| 1. |
- Antonsson, Johan, et al.
(författare)
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Diffuse reflectance spectroscopy measurements for tissue type discrimination during deep brain stimulation
- 2008
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Ingår i: Journal of neural engineering. - : IOP Publishing. - 1741-2560 .- 1741-2552. ; 5:2, s. 185-190
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Tidskriftsartikel (refereegranskat)abstract
- Diffuse reflectance spectroscopy as a method for improving intracerebral guidance during functional neurosurgery has been investigated. An optical probe was developed for measurements during stereotactic and functional neurosurgery in man. The aim of the study was to investigate the spectral differences between white and grey matter and between white matter and functional targets. Diffuse reflectance spectroscopy measurements in ten patients were recorded at incremental steps towards and in three different functional targets (STN, GPi and Zi). The recorded spectra along the trajectory were sorted into white or grey matter, based on preoperative MRI images or the recorded spectral shape and intensity. The difference between tissue types was calculated as a quotient. Significant intensity differences between white and grey matter were found to be at least 14% (p < 0.05) and 20% (p < 0.0001) for MRI and spectral-sorted data respectively. The reflectance difference between white matter and the functional targets of GPi was higher than for STN and Zi. The results indicate that diffuse reflectance spectroscopy has a potential to be developed to a suitable complement to other intracerebral guidance methods.
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| 2. |
- Antonsson, Johan, et al.
(författare)
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In-vivo reflection spectroscopy measurements in pig brain during stereotactic surgery
- 2003
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Ingår i: Biophotonics West. - : SPIE. ; , s. 242-250
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Konferensbidrag (refereegranskat)abstract
- Radiofrequency (RF) lesioning in the human brain is a commonsurgical therapy for relieving severe pain as well as formovement disorders such as Parkinsonia. During the procedure a smallelectrode is introduced by stereotactic means towards a target arealocalized by CT or MRI. An RF-current is applied throughthe electrode tip when positioned in the target area. Thetissue in the proximity of the tip is heated bythe current and finally coagulated.The overall aim of this studywas to improve the RF-technique and its ability to estimatelesion size by means of optical methods. Therefore, the opticaldifferences between white and gray matter, as well as lesionedand unlesioned tissue were investigated. Reflection spectroscopy measurements in therange of 450-800 nm were conducted on fully anesthetized pigsduring stereotactic RF-lesioning (n=6). Light from a tungsten lamp wasguided to the electrode tip through optical fibers, inserted alonga 2 mm in diameter monopolar RF-electrode. Measurements were performedin steps of 0-10 mm from the target in eachhemisphere towards the entry point of the skull. In thecentral gray of the porcine brain measurements were performed bothbefore and after the creation of a lesion. A totalof 55 spectra were collected during this study. Correlation totissue type was done using post-operative MR-images. The spectral signaturefor white and gray matter differs significantly for the entirespectral range of 450-800 nm. Pre- and post-lesioning reflection spectroscopyshowed the largest differences below 600 and above 620 nm,which implies that lasers within this wavelength range may beuseful for in-vivo measurements of tissue optical changes during RF-lesioning.
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| 3. |
- Antonsson, Johan, 1973-
(författare)
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On optical methods for intracerebral measurements during stereotactic and functional neurosurgery : Experimental studies
- 2007
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Radio frequency (RF) lesioning and deep brain stimulation (DBS) are the two prevailing surgical treatments for movement disorders within the field of stereotactic and functional neurosurgery. For RF-lesioning, a small volume of brain tissue is coagulated and knowledge of the lesion size and growth is of great importance for the safety and outcome of the procedure. This thesis deals with adapting the laser Doppler perfusion monitoring (LDPM) technique for measurements in brain tissue during RF-lesioning. The relation between LDPM signal changes and developed lesion size was investigated. LDPM measurements were evaluated both in vitro (albumin protein solution) and in vivo in the porcine brain during RF-lesioning corresponding to a bilateral thalamotomy in man. The investigated signals from the LDPI measurements can be used for following the lesioning time course and to detect if a lesion was created, both in vitro and in the animal model. For the albumin model, both the total backscattered light intensity and the perfusion signal can be used as markers for estimating the final coagulation size, while in the animal model this conclusion was not statistical verified.Independent on surgical method, RF-lesioning or DBS, intracerebral guidance is an important aspect within stereotactic and functional neurosurgery. To increase the accuracy and precision of reaching the correct target, different methods for intracerebral guidance exist, such as microelectrode recording and impedance methods. In this thesis, the possibility of developing an optical intracerebral guidance method has been investigated. Diffuse reflectance spectroscopy served as technology and all measurements were performed stereotactically in both porcine and human brain. Measurements of white and gray matter showed large differences, with higher reflectivity for white brain matter, both in porcine and in human brain. For the human measurements during DBS-implants, large differences between white matter and functional targets were found. Additionally, differences between native and lesioned porcine brain matter were detected. Both studies support the idea of using diffuse reflectance spectroscopy for developing an intracerebral guidance method.
