| 1. |
- Mårell, Lena, 1957-, et al.
(författare)
-
Psychological symptoms and self-image of patients with complaints attributed to dental restorative materials
- 2019
-
Ingår i: Clinical Oral Investigations. - : Springer Berlin/Heidelberg. - 1432-6981 .- 1436-3771. ; 23:6, s. 2805-2811
-
Tidskriftsartikel (refereegranskat)abstract
- Objectives: The aim was to study self-image and the level of psychological symptoms in patients with symptoms attributed to their dental restorative materials.Materials and methods: A questionnaire containing questions regarding dental and medical history was answered by 257 participants, one group with local oral symptoms only (LSO), and one group with multi-symptoms (M-S). A reference group was randomly selected from a research database at the Department of Psychology, Umeå University, Sweden. The self-image was assessed using the Structural Analysis of Social Behavior (SASB). Psychological symptoms such as somatization, depression, and anxiety were assessed using the Symptom Check List 90 (SCL-90) and the Global Severity Index (GSI) was used to determine the level of psychological symptoms.Results: SASB showed that the M-S group and the LSO-group scored significantly higher on the Bspontaneous^ and Bpositive self-image^ than the reference group. In the SCL-90, the M-S group scored significantly higher than the LSO-group and the references on the somatization subscales. On depression, anxiety, and the GSI scale, the M-S group scored significantly higher than the reference group.Conclusions: The two subgroups scored significantly higher on the SASB Spontaneous and Positive clusters which indicates that these patients have an excessively positive self-image, are very spontaneous and have an overconfidence in themselves compared to the reference group. In the M-S group there was a clear tendency to somatization, depression, and anxiety and they were more psychologically stressed than the reference group.Clinical relevance: Among the patients with illness attributed to their dental materials, the M-S-patients had a significantly higher level of general psychological distress and somatization than the control group which may lead to mental stress.
|
|
| 2. |
- Tillberg, Anders, et al.
(författare)
-
Changes in health over time in patients with symptoms allegedly caused by dental restorative materials
- 2005
-
Ingår i: Community Dentistry and Oral Epidemiology. - : John Wiley & Sons, Inc. - 0301-5661 .- 1600-0528. ; 33:6, s. 427-437
-
Tidskriftsartikel (refereegranskat)abstract
- Abstract – Objectives: In Sweden, many patients with symptoms allegedly caused by their dental materials have exchanged their restorations, but the effects of the exchange have been insufficiently investigated. Therefore, the aim of the study was to describe the change in health over time for these patients and the hypothesis was that the patients could be divided based on their symptoms and that the ability to recover differs between these groups. Furthermore, we also examined if other factors such as replacement of dental restorative materials and follow-up time had any impact on the perceived health status.Methods: A questionnaire was sent to 614 patients who had been referred to the School of Dentistry, Umeå, Sweden, with symptoms allegedly caused by dental restorative materials. The response rate was 55%.Results: The risk of having any further complaints was higher for patients with complex symptoms (P = 0.03) and these patients had exchanged their restorations to a significantly larger extent than the others (P = 0.03). The remaining complaints was more frequent among men (P = 0.02). Exchange of dental restorative materials had no significant impact on the ability to recover completely. However, the patients who had exchanged their restorations completely perceived a significantly larger alleviation of their symptoms than the others (P < 0.01), although the frequency of most of the symptoms had increased.Conclusions: Patients with complex symptoms had a more unfavorable long-term prognosis concerning persistent complaints than those with localized symptoms only. Furthermore, the results indicate that the patients might experience health improvements after removal of their dental restorative materials. The reason for this improvement, however, is unclear. Further analyses regarding other possible explanations than the ‘odontological/medical’ are needed.
|
|
| 3. |
- Andersson, Tilde, et al.
(författare)
-
Common skin bacteria protect their host from oxidative stress through secreted antioxidant RoxP
- 2019
-
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9
-
Tidskriftsartikel (refereegranskat)abstract
- Cutibacterium acnes is an abundant skin commensal with several proposed mutualistic functions. A protein with strong antioxidant activity was recently identified from the C. acnes secretome. This protein, termed RoxP, facilitated aerobic bacterial growth in vitro and ex vivo. As reducing events naturally occurred outside of the bacterial cell, it was further hypothesized that RoxP could also serve to modulate redox status of human skin. The biological function of RoxP was here assessed in vitro and in vivo, through oxidatively stressed cell cultures and through protein quantification from skin affected by oxidative disease (actinic keratosis and basal cell carcinoma), respectively. 16S rDNA amplicon deep sequencing and single locus sequence typing was used to correlate bacterial prevalence to cutaneous RoxP abundances. We show that RoxP positively influence the viability of monocytes and keratinocytes exposed to oxidative stress, and that a congruent concentration decline of RoxP can be observed in skin affected by oxidative disease. Basal cell carcinoma was moreover associated with microbial dysbiosis, characterized by reduced C. acnes prevalence. C. acnes's secretion of RoxP, an exogenous but naturally occurring antioxidant on human skin, is likely to positively influence the human host. Results furthermore attest to its prospective usability as a biopharmaceutical.
|
|
| 4. |
- Ankarberg, Emma, et al.
