| 1. |
- Maldonado, Mario, et al.
(författare)
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Ketosis-prone diabetes: dissection of a heterogeneous syndrome using an immunogenetic and beta-cell functional classification, prospective analysis, and clinical outcomes
- 2003
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Ingår i: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 1945-7197 .- 0021-972X. ; 88:11, s. 5090-5098
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Tidskriftsartikel (refereegranskat)abstract
- Ketosis-prone diabetes is heterogeneous. Its causes could include novel beta-cell functional defects. To characterize such defects, 103 patients with diabetic ketoacidosis were evaluated for beta-cell autoimmunity and human leukocyte antigen (HLA) class II alleles, with longitudinal measurements of beta-cell function and biochemical and clinical parameters. They were classified into four A beta groups, based on the presence of glutamic acid decarboxylase (GAD)65, GAD67, or IA-2 autoantibodies (A+ or A-) and beta-cell functional reserve (beta+ or beta-). The group distribution was: 18 A+beta-, 23 A-beta-, 11 A+beta+, and 51 A-beta+. Collectively, the two beta- groups differed from the two beta+ groups in earlier onset and longer duration of diabetes, lower body mass index, less glycemic improvement, and persistent insulin requirement. HLA class II genotyping showed that the A-beta- group differed from the A+beta- group in having lower frequencies of two alleles strongly associated with
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| 2. |
- Popovski, Petar, et al.
(författare)
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EU FP7 INFSO-ICT-317669 METIS, D2.1, Requirement analysis and design approaches for 5G air interface
- 2013
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This document describes the problem space for the METIS research conducted in the radio link context. Firstly, a requirement analysis for the air interface design is conducted based on the test case descriptions presented in METIS deliverable D1.1. It follows an introduction of the research topics being pursued in the radio link research together with an illustration of how these topics are addressing the derived requirements. Moreover, it is shown which of thoserequirements address the needs of the METIS horizontal topics. To facilitate the achievement of these three objectives, a framework of General Requirements is introduced, which will be used throughout the project to assess and evaluate developed radio link solutions and to allow for measuring against the overall system performance goals.
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| 3. |
- Popovski, Petar, et al.
(författare)
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EU FP7 INFSO-ICT-317669 METIS, D2.2 Novel radio link concepts and state of the art analysis
- 2013
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This document provides a detailed overview of the Radio Link concepts being developed in METIS as well as a detailed analysis of the related state of the art. For each of the research topics identified for the radio link research covering flexible air interface, new waveforms,modulation and coding techniques as well as multiple access, medium access control and enablers for radio resource management, a detailed description of the aspects to be investigated will be given, going beyond the limits imposed by the systems operated today and their planned evolutions. The state of the art analysis, which is conducted for each of the research topics separately, covers current standards, their future evolutions as well as latest academic research. Elaborating on how the approaches followed in the radio link research may advance this state of the art carves a promising track towards innovative solutions addressing the challenges of future wireless communication.
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| 4. |
- Popovski, Petar, et al.
(författare)
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EU FP7 INFSO-ICT-317669 METIS, D2.3 Components of a new air interface - building blocks and performance
- 2014
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This document provides intermediate results of the concepts being developed in the radio link research of METIS. For each of the technology components (TeC) within the technology component clusters (TeCC), covering flexible air interface, new waveforms, modulation and coding techniques as well as multiple access, medium access control and enablers for radio resource management, key findings and conclusions collected so far are summarized in section 2. Continuative descriptions and research outcomes are given in the annex and referred publications.The results presented here will be extended in the further progress of the project, and they will be used in the next phase of the project to develop and refine the overall METIS system concept instantiated by the horizontal topics. The suitability of the single technology components for the overall system design being able to meet the wide range of requirements for 5G will then be evaluated.
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| 5. |
- Popovski, Petar, et al.
(författare)
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EU FP7 INFSO-ICT-317669 METIS, D2.4 Proposed solutions for new radio access
- 2015
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This deliverable represents the final report on the METIS radio link research. It provides a comprehensive and self-contained summary of all investigated technology components (TeCs), including evaluation results and conclusions on their potential for 5G. The METIS radio link research covers three main areas, which are considered key aspects for developing a self-contained air interface design for 5G: 1) Flexible air interface, 2) Waveforms, coding & modulation and transceiver design and 3) Multiple access, Medium Access Control (MAC) and Radio Resource Management (RRM). TeCs with similar research context and objectives have been grouped into clusters, whereof five have been selected as the most promising for 5G systems: From research area 1, TeC clusters providing key enablers for an air interface for ultra-dense networks (UDN) and for moving networks; from research area 2, multi-carrier transmission schemes with filtering; and from research area 3, novel access schemes for massive machine access as well as for non-orthogonal access.
