| 1. |
- Hollandi, R., et al.
(författare)
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nucleAIzer : A Parameter-free Deep Learning Framework for Nucleus Segmentation Using Image Style Transfer
- 2020
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Ingår i: Cell Systems. - : Elsevier BV. - 2405-4712. ; 10:5, s. 453-458.e6
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Tidskriftsartikel (refereegranskat)abstract
- Single-cell segmentation is typically a crucial task of image-based cellular analysis. We present nucleAIzer, a deep-learning approach aiming toward a truly general method for localizing 2D cell nuclei across a diverse range of assays and light microscopy modalities. We outperform the 739 methods submitted to the 2018 Data Science Bowl on images representing a variety of realistic conditions, some of which were not represented in the training data. The key to our approach is that during training nucleAIzer automatically adapts its nucleus-style model to unseen and unlabeled data using image style transfer to automatically generate augmented training samples. This allows the model to recognize nuclei in new and different experiments efficiently without requiring expert annotations, making deep learning for nucleus segmentation fairly simple and labor free for most biological light microscopy experiments. It can also be used online, integrated into CellProfiler and freely downloaded at www.nucleaizer.org. A record of this paper's transparent peer review process is included in the Supplemental Information. Microscopy image analysis of single cells can be challenging but also eased and improved. We developed a deep learning method to segment cell nuclei. Our strategy is adapting to unexpected circumstances automatically by synthesizing artificial microscopy images in such a domain as training samples.
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| 2. |
- Grigoryan, L, et al.
(författare)
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Self-medication with antimicrobial drugs in Europe
- 2006
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Ingår i: Emerging infectious diseases. - : Centers for Disease Control and Prevention (CDC). - 1080-6040 .- 1080-6059. ; 12:3, s. 452-459
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Tidskriftsartikel (refereegranskat)
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| 3. |
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| 4. |
- Nehme, Ralda, et al.
(författare)
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The 22q11.2 region regulates presynaptic gene-products linked to schizophrenia
- 2022
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Ingår i: Nature Communications. - : Nature Publishing Group. - 2041-1723. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- It is unclear how the 22q11.2 deletion predisposes to psychiatric disease. To study this, we generated induced pluripotent stem cells from deletion carriers and controls and utilized CRISPR/Cas9 to introduce the heterozygous deletion into a control cell line. Here, we show that upon differentiation into neural progenitor cells, the deletion acted in trans to alter the abundance of transcripts associated with risk for neurodevelopmental disorders including autism. In excitatory neurons, altered transcripts encoded presynaptic factors and were associated with genetic risk for schizophrenia, including common and rare variants. To understand how the deletion contributed to these changes, we defined the minimal protein-protein interaction network that best explains gene expression alterations. We found that many genes in 22q11.2 interact in presynaptic, proteasome, and JUN/FOS transcriptional pathways. Our findings suggest that the 22q11.2 deletion impacts genes that may converge with psychiatric risk loci to influence disease manifestation in each deletion carrier.
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| 5. |
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| 6. |
- Clayton, Govinda, et al.
(författare)
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Introducing the ETH/PRIO Civil Conflict Ceasefire Dataset
- 2023
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Ingår i: Journal of Conflict Resolution. - : Sage Publications. - 0022-0027 .- 1552-8766. ; 67:7-8, s. 1430-1451
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Tidskriftsartikel (refereegranskat)abstract
- This article introduces the Civil Conflict CeaseFire (CF) dataset. The CF data covers all ceasefires in civil conflict between 1989 and 2020, including multilateral, bilateral and unilateral arrangements, ranging from verbal arrangements to detailed written agreements. In total, the CF data includes 2202 ceasefires across 66 countries and 109 civil conflicts. The data feature information on the actors involved in the ceasefire, and the class, purpose, coverage, and end date of the ceasefire. The CF data provide an empirical basis to assess the conditions that give rise to ceasefires, how ceasefires affect the dynamics of conflict, and the role of a ceasefire in the peace process. This article presents the rationale underlying the data collection, the coding rules and procedures, and how this data can be used for analysis.
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| 7. |
- Desjardins, L, et al.
