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- Annerstedt, Claes, 1953, et al.
(författare)
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Idrott Hälsa Livsstil
- 2000
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Bok (övrigt vetenskapligt/konstnärligt)
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- Eskeland, Sveinung, et al.
(författare)
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Temporal patterns of gene expression in response to inoculation with a virulent Anaplasma phagocytophilum strain in sheep
- 2023
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Ingår i: Scientific Reports. - : Springer. - 2045-2322. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to characterize the gene expression of host immune- and cellular responses to a Norwegian virulent strain of Anaplasma phagocytophilum, the cause of tick-borne fever in sheep. Ten sheep were intravenously inoculated with a live virulent strain of A. phagocytophilum. Clinical-, observational-, hematological data as well as bacterial load, flow cytometric cell count data from peripheral blood mononuclear cells and host's gene expression post infection was analysed. The transcriptomic data were assessed for pre-set time points over the course of 22 days following the inoculation. Briefly, all inoculated sheep responded with clinical signs of infection 3 days post inoculation and onwards with maximum bacterial load observed on day 6, consistent with tick-borne fever. On days, 3-8, the innate immune responses and effector processes such as IFN1 signaling pathways and cytokine mediated signaling pathways were observed. Several pathways associated with the adaptive immune responses, namely T-cell activation, humoral immune responses, B-cell activation, and T- and B-cell differentiation dominated on the days of 8, 10 and 14. Flow-cytometric analysis of the PBMCs showed a reduction in CD4+CD25+ cells on day 10 and 14 post-inoculation and a skewed CD4:CD8 ratio indicating a reduced activation and proliferation of CD4-T-cells. The genes of important co-stimulatory molecules such as CD28 and CD40LG, important in T- and B-cell activation and proliferation, did not significantly change or experienced downregulation throughout the study. The absence of upregulation of several co-stimulatory molecules might be one possible explanation for the low activation and proliferation of CD4-T-cells during A. phagocytophilum infection, indicating a suboptimal CD4-T-cell response. The upregulation of T-BET, EOMES and IFN-γ on days 8-14 post inoculation, indicates a favoured CD4 Th1- and CD8-response. The dynamics and interaction between CD4+CD25+ and co-stimulatory molecules such as CD28, CD80, CD40 and CD40LG during infection with A. phagocytophilum in sheep needs further investigation in the future.
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- Hilde, Gunvor, et al.
(författare)
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Continence and pelvic floor status in nulliparous women at midterm pregnancy.
- 2012
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Ingår i: International urogynecology journal. - : Springer Science and Business Media LLC. - 1433-3023 .- 0937-3462.
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Tidskriftsartikel (refereegranskat)abstract
- INTRODUCTION AND HYPOTHESIS: A Cochrane review recommends antenatal pelvic floor muscle training (PFMT) in urinary incontinence (UI) prevention. The aim of the study was to investigate nulliparous pregnant women's knowledge about and practising of PFMT, their pelvic floor muscle (PFM) function, and ability to contract correctly. It was hypothesized that continent women had higher PFM strength and endurance than women with UI. METHODS: Three hundred nulliparous women at gestational week 18-22 were included in a cross-sectional study. Vaginal resting pressure, maximum voluntary contraction, and PFM endurance were measured by manometer. UI was assessed by International Consultation on Incontinence Questionnaire Urinary Incontinence Short Form (ICIQ-UI-SF). Comparisons of PFM function in continent women and women with UI were analyzed using independent-samples t test. Mean differences with 95% confidence interval (CI) are presented. RESULTS: Of 300 women, 89% had heard of PFMT at mid pregnancy, and 35% performed PFMT once or more a week. After thorough instruction 4% were unable to contract correctly. Thirty-five percent reported UI, of whom 48% performed PFMT once or more a week. Continent women had significantly higher PFM strength and endurance when compared with women having UI, with mean differences of 6.6 cmH(2)O (CI 2.3-10.8, p=0.003), and 41.5 cmH(2)Osec (CI 9.8-73.1, p=0.010), respectively. No difference was found for vaginal resting pressure (p=0.054). CONCLUSIONS: Most nulliparous pregnant women knew about PFMT. Thirty-five percent performed PFMT once or more a week. Incontinent nulliparous pregnant women had weaker PFM than their continent counterparts. More emphasis on information regarding PFM function and PFMT is warranted during pregnancy.