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| 4. |
- Antonsson, Johan, et al.
(författare)
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Optical measurements during experimental stereotactic radiofrequency lesioning
- 2006
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Ingår i: Stereotactic and Functional Neurosurgery. - : S. Karger. - 1011-6125 .- 1423-0372. ; 84:2-3, s. 118-124
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to evaluate in vivo a laser Doppler measurement system in porcine brain tissue during thermal lesioning. A 2-mm monopolar radiofrequency lesioning electrode was equipped with optical fibers in order to monitor the lesioning procedure. Laser Doppler and backscattered light intensity signals were measured along the electrode trajectory and during bilateral lesioning in the central gray (70, 80 and 90°C, n = 14). The time course of the coagulation process could be followed by optical recordings. Two separate groups of tissue were identified from the intensity signals. The changes in the perfusion levels in both groups displayed significant changes (p < 0.05, n = 48) at all temperature settings, while backscattered light intensity was significant for only one group at the different temperatures (p < 0.05, n = 39). These results indicate that optical measurements correlate with lesion development in vivo. The study also indicates that it is possible to follow the lesioning process intra-operatively.
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| 5. |
- Antonsson, Johan, et al.
(författare)
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Radio frequency electrode system for optical lesion size estimation in functional neurosurgery
- 2005
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Ingår i: Journal of Biomedical Optics. - : SPIE - International Society for Optical Engineering. - 1083-3668 .- 1560-2281. ; 10:3, s. 1-6
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Tidskriftsartikel (refereegranskat)abstract
- Radiofrequency(RF) lesioning in the human brain is one possible surgicaltherapy for severe pain as well as movement disorders. Oneobstacle for a safer lesioning procedure is the lack ofsize monitoring. The aim of this study was to investigateif changes in laser Doppler or intensity signals could beused as markers for size estimation during experimental RF lesioning.A 2 mm in diameter monopolar RF electrode was equippedwith optical fibers and connected to a digital laser Dopplersystem. The optical RF electrode's performance was equal to astandard RF electrode with the same dimensions. An albumin solutionwith scatterers was used to evaluate the intensity and laserDoppler signal changes during lesioning at 70, 80, and 90 °C.Significant signal changes were found for these three different clotsizes, represented by the temperatures (p<0.05, n=10). The volume, width, andlength of the created coagulations were correlated to the intensitysignal changes (r=0.88, n=30, p<0.0001) and to the perfusion signalchanges (r=0.81, n=30, p<0.0001). Both static and Doppler-shifted light canbe used to follow the lesioning procedure as well asbeing used for lesion size estimation during experimental RF lesioning.
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| 6. |
- Antonsson, Karin, 1963-
(författare)
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Holocene Climate in Central and Southern Sweden : Quantitative Reconstructions from Fossil Data
- 2006
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In quantitative palaeoecology modern species-environmental relationships can be statistically modelled, and recent development has made the calibration models more statistically robust. These models are used to transform fossil assemblages to quantitative estimates of past environmental conditions. The aim of this thesis is to infer Holocene temperatures from fossil pollen data sampled from lakes in central and southern Sweden. This reconstruction is done by using a north-European pollen-climate calibration model, which was extended with 37 modern pollen samples from the southern deciduous vegetation zone in Sweden within this project. A statistical method is used for deriving the pollen-climate calibration model, weighted averaging partial least square (WA-PLS) method. The long term trends in pollen inferred temperatures from this study reflect low, but rapidly rising temperatures in the early-Holocene, a trend that was temporarily interrupted by a cool period about 8500 cal yr BP, but continued after 8000 cal yr BP. A Holocene thermal maximum (HTM) with temperatures roughly 2°C higher than at present was recorded about 7000 cal yr BP and by 4000 cal yr BP pollen inferred temperatures starts to decline. In order to create a more comprehensive picture of past climate patterns in the investigated area inferred temperatures from this study are compared with independent palaeorecords, a stable oxygen isotope record for moisture variability (paper I) and chironomids for summer temperature (paper II). Taken all together, these records reflect a coherent Holocene climate pattern which also is supported by several studies from Scandinavia and the north Atlantic region. Pollen inferred temperatures and the moisture record are indicating markedly dry, continental climate conditions in southern Sweden during the HTM possibly as a result of reorganisations in regional atmosphere circulations. The local observations in this study of regional climate events, such as the cold period at about 8200 cal yr BP and the dry period at about 7000 to 4000 cal yr BP are of particular interest because they suggest that vegetation in the study region has responded sensitively both to long-term climatic trends and more transient climate events.
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| 7. |
- Antonsson, Karin, et al.