(författare)
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Correlation of plasma coagulation parameters with thromboprophylaxis, patient characteristics, and outcome in the MEDENOX study
- 2004
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Ingår i: Archives of pathology & laboratory medicine. - 0003-9985. ; 128:5, s. 519-526
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Tidskriftsartikel (refereegranskat)abstract
- 0 Context.-Plasma anti-Xa and anti-IIa activities correlate with the dose of low-molecular-weight heparin, and D-dimer and thrombin-antithrombin complexes are markers of procoagulant activity. Objective.-To investigate the relationship between plasma coagulation parameters and patient characteristics, including renal function, thromboprophylaxis, and incidence of venous thromboembolism (VTE) in the MEDENOX study population. Design.-Controlled, multicenter, double-blind, randomized study. Patients.-Two hundred twenty-four acutely ill medical patients. Interventions.-Either 20 or 40 mg of enoxaparin administered subcutaneously or a placebo once daily for 10 (+/-4) days. ain Outcome Measures.-VTE and plasma anti-Xa and anti-IIa activities, D-dimer, and thrombin-antithrombin levels in blood collected before prophylaxis was given (day 0) and after the last injection of the study drug. Results and Conclusions.-Anti-Xa activity correlated with the dose of enoxaparin. In patients with mild or moderate renal impairment, there was no significant relationship between anti-Xa activity and the creatinine clearance rate. D-dimer concentrations were lower at day 10 (+/-4) in the 40-mg group, which had a 63% lower VTE incidence, than at day 0. No venographically confirmed thromboses were found in patients with a normal D-dimer I concentration (<0.5 mug/mL [0.5 mg/L]). D-dimer levels were higher in patients with VTE than in those without VTE, but no predictive value could be demonstrated for individual patients.
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| 8. |
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| 9. |
- Trujillo, M J, et al.
(författare)
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Dietary recommendations for patients allergic to Anisakis simplex
- 2002
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Ingår i: Allergologia et Immunopathologia. - 0301-0546. ; 30:6, s. 311-314
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Anisakis simplex, a fish parasite, causes allergic urticaria, angioedema and anaphylactic shock through an IgE-mediated hypersensitivity mechanism. Consensus on the dietary recommendations that should be given to allergic patients is lacking. Our objective was to evaluate the usefulness of different types of diets in preventing further reactions in patients allergic to A. simplex. METHODS: Twenty-eight adult patients, who had suffered an allergic episode caused by A. simplex were asked to follow one of the following three diets for a mean period of 13.16 months: a fish-free diet (diet 1; n = 19), a diet including fish frozen for more than 48 hours (diet 2; n = 9) and a diet with fresh fish (diet 3; n = 12). In all patients raw fish was excluded. Relapse rates and changes in total serum IgE and specific IgE to A. simplex were studied during the follow up. RESULTS: During the 13-month follow-up none of the patients developed anaphylaxis. Urticaria symptoms were present in 5.8 %, 11.1 % (n.s) and 33.3 % (p = 0.016) of patients following diets 1, 2 and 3, respectively. Total IgE decreased by 64 % (p < 0.05), 48 % (p < 0.05) and 39.4 % (p < 0.05), respectively. Specific IgE to A. simplex decreased by 50.7 % (p < 0.05), 54.1 % (p < 0.05) and 23.6 % (p < 0.05) after diets 1, 2 and 3, respectively. No statistically significant differences were found among the groups in variations in total and specific IgE. CONCLUSIONS: Patients allergic to A. simplex can eat fish that has been frozen at -20 C for 48 hours without risk of a severe allergic reaction. Long term decreases in specific and total IgE may not be good markers of eventual contact with A. simplex.
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| 10. |
- Wadi, Mohamed, 1963, et al.
(författare)
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Voltage Stability Analysis and Improvement of South-West Libya Electrical Power System, Part 1: Problem Identification
- 2008
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Ingår i: 43rd International Universities Power Engineering Conference, UPEC 2008; Padova; Italy; 1 September 2008 through 4 September 2008. - 9788889884096
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Konferensbidrag (refereegranskat)abstract
- Voltage stability has been of increasing importance in facing the rising risks of voltage collapses or system blackouts, and in trying to improve systems power quality, particularly in the relatively small and fast growing interconnected power systems of developing regions. This paper describes and identifies the recorded voltage fluctuations leading to some voltage collapses in the South-West Libyan electric power system, as a first step of handling this problem.
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