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| 2. |
- Ulfvin, A, et al.
(författare)
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Expression of glycolipid blood group antigens in single human kidneys: change in antigen expression of rejected ABO incompatible kidney grafts.
- 1993
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Ingår i: Kidney international. - 0085-2538. ; 44:6, s. 1289-97
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Tidskriftsartikel (refereegranskat)abstract
- Total neutral glycolipid fractions were separated into molecular species on thin-layer chromatography plates and detected by immunostaining with monoclonal anti-blood group antibodies. Blood group A antigens based on type 1, 2, 3 and 4 carbohydrate core saccharides were present in kidneys of A1 and A1B individuals. Blood group A2 individuals expressed only small amounts of A antigen compared to A1 individuals especially of the type 3 and 4 compounds. Kidneys from non-secretor individuals contained less A antigen compared to secretor individuals, and in both groups a variation in the antigen expression between single individuals was noted. Blood group A type 2 and 3 (which is an extension of A type 2) antigens were present both as basic 6 and 9 sugar structures as well as extended saccharide chains migrating in the 8 to 11 sugar interval. In contrast, the type 1 chain based A and Lewis antigens were only present as their basic 5 to 7 sugar chains, and no elongated structures were found. Four cases of A2 kidneys initially transplanted into O recipients and removed after 5, 12, 21 days and 4 years, respectively, were also analyzed. Two of these kidneys, originating from the same donor, showed a difference in A antigen expression. The kidney functioning for four years (lost due to chronic rejection) completely lacked X antigen with five sugar residues (present in all other individuals) and contained a large amount of A antigens.(ABSTRACT TRUNCATED AT 250 WORDS)
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| 4. |
- Hegedüs, Z., et al.
(författare)
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Imaging modalities at the Swedish Materials Science beamline at PETRA III
- 2019
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Ingår i: IOP Conference Series. - : Institute of Physics Publishing.
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Konferensbidrag (refereegranskat)abstract
- High-energy synchrotron radiation has been demonstrated to be a powerful tool for materials characterization. The development of novel methodologies is still ongoing, driven by major technological advances regarding the available source brilliance and efficient large area detectors. The Swedish Materials Science beamline at PETRA III is dedicated to materials characterization by high-energy X-rays and scheduled to enter into user operation starting August 2019. The beamline has been designed in particular for the combination of two complementary techniques: wide and small angle scattering and imaging. The beamline design is presented briefly and the different techniques are reviewed with regard to the contrast mechanisms and the ability to obtain spatially resolved information.
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| 6. |
- Rubens, Ulysse, et al.
(författare)
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BIAFLOWS : A Collaborative Framework to Reproducibly Deploy and Benchmark Bioimage Analysis Workflows.
- 2020
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Ingår i: Patterns (New York, N.Y.). - : Elsevier BV. - 2666-3899. ; 1:3
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Tidskriftsartikel (refereegranskat)abstract
- Image analysis is key to extracting quantitative information from scientific microscopy images, but the methods involved are now often so refined that they can no longer be unambiguously described by written protocols. We introduce BIAFLOWS, an open-source web tool enabling to reproducibly deploy and benchmark bioimage analysis workflows coming from any software ecosystem. A curated instance of BIAFLOWS populated with 34 image analysis workflows and 15 microscopy image datasets recapitulating common bioimage analysis problems is available online. The workflows can be launched and assessed remotely by comparing their performance visually and according to standard benchmark metrics. We illustrated these features by comparing seven nuclei segmentation workflows, including deep-learning methods. BIAFLOWS enables to benchmark and share bioimage analysis workflows, hence safeguarding research results and promoting high-quality standards in image analysis. The platform is thoroughly documented and ready to gather annotated microscopy datasets and workflows contributed by the bioimaging community.
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| 7. |
- Wallgren, Annacarin, 1964, et al.
(författare)
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Direct allorecognition promotes activation of bystander dendritic cells and licenses them for Th1 priming: a functional link between direct and indirect allosensitization.
- 2005
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Ingår i: Scandinavian journal of immunology. - : Wiley. - 0300-9475 .- 1365-3083. ; 62:3, s. 234-42
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Tidskriftsartikel (refereegranskat)abstract
- T-cell sensitization to indirectly presented alloantigens (indirect pathway of allorecognition) plays a critical role in chronic rejection. The usual very efficient priming of such self-restricted, T helper type 1 (Th1)-deviated CD4+ T cells obviously conflicts with the fact that allogeneic MHC molecules are poorly immunogenic per se. The aim of the present study is to elucidate whether direct allosensitization induces production of inflammatory mediators that may affect recruitment and activation of immature bystander (host) dendritic cells (DC). These potential mechanisms were studied in vitro by conducting primary allogeneic mixed leucocyte reactions (MLR), mimicking the priming phase in secondary lymphoid organs, and secondary MLR, mimicking the effector phase within the graft. Primary, and particularly secondary, MLR supernatants were found to contain high levels of monocyte/immature DC-recruiting CC chemokines and pro-inflammatory cytokines. Exposure of immature DC to primary or secondary MLR supernatants was found to upregulate CD40 expression and further enhanced lipopolysaccharide-induced interleukin-12 (IL-12) p70 production. Secondary MLR supernatants additionally induced upregulation of CD86 and deviated allogeneic T-cell responses towards Th1 (enhanced interferon-gamma production without concomitant induction of detectable IL-4 or IL-10 production). These findings indicate that direct allorecognition may act as a Th1-deviating adjuvant for indirect allosensitization.
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