(författare)
-
Regionala skillnader i intag av persistenta organiska miljögifter hos förstföderskor i Uppsala, Göteborg och Lycksele
- 2007
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Under perioden 2000-2006 samlades bröstmjölk in från förstföderskor i Uppsala, Göteborg, Lund och Lycksele. Syftet med studien var att undersöka om det finns några regionala skillnader i halter av polyklorerade bifenyler (PCBer), klorerade pesticider (hexaklorbensen (HCB), ß-hexaklorocyklohexan (ß-HCH), oxyklordan, trans-nonaklor, DDT och DDT-metaboliter) och bromerade flamskyddsmedel (polybromerade difenyletrar (PBDE) och hexabromcyklododekan (HBCD)) i bröstmjölk. Resultaten visade att vissa signifikanta regionala skillnader i halter av de studerade substanserna förelåg. Skillnaderna var dock små, och inga säkra slutsatser om orsaker till skillnaderna kunde dras. Vissa regionala skillnader i halter av organiska miljögifter i livsmedel har också observerats. I en matkorgsstudie utförd 1999 (Darnerud et al. 2006) köptes livsmedel in i fyra olika städer i Sverige (Malmö, Göteborg, Uppsala och Sundsvall), och högre halter av ΣPCB, ΣDDT och ΣPBDE kunde ses i livsmedel från de södra delarna av Sverige i jämförelse med de norra. Denna tendens till nord-sydlig gradient observerades också i en senare matkorgsstudie som utfördes i samma städer 2005 (Ankarberg et al. 2006). Även den omfattande fiskundersökning som utfördes vid Livsmedelsverket 2000-2003 visade på regionala skillnader i halter i vissa fiskarter (NFA 2003). Studien som redovisas i denna rapport syftar till att genom intagsberäkningar undersöka om det finns regionala skillnader i exponering för organiska miljögifter (dioxiner, dioxinlika PCBer, PCB 153 och p,p´-DDE) från mat. Intagsberäkningarna grundas på en kostenkät, ifylld av mödrarna vid bröstmjölksinsamlingen, med frågor gällande konsumtionen av kött, fågel, fisk, mjölkprodukter, vegetabiliskt fett och ägg under året före graviditeten. Haltdata för de studerade substanserna i olika livsmedel hämtades från undersökningar och kontrollprogram vid Livsmedelsverket. De regionala jämförelser som gjordes inkluderade Uppsala, Göteborg och Lycksele. Den kostenkät som användes i Lund skiljde sig åt från de som användes i Uppsala, Göteborg och Lycksele. Resultaten från Lund kunde därför inte användas i denna undersökning. Trots vissa skillnader i konsumtionsmönster mellan de olika regionerna kunde inte några skillnader i intag av miljögifterna påvisas. Det beräknade medianintaget av dioxiner och dioxinlika PCBer varierade mellan 0,9 och 1,2 pg WHO-TEQ/kg kroppsvikt/dag, mellan 1,3 och 1,9 ng/kg kroppsvikt/dag för PCB 153 och mellan 2,5 och 3,5 ng/kg kroppsvikt/dag för 2 p,p´-DDE. Fisk och skaldjur bidrog till den största delen av intaget av WHO-TEQ (52-61 %). Enligt beräkningarna hade 14 % av kvinnorna intagsnivåer som överskred EU’s tolerabla veckointag för dioxiner och dioxinlika PCBer (14 pg WHO-TEQ/kg kroppsvikt/vecka). Livsmedelsverkets konsumtionsråd gällande fet fisk från Östersjön överskreds av 4 % av kvinnorna, och samtliga av dessa åtta individer fanns med bland dem som överskred TWI. Högexponerade individer karaktäriserades generellt av hög konsumtion av fisk och mjölkprodukter. Det finns inte någon tolerabel intagsnivå framtagen för icke dioxinlika PCBer (t.ex. PCB 153), så för dessa var det inte möjligt att göra några jämförelser. Ingen kvinna i studien överskred JECFA’s tolerabla dagliga intagsnivå för DDT-föreningar (10 μg/kg kroppsvikt/dag). En osäkerhet i de beräknade intagsnivåerna är att det är mycket svårt att minnas och korrekt ange sin konsumtion av olika livsmedel under ett år tillbaka. Dessutom är det troligt att konsumtionen av lax från Östersjön är överskattad eftersom det är mycket ovanligt med sådan fisk i butik. De haltdata som använts i beräkningarna är medel- och medianhalter som kommer från olika analysprojekt vid Livsmedelsverket. Dessa halter kan skilja sig åt från de verkliga nivåerna i de livsmedel som kvinnorna konsumerat. Dessutom användes samma haltdata för samtliga kvinnor, och hänsyn togs alltså inte till eventuella regionala skillnader i livsmedelshalter. Trots dessa osäkerheter tyder resultaten på att svenska förstföderskor från olika delar av landet har exponerats för liknande nivåer av de studerade föreningarna. En korrelationsanalys kunde inte påvisa några samband mellan de beräknade intagen av PCB 153 och p,p´-DDE och nivåerna av dessa substanser i bröstmjölk. Detta beror sannolikt till viss del på att bröstmjölkshalterna av persistenta organiska miljögifter speglar den totala exponeringen under livstiden, och inte bara exponeringen under det senaste året.
