| 1. |
- Asplund, Stig-Börje, 1973-
(författare)
-
Läsning som identitetsskapande handling. : Gemenskapande och utbrytningsförsök i fordonspojkars litteratursamtal.
- 2010
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In this dissertation literature discussions which include students of Vehicle Engineering at a Swedish upper secondary school will be discussed. The students are all boys. The study is focused on these boys’ small-group literature discussions, and the aim of the study is to describe what happens when they discuss four of the novels that form an integral part in the context of their Swedish studies. Principal fields of interest are the readings and the reception that the boys give utterance to in the literature discussions, as well as the identity-constructing processes which set in when they interact in the light of what they have read.The empirical material consists of eleven video recorded small-group discussions, and a conversational analytic perspective is combined with a reception theory perspective in the dissertation.The study shows that a sense of community is vital to the core of the boys’ literature discussions, and this has an influence on the reception and the readings that come up in the discussions. The boys are anxious to come to an agreement and voices which threaten to shatter the sense of community that has been constructed in the discussions are opposed in different ways. The literature discussions are not only arenas where the boys construct themselves as readers but are also considered as a forum where various identity-constructing practices take place.As readers these boys are bound to what can be read, and what cannot be read, in literary texts, but it is possible to see a certain development in their readings towards a more complex attitude, such as they find expression in the discussions. The boys also carry out the literature discussions, and the constructed sense of community is based on the reading of the novel to be discussed in a small-group discussion. Thus the boys take on the responsibility for their own learning as well.Many of the processes in which the boys are involved during the literature discussions could be described as processes of social reproduction, but various dividing lines which could be identified in the study also show that there are openings for other directions of the discussions.
|
|
| 2. |
- Alam, Assad, et al.
(författare)
-
Cooperative driving according to Scoop
- 2011
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- KTH Royal Institute of Technology and Scania are entering the GCDC 2011 under the name Scoop –Stockholm Cooperative Driving. This paper is an introduction to their team and to the technical approach theyare using in their prototype system for GCDC 2011.
|
|
| 3. |
|
|
| 4. |
- Amisten, Stefan, et al.
(författare)
-
Activation of imidazoline receptor I-2, and improved pancreatic beta-cell function in human islets
- 2018
-
Ingår i: Journal of Diabetes and Its Complications. - : Elsevier BV. - 1056-8727. ; 32:9, s. 813-818
-
Tidskriftsartikel (refereegranskat)abstract
- Aim: The impact of BL11282, an imidazoline receptor (NISCH) agonist, on potentiation of glucose-stimulated insulin secretion (GSIS) from isolated human non-diabetic (ND) and type 2 diabetic (T2D) islets was investigated. Methods: Analysis of mRNA was performed by RNA-sequencing and qPCR Insulin and cAMP by RIA and EUSA respectively. Results: RNA-sequencing data revealed that NISCH is highly expressed in fat tissues, islets, liver and muscles, with eight detectable splice variants of transcripts in islets. NISCH had a positive correlation with GLP-1 (GLP1R) and GIP (GIPR) receptor transcripts. The expression of NISCH was confirmed by qPCR in human islets. NISCH and GLP1R were comparably higher expressed in mouse islets compared to human islets. GSIS was dose-dependently potentiated by BL11282 from incubated islets of ND and T2D human islet donors. The insulinotropic action of BL11282 was associated with increased cAMP. While the harmful effect of high glucose on reductive capacity of islet cells was enhanced by glibenclamide during long-term culture, it was counteracted by BL11282 or Bt2-cAMP. BL11282 also increased proliferation of INS-1 cells during long-time culture. Conclusion: Our data suggest that BL11282 potentiates GSIS by an action involving cAMP/PKA system and BL11282 could be an attractive insulinotropic and beta-cell protective agent. (C) 2018 Elsevier Inc. All rights reserved.
|
|
| 5. |
- Andersson, J. Y., et al.
