al.
(författare)
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RapidHIT for the purpose of stain analyses – An interrupted implementation
- 2017
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Ingår i: Forensic Science International. - : Elsevier. - 1875-1768 .- 1875-175X. ; 6:Supplement C, s. e589-e590
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Tidskriftsartikel (refereegranskat)abstract
- Rapid DNA instruments have in recent years been developed, enabling analysis of forensic samples with a minimum of human intervention. Initially intended for fast handling of reference samples, such as samples from suspects in booking suites, attention shifted to include crime scene samples. The aim of this study was to determine whether or not the RapidHIT System (IntegenX) is fit for crime scene samples. The first runs gave very poor results, which was found to be due to an incorrect firmware setting leading to no or just minute amounts of amplicons being injected for electrophoresis. After solving this problem, 28 full runs (seven samples each) applying NGM SElect Express were performed comprising various amounts of blood on cotton swabs. Six of the runs failed completely, four due to cartridge leakage and in two runs the PCR mix was not injected. For 155 samples with 1–5ΌL blood (volumes for which complete DNA profiles are expected), 119 samples (77%) gave complete DNA profiles. Among the most serious failures were incorrect allele calling and leakage of DNA extract or PCR product. Other general issues were failure to export results, anode motor breakdown and broken capillary array. Due to the encountered problems with software, hardware and cartridges, together with the low success rate, it was decided not to continue towards implementation of the RapidHIT System in casework.
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| 5. |
- Dufva, Charlotte, et al.
(författare)
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DNA profiles obtained from urine in snow
- 2019
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Ingår i: Forensic Science International. - : ELSEVIER IRELAND LTD. - 1875-1768 .- 1875-175X. ; 7:1, s. 544-545
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Tidskriftsartikel (refereegranskat)abstract
- Urine can be a potential important source of evidence when occurring at crime scenes. In case of outdoor scenes including snow a yellow colour could indicate the existence of human urine. In an effort to be able to investigate urine as a crime scene sample we have evaluated protocols for analysing DNA from urine in snow. Two different tests were performed with a smaller and a larger volume of urine dispensed on snow. The tubes were put into a freezer to mimic winter conditions. A Urine Preservative was added to some of the samples. DNA profiles were compared between samples extracted using a Urine DNA Isolation Kit and samples extracted with a Chelex-based method. In addition, a test was performed with the aim to mimic a potential crime scene. With a smaller volume of urine the best quality DNA profiles were obtained using the Urine DNA Isolation Kit without the Urine Preservative. When a larger volume of urine was handled and in the crime scene setup, there were no clear difference between the two extraction methods. Instead, the variation observed was between individuals.
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| 6. |
- Hedman, Johannes, et al.
(författare)
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Using the new Phadebas® Forensic Press test to find crime scene saliva stains suitable for DNA analysis
- 2008
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Ingår i: Forensic Science International: Genetics Supplement Series. - : Elsevier BV. - 1875-1768. ; 1:1, s. 430-432
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Tidskriftsartikel (refereegranskat)abstract
- The Phadebas® Forensic Press test is a new product that detects saliva stains by reacting with amylase. When the paper is pressed against a saliva stain a blue spot occurs. To test the sensitivity of the paper, a set of dilution series of saliva down to 1:500 was prepared on cotton fabric. Blue spots could be seen for dilutions of 1:100 when incubated at room temperature, and 1:200 in 37 °C. However, incubation at 37 °C did not provide acceptable reproducibility. The Phadebas® test was compared to four different lightsources for the ability to detect saliva on different carrier materials. Cotton fabric (T-shirt), denim, suede, leather, painted wood and untreated wood were tested. On denim, no stains could be detected with the lightsources, but Phadebas® showed all stains for pure saliva and dilution 1:5. DNA analysis was performed on detected stains and corresponding spots on the Phadebas® paper. Complete DNA profiles were produced for stains from pure saliva and dilution 1:5.
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| 7. |
- Kokshoorn, Bas, et al.
(författare)
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Sharing data on DNA transfer, persistence, prevalence and recovery : Arguments for harmonization and standardization
- 2018
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Ingår i: Forensic Science International. - : Elsevier. - 1872-4973 .- 1878-0326. ; 37, s. 260-269
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Tidskriftsartikel (refereegranskat)abstract
- Sharing data between forensic scientists on DNA transfer, persistence, prevalence and recovery (TPPR) is crucial to advance the understanding of these issues in the criminal justice community. We present the results of a collaborative exercise on reporting forensic genetics findings given activity level propositions. This exercise outlined differences in the methodology that was applied by the participating laboratories, as well as limitations to the use of published data on DNA TPPR. We demonstrate how publication of experimental results in scientific journals can be further improved to allow for an adequate use of these data. Steps that can be taken to share and use these data for research and casework purposes are outlined, and the prospects for future sharing of data through publicly accessible databases are discussed. This paper also explores potential avenues to proceed with implementation and is intended to fuel the discussion on sharing data pertaining to DNA TPPR issues. It is further suggested that international standardization and harmonization on these topics will benefit the forensic DNA community as it has been achieved in the past with the harmonization of STR typing systems.
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| 8. |
- Tuazon, Oliver M., et al.
(författare)
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Law enforcement use of genetic genealogy databases in criminal investigations: Nomenclature, definition and scope
- 2024
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Ingår i: Forensic Science International: Synergy. - : Elsevier. - 2589-871X. ; 8, s. 100460-100460
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Tidskriftsartikel (refereegranskat)abstract
- Although law enforcement use of commercial genetic genealogy databases has gained prominence since the arrest of the Golden State Killer in 2018, and it has been used in hundreds of cases in the United States and more recently in Europe and Australia, it does not have a standard nomenclature and scope. We analyzed the more common terms currently being used and propose a common nomenclature: investigative forensic genetic genealogy (iFGG). We define iFGG as the use by law enforcement of genetic genealogy combined with traditional genealogy to generate suspect investigational leads from forensic samples in criminal investigations. We describe iFGG as a proper subset of forensic genetic genealogy, that is, FGG as applied by law enforcement to criminal investigations; hence, investigative FGG or iFGG. We delineate its steps, compare and contrast it with other investigative techniques involving genetic evidence, and contextualize its use within criminal investigations. This characterization is a critical input to future studies regarding the legal status of iFGG and its implications on the right to genetic privacy.
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