| 1. |
- Sellberg, Felix, et al.
(författare)
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Composition of growth factors and cytokines in lysates obtained from fresh versus stored pathogen-inactivated platelet units
- 2016
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Ingår i: Transfusion and apheresis science. - : Elsevier BV. - 1473-0502 .- 1878-1683. ; 55:3, s. 333-337
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Tidskriftsartikel (refereegranskat)abstract
- Background: Platelet lysate is a readily available source of growth factors, and other mediators, which has been used in a variety of clinical applications. However, the product remains poorly standardized and the present investigation evaluates the composition of platelet lysate obtained from either fresh or stored pathogen-inactivated platelet units.Materials and Methods: Platelet pooled units (n = 10) were obtained from healthy blood donors and tested according to standard procedures. All units were pathogen inactivated using amotosalen hydrochloride and UVA exposure. Platelet lysate was subsequently produced at two separate time-points, either from fresh platelet units or after 5 days of storage, by repeated freeze-thaw cycles. The following mediators were determined at each time point: EGF, FGF-2, VEGF, IGF-1, PDGF-AB/BB, BMP-2, PF4, TGF-beta isoform 1, IL-1(i, IL-2, IL-6, IL-10, IL-12p70, 1L-17A, TNF-alpha, and IFN-gamma.Results: The concentration of growth factors and cytokines was affected by time in storage. Notably, TGF-beta, PDGF-AB/BB, and PF4 showed an increase of 27.2% (p < 0.0001), 29.5% (p = 0.04) and 8.2% (p = 0.0004), respectively. A decrease was seen in the levels of IGF-1 and FGF-2 with 22% (p = 0.041) and 11% (p = 0.01), respectively. Cytokines were present only in very low concentrations and all other growth factors remained stable with time in storage.Conclusion: The composition of mediators in platelet lysate obtained from pathogen inactivated platelet units differs when produced from fresh and stored platelet units, respectively. This underscores the need for further standardization and optimization of this important product, which potentially may influence the clinical effects.
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| 2. |
- Berglund, Daniel, et al.
(författare)
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Comparative life-cycle assessment for renovation methods of waste water sewerage systems for apartment buildings
- 2018
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Ingår i: Journal of Building Engineering. - : Elsevier. - 2352-7102. ; 19, s. 98-108
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Tidskriftsartikel (refereegranskat)abstract
- This comparative life-cycle assessment highlights three main alternatives for renovation of waste water sewerage: pipe replacement, cured- in- place pipe (CIPP) lining (also called sliplining) and renovation by coatings. The functional unit of this study is a six-story block house that was built in 1960 and has 29 apartments. The characterized results of environmental impacts display an advantage for CIPP-lining over pipe replacement in 14 of the 18 studied impact categories. Regarding those categories in which impacts were comparatively large, when looking at the average impact from a European citizen according to the ReCiPe methodology for life cycle inventory list, pipe replacement has greater impacts than CIPP-lining. In general, the impacts of pipe replacement are related to new tiles, expanded polyester cement, the screed, and the material for waterproofing, as well as the electricity needed for drying the structure. The CIPP-lining method displays higher impacts than pipe replacement in just four categories. These impacts are, to a large extent, caused by the use of consumables such as gloves and cotton cloths. From an LCA-perspective, the study shows that the CIPP and coatings relining methods have advantages over pipe replacement under the condition that the technical lifetime is the same for these methods. Still, the uncertainty of service life, as well as Bisphenol A (BPA) emissions, remain as issues of concern for further study. There are also other differences among the alternatives that ultimately influence a property owner's choice of method, such as costs, inconvenience for the residents, renewal of bathroom interiors, and the way in which the property owner values the alternative technologies.
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| 3. |
- Berglund, Daniel, et al.