(författare)
-
Quantum structure based infrared detector research and development within Acreo's centre of excellence IMAGIC
- 2010
-
Ingår i: Infrared physics & technology. - : Elsevier BV. - 1350-4495 .- 1879-0275. ; 53:4, s. 227-230
-
Tidskriftsartikel (refereegranskat)abstract
- Acreo has a long tradition of working with quantum structure based infrared (IR) detectors and arrays. This includes QWIP (quantum well infrared photodetector), QDIP (quantum dot infrared photodetector), and InAs/GaInSb based photon detectors of different structure and composition. It also covers R&D on uncooled microbolometers. The integrated thermistor material of such detectors is advantageously based on quantum structures that are optimised for high temperature coefficient and low noise. Especially the SiGe material system is preferred due to the compatibility with silicon technology. The R&D work on IR detectors is a prominent part of Acreo's centre of excellence "IMAGIC" on imaging detectors and systems for non-visible wavelengths. IMAGIC is a collaboration between Acreo, several industry partners and universities like the Royal Institute of Technology (KTH) and Linkoping University. (C) 2010 Elsevier B.V. All rights reserved.
|
|
| 6. |
|
|
| 7. |
- Asplund, Matthias, et al.
(författare)
-
Condition monitoring of rolling stock wheels : approach towards maintenance decision making
- 2014
-
Ingår i: 27th International Congress on Condition Monitoring and Diagnostic Engineering (COMADEM 2014). - : COMADEM International.
-
Konferensbidrag (refereegranskat)abstract
- Due to the more or less fixed inherent capacity of a railway system, capacity consuming events like failures within a railway network should be kept to a minimum. This could be achieved by the use of existing and new condition monitoring systems which can detect, report and predict failure events in an early stage. Demands for higher service quality, higher capacity, network availability and track quality together with less human intervention on tracks, drive the development of railway condition monitoring systems.Failure driven capacity consumption due to worn or defected rolling stock wheels have a big impact on the capacity and the infrastructure condition. Wheel defects such as out-of-round wheels, generates high forces, and could result in large capacity consumption especially for areas with cold climate conditions. Bad wheels cause even higher track wear that reduce the life length of the track. Wheels with fatigue defects could also influence the track safety issues. This paper presents how different wheel defects can be monitored; together with a review of the most common wayside condition monitoring systems on the Swedish railway network. The study also describes how the decision making process could take advantage of the condition monitoring data in order to increase the achieved network capacity.
|
|
| 8. |
- Asplund, Olof
(författare)
-
Data integration and visualization in type 2 diabetes research and care: From biological mechanisms to precision medicine.
- 2021
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Background. Type 2 diabetes (T2D) is a complex heterogeneous disorder affecting multiple organs. Gene expression studies in organs central to T2D, such as pancreatic islets and skeletal muscle will help elucidate and disentangle molecular mechanisms underlying T2D. Deep phenotyping using commonly measured clinical variables allows for the identification of clinically relevant T2D subgroups, representing a step towards individualized medicine. Creation and use of interactive, web-based tools can facilitate the application of these findings by researchers and clinicians.Aim. To analyze and investigate diabetes-related aberrations in gene expression in skeletal muscle and islets using bioinformatics approaches. To expand methodologies for diabetes sub-classification using anthropometric and biomarker data from T2D patients for clinical application. Toc reate and refine novel web applications providing unique resources for investigation of the metabolic abnormalities occurring in diabetes. Methods. In paper I, curated, public skeletal muscle microarray gene expression data were used to analyze alterations in gene expression in relation to diabetes-related phenotypes such as BMI, T2D and age. In Paper II, RNA sequencing data on human islets from 188 donors were used to analyze the association between gene expression and diabetes status, BMI, and HbA1c, among other diabetes-related phenotypes. In paper III, a combined analysis of human islets from 420 donors were used to investigate the relationship between genetic variation and gene expression. In paper IV, a total of 43 samples from islet, liver, kidney and skin from human embryos/fetuses between 