(författare)
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Holocene temperatures in Bohuslan, southwest Sweden : A quantitative reconstruction from fossil pollen data
- 2007
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Ingår i: Boreas. - : Wiley. - 0300-9483 .- 1502-3885. ; 36:4, s. 400-410
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Tidskriftsartikel (refereegranskat)abstract
- Inferred mean annual temperatures (Tann) from a radiocarbon-dated fossil pollen stratigraphy obtained from Lake Trehörningen, southwest Sweden, provide evidence of climate variability during the Holocene in the boreal-nemoral zone of Sweden. The record indicates low early Holocene temperatures, followed by a rising trend and maximum temperature values from about 7000 to 4000 cal. yr BP. During the later part of the Holocene until present day, a cooling trend is reflected in the temperature record. At about 8200 cal. yr BP, temperatures temporarily drop, and at 8000 cal. yr BP the rising temperature trend is recovered. Inferred mid-Holocene temperatures are about 2.5-3°C higher than at present, and also higher than other pollen-inferred mid-Holocene temperatures of 1.5-2.5°C further to the north and east in Scandinavia. The reconstructed long-term climate pattern in Trehörningen has an overall consistency with temperature reconstructions from Scandinavia, suggesting a regional climate link for the Holocene variability in Sweden. Holocene climate trends in Trehörningen also show a remarkably similar pattern with the classic work on postglacial climate change by Sernander (1893, Sernander 1909), Andersson (1909) and von Post (1924).
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| 8. |
- Antonsson, Karin, et al.
(författare)
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Quantitative palaeotemperature records inferred from fossil pollen and chironomid assemblages from Lake Gilltjärnen, northern central Sweden
- 2006
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Ingår i: Journal of Quaternary Science. - : Wiley. - 0267-8179 .- 1099-1417. ; 21:8, s. 831-841
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Tidskriftsartikel (refereegranskat)abstract
- Palaeotemperature reconstructions based on radiocarbon-dated fossil pollen and chironomid stratigraphies obtained from Lake Gilltjarnen provide evidence of climate changes during the last 11 000 years in the boreal zone of northern central Sweden. The records show consistent trends during the early and mid-Holocene, indicating low temperatures at 11 000-10 000 cal. yr BP, followed by a rising trend and a period of maximum values from about 7000 to 4000 cal. yr BP. At 3000 cal. yrBP the chironomid-inferred temperature values rise abruptly, deviating from the late-Holocene cooling trend indicated by the pollen-based reconstruction and most of the other palaeotemperature records from central Scandinavia, probably as a result of local limnological changes in Lake Gilltjarnen and its catchment. Comparison of the present results with a lake-level reconstruction from Lake Ljustjarnen, ca. 100 km southwest of Lake Gilitjarnen, shows that the low early-Holocene temperatures were associated with high lake-levels at 10500-8500 cal. yrBP, whereas low lake-levels and dry conditions prevailed during the period of high temperatures at between 7500 and 5000 cal. yr BP, probably due to high summer evapotranspiration and lower precipitation.
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| 9. |
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| 10. |
- Appelqvist, Hanna, et al.
(författare)
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Lysosome-Mediated Apoptosis is Associated with Cathepsin D-Specific Processing of Bid at Phe24,Trp48, and Phe183
- 2012
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Ingår i: Annals of Clinical and Laboratory Science. - : Institute for Clinical Science. - 0091-7370 .- 1550-8080. ; 42:3, s. 231-242
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Tidskriftsartikel (refereegranskat)abstract
- Bax-mediated permeabilization of the outer mitochondrial membrane and release of apoptogenic factors into the cytosol are key events that occur during apoptosis. Likewise, apoptosis is associated with permeabilization of the lysosomal membrane and release of lysosomal cathepsins into the cytosol. This report identifies proteolytically active cathepsin D as an important component of apoptotic signaling following lysosomal membrane permeabilization in fibroblasts. Lysosome-mediated cell death is associated with degradation of Bax sequestering 14-3-3 proteins, cleavage of the Box activator Bid, and translocation of Box to mitochondria, all of which were cathepsin D-dependent. Processing of Bid could be reproduced by enforced lysosomal membrane permeabilization, using the lysosomotropic detergent O-methyl-serine dodecylamine hydrochloride (MSDH). We identified three cathepsin D-specific cleavage sites in Bid, Phe24, Trp48, and Phe183. Cathepsin D-cleaved Bid induced Bax-mediated release of cytochrome c from purified mitochondria, indicating that the fragments generated are functionally active. Moreover, apoptosis was associated with cytosolic acidification, thereby providing a more favorable environment for the cathepsin D-mediated cleavage of Bid. Our study suggests that cytosolic cathepsin D triggers Bax-mediated cytochrome c release by proteolytic activation of Bid.
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