(författare)
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Relining eller rörbyte - en jämförelse med LCA
- 2018
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Rapport (populärvet., debatt m.m.)abstract
- Denna jämförande livscykelbedömning belyser tre huvudalternativ för renovering av avloppsrör; rörbyte, relining med rörfoder (strumpmetoden, CIPP-lining) som även kallas sliplining och renovering icke-bärande plastbeläggningar. Den funktionella enheten i denna studie är ett sex våningar högt punkthus som byggdes 1960 och som har 29 lägenheter. De redovisade resultaten av miljöpåverkan visar att strumpmetoden har fördelar framför rörbyte i 14 av de 18 undersökta kategorierna. När det gäller de kategorier där påverkningarna var förhållandevis stora, jämfört med en genomsnittliga europeisk medborgares årliga miljöbelastning, enligt ReCiPe-metoden, så har renoveringmetoden rörbyte större påverkan än strumpmetoden. Påverkningarna av rörbyte är främst relaterade till nya klinkerplattor, EPS-cement, avjämningsmassor och materialet för tätskikt, och även den el som behövs för att torka byggnaden. Strumpmetoden visar högre påverkan än rörbyte i fyra kategorier. Dessa påverkningar orsakas till stor del av användningen av förbrukningsmaterial som handskar och bomullstrasor. Ur ett LCA-perspektiv visar studien att strumpmetoden och icke-bärande beläggningsreningsmetoder har fördelar framför rörbyte under förutsättning att den tekniska livslängden är densamma. Osäkerheten om livslängden och även frågan om utsläpp av Bisphenol A (BPA) är aspekter som behöver fortsatta studier. Det finns också andra faktorer som till sist påverkar vilken metod för stamrenovering som en fastighetsägare väljer; kostnader, besvär för de boende, behov av renovering av badrum till sist hur fastighetsägaren själv värderar de olika alternativen.
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| 4. |
- Berglund, Ellinor, et al.
(författare)
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A smartphone application for dispatch of lay responders to out-of-hospital cardiac arrests.
- 2018
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Ingår i: Resuscitation. - : Elsevier BV. - 1873-1570 .- 0300-9572. ; 126, s. 160-165
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Tidskriftsartikel (refereegranskat)abstract
- Dispatch of lay volunteers trained in cardiopulmonary resuscitation (CPR) and equipped with automated external defibrillators (AEDs) may improve survival in cases of out-of-hospital cardiac arrest (OHCA). The aim of this study was to investigate the functionality and performance of a smartphone application for locating and alerting nearby trained laymen/women in cases of OHCA.A system using a smartphone application activated by Emergency Dispatch Centres was used to locate and alert laymen/women to nearby suspected OHCAs. Lay responders were instructed either to perform CPR or collect a nearby AED. An online survey was carried out among the responders.From February to August 2016, the system was activated in 685 cases of suspected OHCA. Among these, 224 cases were Emergency Medical Services (EMSs)-treated OHCAs (33%). EMS-witnessed cases (n=11) and cases with missing survey data (n=15) were excluded. In the remaining 198 OHCAs, lay responders arrived at the scene in 116 cases (58%), and prior to EMSs in 51 cases (26%). An AED was attached in 17 cases (9%) and 4 (2%) were defibrillated. Lay responders performed CPR in 54 cases (27%). Median distance to the OHCA was 560m (IQR 332-860m), and 1280m (IQR 748-1776m) via AED pick-up. The survey-answering rate was 82%.A smartphone application can be used to alert CPR-trained lay volunteers to OHCAs for CPR. Further improvements are needed to shorten the time to defibrillation before EMS arrival.
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| 5. |
- Engström, Gunnar, et al.
(författare)
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Blood pressure increase and incidence of hypertension in relation to inflammation-sensitive plasma proteins.
- 2002
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Ingår i: Arteriosclerosis, Thrombosis and Vascular Biology. - 1524-4636. ; 22:12, s. 2054-2058
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Tidskriftsartikel (refereegranskat)abstract
- Objective— The reasons for the relationship between inflammation-sensitive plasma proteins (ISPs) and incidence of cardiovascular diseases are poorly understood. This study explored the hypothesis that ISPs are associated with future hypertension and age-related blood pressure increase. Method and Results— Blood pressure and plasma levels of fibrinogen, {alpha}1-antitrypsin, haptoglobin, ceruloplasmin, and orosomucoid were determined in 2262 healthy men aged 35 to 50 years, initially without treatment for hypertension. The cohort was re-examined after 15.7 (±2.2) years. Incidence of hypertension and blood pressure increase was studied in relation to number of elevated proteins (ie, in the top quartile) at baseline. Among men without treatment for hypertension at follow-up, mean (±SD) increase in systolic blood pressure was 18.8±17, 19.2±17, 19.3±17, and 22.1±18 mm Hg, respectively, for men with 0, 1, 2, and >=3 elevated proteins (P for trend=0.02, adjusted for confounders). The corresponding values for pulse pressure increase was 15.5±14, 15.8±14, 17.4±14, and 17.8±15 mm Hg, respectively (P=0.02). Incidence of hypertension (>=160/95 mm Hg or treatment) and future blood pressure treatment showed similar associations with ISPs. Increase in diastolic blood pressure showed no association with ISPs. Conclusions— Plasma levels of ISPs are associated with a future increase in blood pressure. This could contribute to the relationship between ISP levels and cardiovascular disease.