|
|
| 5. |
- Bergdahl, Anders
(författare)
-
Altered vascular responses in experimental congestive heart failure
- 1999
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Congestive heart failure is accompanied with increased circulatory levels of several vasoregulatory neurotransmitters and hormones which affect the outcome of this disease. In this thesis the blood levels of several vasoactive peptides in humans with congestive heart failure and their effects on the vasculature by the use of an animal model of heart failure were studied. Patients with congestive heart failure had increased levels of noradrenaline (NA), adrenaline (A), neuropeptide Y, atrial natriuretic peptide (ANP) and antidiuretic hormone. The increased levels of ANP and NA correlated to the survival time in patients with congestive hart failure. Increased levels of NA and A were also found in the animal heart failure model. The passive elastic and active contractile capacity of small mesenteric arteries were attenuated in heart failure animals. Ca2+-mediated and KCl -induced contractions were attenuated in heart failure vessels, an effect due to alterations of a-adrenoceptors. The contractile responses of isolated vessel segments to agonists of the sympathetic nervous system were altered, especially the a2- adrenergic receptor, which mediated an attenuated response in several vascular beds, both in vivo and in vitro, in heart failure animals. The sympathetic co-transmitter neuropeptide Y (NPY) enhanced the response to NA in human omental arteries via the Y1 receptor and this receptor was involved in the modulation of the response to endothelin (ET) -1, U46619 and 5-HT in heart failure vessels. NPY-induced potentiation of ET seem to be mediated by a receptor different from the NPY Y1 type. Endothelin was found to be less potent in large conductance arteries in heart failure while the reverse was noted in small mesenteric arteries. The response mediated through the smooth muscle endothelin B receptor was altered since inhibition of this receptor in endothelium-denuded arteries induced a leftward shift of the concentration-response curve in heart failure but not in control animals. The dilatory response to calcitonin gene-related peptide was attenuated in several arterial beds in heart failure rats. It is concluded that the animal model is suitable for studying the effects of neurotransmitters in heart failure and correlates with findings in humans. Further, several receptor alterations are induced which might influence the outcome of this condition.
|
|
| 6. |
- Bergdahl, Anders, et al.
(författare)
-
Neuropeptide Y potentiates noradrenaline-induced contraction through the neuropeptide Y Y1 receptor
- 1996
-
Ingår i: European Journal of Pharmacology. - 1879-0712. ; 316:1, s. 59-64
-
Tidskriftsartikel (refereegranskat)abstract
- To elucidate which neuropeptide Y receptor subtype is responsible for the neuropeptide Y-induced potentiation of the noradrenaline-evoked contraction in human omental arteries we used antisense oligodeoxynucleotide (Antisense), the new selective neuropeptide Y Y1 receptor antagonist, BIBP3226 {(R)-N2-(diphenylacetyl)-N-[(4-hydroxyphenyl) methyl]-D-arginine-amide} and the reverse transcriptase-polymerase chain reaction (RT-PCR). Neuropeptide Y significantly potentiated the noradrenaline-induced contraction in non-incubated vessels (pEC50 6.4 +/- 0.2 vs. 5.9 +/- 0.2) and in vessels incubated with 1 microM Sense oligodeoxynucleotide (Sense) (pEC50 6.0 +/- 0.1 vs. 5.6 +/- 0.2). In vessels incubated with 1 microM Antisense the potentiating effect of neuropeptide Y was completely abolished. BIBP3226 (1 microM) inhibited the neuropeptide Y-induced potentiation in human omental arteries (pEC50 5.8 +/- 0.3 vs. 6.4 +/- 0.2). Finally, messenger RNA for the neuropeptide Y Y1 receptor was detected using RT-PCR. On the basis of our results we conclude that the neuropeptide Y-induced potentiation of the noradrenaline-induced contraction is mediated by the neuropeptide Y Y1 receptor.
|
|
| 7. |
- Bergdahl, Anders, et al.