7-14 weeks post conception were sequenced using RNA sequencing. Gene expression of fetal tissues in relation to corresponding tissues in adults from GTEx were used to identify genes important in development. In paper V, clinical data including age of onset, sex, BMI, HbA1c, HOMA2IR, HOMA2B, and GAD antibodies collected at diagnosis from 8,406 T2D patients were used to implement a novel model of classification of T2D based on previously-published findings. Results. In Paper I, phenotype data and gene expression data, as well as differential expression analyses of age, gender, diabetes, and acute training was used to create MuscleAtlasExplorer, an interactive data visualization web application providing complex functions for analysis of gene expression and phenotype data in skeletal muscle. In Paper II, a web application for gene look-ups, IsletGeneView, was created, providing gene-centric information for researchers with gene expression in human islets in relation to diabetes, HbA1c, BMI and co-expression with secretory genes, elucidating the complex phenotype-gene expression relationship in diabetic islets. In paper III, novel eQTLs in human islets were identified, increasing the insight into genetic variants influencing gene expression in human islets. In paper IV, FLEET was created, a web application atlas of gene expression in human fetal and adult islets, kidney, liver and skin. In paper V, a novel method for diabetes sub-classification with confidence scoring was devised and implemented. A novel software system for sub-classification of diabetes was created incorporating the sub-classification method, allowing for automatic classification of patients and entire diabetes research cohorts. Conclusion. Taken together, this thesis shows the possibilities of using data visualization and web development to create resources for elucidating biological mechanisms underlying T2D. The resulting data is readily applicable and can be used to support functional studies. Furthermore, an automated system for diabetes sub-classification is presented and can facilitate support to clinicians, representing a first step towards individualized therapy.
|
|
| 9. |
- Asplund, Olof, et al.
(författare)
-
Islet Gene View-a tool to facilitate islet research
- 2022
-
Ingår i: Life Science Alliance. - : Life Science Alliance, LLC. - 2575-1077. ; 5:12
-
Tidskriftsartikel (refereegranskat)abstract
- Characterization of gene expression in pancreatic islets and its alteration in type 2 diabetes (T2D) are vital in understanding islet function and T2D pathogenesis. We leveraged RNA sequencing and genome-wide genotyping in islets from 188 donors to create the Islet Gene View (IGW) platform to make this information easily accessible to the scientific community. Expression data were related to islet phenotypes, diabetes status, other islet-expressed genes, islet hormone-encoding genes and for expression in insulin target tissues. The IGW web application produces output graphs for a particular gene of interest. In IGW, 284 differentially expressed genes (DEGs) were identified in T2D donor islets compared with controls. Forty percent of DEGs showed cell-type enrichment and a large proportion significantly co-expressed with islet hormone-encoding genes; glucagon (GCG, 56%), amylin (IAPP, 52%), insulin (INS, 44%), and somatostatin (SST, 24%). Inhibition of two DEGs, UNC5D and SERPINE2, impaired glucose-stimulated insulin secretion and impacted cell survival in a human beta-cell model. The exploratory use of IGW could help designing more comprehensive functional follow-up studies and serve to identify therapeutic targets in T2D.
|
|
| 10. |
- Asplund, Olof, et al.
(författare)
-
MuscleAtlasExplorer : a web service for studying gene expression in human skeletal muscle
- 2020
-
Ingår i: Database: the journal of biological databases and curation. - : Oxford University Press (OUP). - 1758-0463. ; 2020
-
Tidskriftsartikel (refereegranskat)abstract
- MuscleAtlasExplorer is a freely available web application that allows for the exploration of gene expression data from human skeletal muscle. It draws from an extensive publicly available dataset of 1654 skeletal muscle expression microarray samples. Detailed, manually curated, patient phenotype data, with information such as age, sex, BMI and disease status, are combined with skeletal muscle gene expression to provide insights into gene function in skeletal muscle. It aims to facilitate easy exploration of the data using powerful data visualization functions, while allowing for sample selection, in-depth inspection and further analysis using external tools. Availability: MuscleAtlasExplorer is available at https://mae.crc.med.lu.se/mae2 (username 'muscle' and password 'explorer' pre-publication).
|
|