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- Lindgren, Anna, 1981-
(författare)
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Incontinence, physical activity, and pelvic floor muscle training in female pelvic cancer survivors after radiotherapy
- 2020
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Background: Cancer treatment continues to improve, contributing to an ever-growing population of cancer survivors. Pelvic cancer survivors (PCS) constitute the second largest group of female cancer survivors after breast cancer. Many female PCS have been treated with radiotherapy as a part of their cancer treatment. Unfortunately, like all effective cancer treatments, pelvic radiotherapy is associated with a risk of subsequent, unwanted side effects. Some side effects remain or persist long after the end of treatment and some are even lifelong. A common and burdensome side effect after pelvic radiotherapy is urinary and/or fecal incontinence. Incontinence is known to negatively affect quality of life (QoL) and physical activity levels. Physical activity contributes to several positive health effects. In cancer survivors, it may reduce the risk of recurrence and even the mortality risk. Cancer survivors in general, and female PCS in particular, tend to be less physically active after cancer treatment than before treatment. When suffering from urinary and even fecal incontinence, pelvic floor muscle training (PFMT) is recommended as a first-line treatment for the general population. In addition to decreased incontinence levels, PFMT may contribute to increased physical activity and better QoL. However, little attention is given to PFMT as a potential treatment for incontinence in the Swedish national care program for pelvic cancer rehabilitation. Furthermore, there is as yet no evidence that PFMT is as effective in female PCS as in female non-cancer survivors. The effectiveness of PFMT cannot be taken for granted because female PCS survivors often have treatmentinduced damage to structures in the pelvic floor that might affect its applicability. However, the problem of incontinence among female PCS remains, along with the fact that they tend to be less physically active than other cancer survivors. Indeed, this is an important research area and a necessary problem for health-care providers to resolve, not least for physiotherapists.Aim: The overall aim of this thesis is to improve the understanding of female PCS’ experiences of incontinence in relation to physical activity, QoL, and rehabilitative efforts, including PFMT. This includes gaining increased knowledge about the relation between incontinence and physical activity in the form of exercise and QoL, and whether PCS experience that physiotherapy contributes in a valuable way to reducing their incontinence. This could enable the development of meaningful physiotherapeutic interventions, that PCS can and are willing to engage in, to achieve a potential reduction in incontinence, as well as increased QoL and activity levels.Methods: The thesis includes four different studies, using three different methods, all conducted with female PCS. Studies I (n=13) and IV (n=11) are qualitative individual interview studies, using semi-structured interview guides. Study II is a cohort-based cross-sectional observational study (n=578) and Study III is a prospective cohort-based observational study (n=260).Results: Female PCS reported an absence of information regarding incontinence as a potential side effect of radiotherapy treatment. They experienced that incontinence prevented them from being as physically active as before treatment, and that incontinence of urine and feces impaired several aspects of QoL, including sexual health. They lacked potential rehabilitative options beyond conventional pelvic cancer rehabilitation. After practicing PFMT for three months, they found it a valuable rehabilitative measure for incontinence. They also experienced the physiotherapeutic support and guidance as valuable in teaching them how to contract the pelvic floor muscles correctly and providing individual guidance regarding dose, frequency, and progression of the training. In Study II, 67% of female PCS exercised at least once a week, while 33% exercised less than once a week. Women who reported leakage of large or all volume of feces (multivariable analysis) were statistically significantly more likely to exercise less than once a week. A similar co-variation was seen among women who reported leakage of moderate to large volumes of urine (univariate analysis). This, however, was not statistically significant in a multivariable analysis. When exercising on a weekly basis, they reported less frequently depressed mood and better QoL, compared to those who exercised less than once a week. Three months after an individually designed intervention program, in line with the conventional pelvic cancer rehabilitation offered within Swedish healthcare today, female PCS reported statistically significantly lower levels of urinary and fecal incontinence. However, no statistically significant changes in frequency of exercise were seen.Conclusion: Incontinence was a barrier to physical activity and exercise, and it reduced QoL and impaired sexual health in female PCS. When experiencing incontinence, and in particular fecal incontinence, female PCS were less likely to exercise on a weekly basis. Female PCS who exercise at least once a week experienced better QoL and less frequently depressed mood than PCS who were not exercising every week. Female PCS did not exercise more often after conventional pelvic cancer rehabilitation, not even after incontinence levels were reduced. Female PCS had a positive attitude towards PFMT. After at least three months’ experience of practicing PFMT, they found it a valuable rehabilitative effort for incontinence. They also found physiotherapeutic support and guidance to be of great importance. Female PCS expressed a need for better information routines regarding side effects, such as incontinence, after cancer treatment. They also expressed a need for better information routines, including accessibility of additional rehabilitative efforts, beyond the conventional pelvic cancer rehabilitation offered today, when suffering from incontinence of urine and/or feces.
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