(författare)
-
The use of DFA/DFAA Analysis for early start up of Assembly System Design
- 2005
-
Ingår i: International Forum on Design for Manufacture and Assembly. - Providence - Warwick, Rhode Island, USA.
-
Konferensbidrag (refereegranskat)abstract
- Assembly system design and development is still characterized by uncertainty resulting in increased lead-times in the design and development process and ineffective assembly system solutions. One important reason for this situation is that companies do not use methods for assembly system design and development in the extent needed, simply because there are no methods applicable. Methods developed by academia are still too complicated and not distinct enough, in quantifiable means, concerning the link to assembly system design. In this research four case studies in four different companies have been executed with the objective to find out more about how industry handles assembly system design and if there are possibilities for improvement. The findings in the case studies unambiguously point out that there is a need for a guideline supporting the assembly system design, development and re-design phases. The case studies do also indicate that the information from DFA/DFA2 analysis, valuable for the assembly system design process, is not used within the subsequent assembly system design phase. An assembly system design guideline that uses information and results from the DFA work, together with internal and external demands, would facilitate the assembly system design and development process. This paper will introduce a framework for such a method.
|
|
| 8. |
- Bergdahl, Basti, et al.
(författare)
-
Engineering yeast hexokinase 2 for improved tolerance toward xylose-induced inactivation.
- 2013
-
Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 8:9
-
Tidskriftsartikel (refereegranskat)abstract
- Hexokinase 2 (Hxk2p) from Saccharomyces cerevisiae is a bi-functional enzyme being both a catalyst and an important regulator in the glucose repression signal. In the presence of xylose Hxk2p is irreversibly inactivated through an autophosphorylation mechanism, affecting all functions. Consequently, the regulation of genes involved in sugar transport and fermentative metabolism is impaired. The aim of the study was to obtain new Hxk2p-variants, immune to the autophosphorylation, which potentially can restore the repressive capability closer to its nominal level. In this study we constructed the first condensed, rationally designed combinatorial library targeting the active-site in Hxk2p. We combined protein engineering and genetic engineering for efficient screening and identified a variant with Phe159 changed to tyrosine. This variant had 64% higher catalytic activity in the presence of xylose compared to the wild-type and is expected to be a key component for increasing the productivity of recombinant xylose-fermenting strains for bioethanol production from lignocellulosic feedstocks.
|
|
| 9. |
- Bergdahl, Ingvar A., et al.
(författare)
-
Lead binding to delta-aminolevulinic acid dehydratase (ALAD) in human erythrocytes
- 1997
-
Ingår i: Basic and Clinical Pharmacology and Toxicology. - : Wiley. - 0901-9928. ; 81:4, s. 153-158
-
Tidskriftsartikel (refereegranskat)abstract
- Over 99% of the lead present in blood is usually found in erythrocytes. To investigate the nature of this selective accumulation of lead in erythrocytes, the specific binding of lead to proteins in human erythrocytes was studied using liquid chromatography coupled to inductively coupled plasma mass spectrometry (LC-ICP-MS). The principal lead-binding protein had a mass of approximately 240 kDa, and adsorption to specific antibodies showed that protein was delta-aminolevulinic acid dehydratase (ALAD). Thus, the previous notion that lead in erythrocytes was bound primarily to haemoglobin has to be revised. Furthermore, in lead-exposed workers, the percentage of lead bound to ALAD was influenced by a common polymorphism in the ALAD gene. Specifically, in seven carriers of the ALAD2 allele, 84% of the protein-bound lead recovered was bound to ALAD compared to 81% in seven homozygotes for the ALAD1 allele whose erythrocytes were matched for blood-lead concentration. The small difference was statistically significant in Wilcoxon matched-pairs signed-rank test (P = 0.03). No ALAD allele-specific difference in ALAD-bound lead was found among 20 unexposed controls. Perhaps the difference in ALAD-bound lead can provide an explanation for the previously reported finding of higher blood-lead levels among carriers of the ALAD2 allele than among ALAD1 homozygotes in lead-exposed populations.
|
|
| 10. |
- Bergdahl, Lars, 1943, et al.
(författare)
-
Förankringen till en vågenergiboj i Kattegatt
- 1981
-
Ingår i: Efaringer fra dagens forankringssystemer og krav til morgendagens. Noges sivilingenjörers forening. ; , s. 18-
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Sommaren 1979 genomfördes i sjön Lygnern
|
|