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| 6. |
- Qayoom, Irfan, et al.
(författare)
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A biphasic nanohydroxyapatite/calcium sulphate carrier containing Rifampicin and Isoniazid for local delivery gives sustained and effective antibiotic release and prevents biofilm formation
- 2020
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
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Tidskriftsartikel (refereegranskat)abstract
- Long term multiple systemic antibiotics form the cornerstone in the treatment of bone and joint tuberculosis, often combined with local surgical eradication. Implanted carriers for local drug delivery have recently been introduced to overcome some of the limitations associated with conventional treatment strategies. In this study, we used a calcium sulphate hemihydrate (CSH)/nanohydroxyapatite (nHAP) based nanocement (NC) biomaterial as a void filler as well as a local delivery carrier of two standard of care tuberculosis drugs, Rifampicin (RFP) and Isoniazid (INH). We observed that the antibiotics showed different release patterns where INH showed a burst release of 67% and 100% release alone and in combination within one week, respectively whereas RFP showed sustained release of 42% and 49% release alone and in combination over a period of 12 weeks, respectively indicating different possible interactions of antibiotics with nHAP. The interactions were studied using computational methodology, which showed that the binding energy of nHAP with RFP was 148 kcal/mol and INH was 11 kcal/mol, thus varying substantially resulting in RFP being retained in the nHAP matrix. Our findings suggest that a biphasic ceramic based drug delivery system could be a promising treatment alternative to bone and joint TB.
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| 7. |
- Raina, Deepak Bushan, et al.
(författare)
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Biocomposite macroporous cryogels as potential carrier scaffolds for bone active agents augmenting bone regeneration
- 2016
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Ingår i: Journal of Controlled Release. - : Elsevier BV. - 0168-3659. ; 235, s. 365-378
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Tidskriftsartikel (refereegranskat)abstract
- Osteoinduction can be enhanced by combining scaffolds with bone morphogenic protein-2 (BMP-2). However, BMP's are known to also cause bone resorption. This can be controlled using bisphosphonates like zoledronic acid (ZA). In this study, we produced two different scaffolds containing silk-fibroin, chitosan, agarose and hydroxyapatite (HA) with and without bioactive glass. The aims of the study were to fabricate, physico-chemically characterize and evaluate the carrier properties of the scaffolds for recombinant human BMP-2 (rhBMP-2) and ZA. Scaffolds were characterized using various methods to confirm their composition. During cell-material interactions, both scaffolds exhibited gradual but sustained proliferation of both C2C12 and MSCs for a period of 6 weeks with augmentative effects on their phenotype indicated by elevated levels of alkaline phosphatase (ALP) cuing towards osteogenic differentiation. In-vitro effects of rhBMP-2 and ZA contained within both the scaffolds was assessed on MC3T3 preosteoblast cells and the results show a significant increase in the ALP activity of the cells seeded on scaffolds with rhBMP-2. Further, the scaffold with both HA and bioactive glass was considered for the animal study. In-vitro, this scaffold released nearly 25% rhBMP-2 in 21-days and the addition of ZA did not affect the release. In the animal study, the scaffolds were combined with rhBMP-2 and ZA, rhBMP-2 or implanted alone in an ectopic muscle pouch model. Significantly higher bone formation was observed in the scaffold loaded with both rhBMP-2 and ZA as seen from micro-computed tomography, histomorphometry and energy dispersive X-ray spectroscopy.
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| 8. |
- Sharma, Pushpa Kumari, et al.
(författare)
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Adsorptive behavior of Fe/Zn-modified nanobiochar for arsenic removal from naturally contaminated groundwater
- 2023
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Ingår i: Groundwater for Sustainable Development. - : Elsevier BV. - 2352-801X. ; 23, s. 101011-
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Tidskriftsartikel (refereegranskat)abstract
- Global groundwater is frequently discovered to have high arsenic (As) concentrations, critically endangered and potentially toxic to aquatic organisms and humans. This work investigates As(V) adsorption using raw nanobiochar (RnBC) and activated magnetic nanobiochar (AMnBC), which are synthesized via pre-treating rice straw biomass with ZnCl2 and FeCl3 and pyrolyzing at 500 °C, followed by ball milling. The highest adsorption capacity for AMnBC and RnBC was 130 μg/g and 38.67 μg/g, respectively, at alkaline water chemistry to mimic natural groundwater conditions. Different functional groups contributed by modifications are evident with As(V) adsorption using RnBC and AMnBC. Multilayer chemisorption may explain the adsorption of As(V) on biochar surfaces, as Freundulich isotherm and pseudo-second-order kinetic model are suggested. The synthesis cost for AMnBC and RnBC was $0.0147/g and $0.0099/g, respectively, which helped to determine the most effective and efficient method for As(V) adsorption. In this study, natural As-contaminated groundwater collected from Patna, Bihar (India), was treated for As(V) removal using AMnBC in natural environments. Thus, this study recommends that cost-effective modified biochar can effectively be used for As(V) elimination from naturally contaminated groundwater as well as surface water.
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| 9. |
- Teotia, Arun K., et al.
(författare)
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Exosome-Functionalized Ceramic Bone Substitute Promotes Critical-Sized Bone Defect Repair in Rats
- 2021
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Ingår i: ACS Applied Bio Materials. - : American Chemical Society (ACS). - 2576-6422. ; 4:4, s. 3716-3726
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Tidskriftsartikel (refereegranskat)abstract
- Ceramic biomaterials are promising alternatives to bone autografts. However, limited bioactivity affects their performance. Therefore, bioactive molecules and cells are often added to enhance their performance. Exosomes have emerged as cell-secreted vesicles, delivering proteins, lipids, and nucleic acids in a paracrine/endocrine fashion. We studied two complementary aspects required for exosome activity/therapy using purified exosomes: first, the intracellular uptake of labeled exosomes and second, the influence of delivered exosomes on cell behavior. Origin-specific differences in the characteristics of purified exosomes, quantification of time-dependent intracellular uptake of PKH-26-labeled exosomes by mesenchymal stem cells (MSCs) and preosteoblasts, and influence on cell behavior were evaluated. Furthermore, exosomes from osteoblasts and MSCs cultured under normal and osteogenic environments were isolated. There is little data available on the concentration and dose of exosomes required for bone regeneration. Therefore, equal amounts of quantified exosomes were implanted in vivo in rat tibia critical defects using a calcium sulfate-nano-hydroxyapatite nanocement (NC) bone filler as the carrier. Bone regeneration was quantified using micro-computed tomography and histology. Along with inducing early maturation and mineral deposition by primary preosteoblasts in vitro, exosome treatment also demonstrated a positive effect on bone mineralization in vivo. Our study concludes that providing a local delivery of exosomes loaded onto a slowly resorbing NC bone filler can provide a potential alternate to autografts as a bone substitute.
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| 10. |
- Teotia, Arun Kumar, et al.
(författare)
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Gelatin-Modified Bone Substitute with Bioactive Molecules Enhance Cellular Interactions and Bone Regeneration
- 2016
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 8:17, s. 10775-10787
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Tidskriftsartikel (refereegranskat)abstract
- In this work, we have synthesized injectable bone cement incorporated with gelatin to enhance cellular interaction. Human osteosarcoma Saos-2 cells derived bone morphogenetic proteins (BMP's) and a bisphosphonate (zoledronic acid (0.2 mM)) were also incorporated to cement. In vitro studies conducted using Saos-2 demonstrated enhanced cell proliferation on gelatin (0.2%w/v) cement. The differentiation of C2C12 mouse myoblast cells into bone forming cells showed 6-fold increase in ALP levels on gelatin cement. Polymerase chain reaction (PCR) for bone biomarkers showed osteoinductive potential of gelatin cement. We investigated efficacy for local delivery of these bioactive molecules in enhancing bone substitution qualities of bone cements by implanting in 3.5 mm critical size defect in tibial metaphysis of wistar rats. The rats were sacrificed after 12 weeks and 16 weeks post implantation. X-ray, micro-CT, histology, and histomorphometry analysis were performed to check bone healing. The cement materials slowly resorbed from the defect site leaving HAP creating porous matrix providing surface for bone formation. The materials showed high biocompatibility and initial bridging was observed in all the animals but maximum bone formation was observed in animals implanted with cement incorporated with zoledronic acid followed by cement with BMP's compared to other groups.
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