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| 6. |
- Engström, Gunnar, et al.
(författare)
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Plasma levels of complement C3 is associated with development of hypertension: a longitudinal cohort study.
- 2007
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Ingår i: Journal of Human Hypertension. - : Springer Science and Business Media LLC. - 1476-5527 .- 0950-9240. ; 21, s. 276-282
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Tidskriftsartikel (refereegranskat)abstract
- Hypertension has been associated with raised plasma levels of complement factor 3 and 4 (C3 and C4). The nature of this association is unclear. This population-based longitudinal study explored whether C3 or C4 is associated with development of hypertension. Blood pressure and plasma levels of C3 and C4 were determined in 2178 healthy men, aged 35-50 years, initially without treatment for hypertension. Incidence of hypertension and blood pressure increase over 15.7 (+/- 2.2) years follow-up was studied in relation to C3 and C4 at baseline. Among men with initially normal blood pressure (<160/95 mm Hg), incidence of hypertension (>= 160/95 mm Hg or treatment) was 32, 42, 37 and 47%, respectively, for men with C3 in the first, second, third and fourth quartile (trend: P = 0.001). This relationship remained significant after adjustment for confounding factors. Among men without blood pressure treatment, systolic BP increase (mean + standard error, adjusted for age, initial blood pressure and follow-up time) was 17.5 + 0.8, 19.6 + 0.9, 19.8 + 0.8 and 20.8 + 0.8 mm Hg, respectively, in the C3 quartiles (trend: P = 0.004). C3 was not associated diastolic blood pressure at follow-up. Although C4 was associated with blood pressure at the baseline examination, there was no relationship between C4 and development of hypertension or future blood pressure increase. It is concluded that C3 in plasma is associated with future blood pressure increase and development of hypertension.
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| 7. |
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| 8. |
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| 9. |
- Klaff, Rami, et al.
(författare)
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Clinical presentation and predictors of survival related to extent of bone metastasis in 900 prostate cancer patients
- 2016
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Ingår i: Scandinavian journal of urology. - : TAYLOR & FRANCIS LTD. - 2168-1805 .- 2168-1813. ; 50:5, s. 352-359
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Tidskriftsartikel (refereegranskat)abstract
- Objective: The aim of this study was to investigate the impact of bone metastasis on survival and quality of life (QoL) in men with hormone-naive prostate cancer. Materials and methods: The study included 900 patients from a randomized trial (No. 5) by the Scandinavian Prostate Cancer Group, comparing parenteral oestrogen with total androgen blockade. Extent of bone metastasis was categorized according to a modified Soloway score: score 1, n=319; score 2, n = 483; and score 3, n = 98 patients. The primary outcome measurements were mean differences in QoL and overall survival. Results: QoL rating scales showed a decrease with increasing extent of bone metastasis (p amp;lt; 0.001). The mean global health status decreased from 64.4 to 50.5 for Soloway score 1 and 3, respectively. Following adjustment for performance status, analgesic consumption, grade of malignancy, alkaline phosphatase, prostate-specific antigen, haemoglobin and global health status, Soloway score 2 and 3 had a 47% [hazard ratio (HR) 1.47, 95% confidence interval (CI) 1.21-1.80] and 78% (HR 1.78 95%, CI 1.32-2.42) increased mortality, respectively, compared to Soloway score 1. Independent predictive factors of mortality were assessed. Conclusions: Patient grouping based on three categories of extent of bone metastasis related to performance status, haemoglobin and global health status at presentation, as independent predictors of mortality, may provide improved accuracy of prognosis.
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| 10. |
- Nilsson, Peter, et al.
(författare)
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The enigma of increased non-cancer mortality after weight loss in healthy men who are overweight or obese.
- 2002
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Ingår i: Journal of Internal Medicine. - : Wiley. - 1365-2796 .- 0954-6820. ; 252:1, s. 70-78
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Tidskriftsartikel (refereegranskat)abstract
- Objective. To study effects on non-cancer mortality of observational weight loss in middle-aged men stratified for body mass index (BMI), taking a wide range of possible confounders into account. Design. Prospective, population based study. Setting. Male population of Malmö, Sweden. Participants. In all 5722 men were screened twice with a mean time interval of 6 years in Malmö, southern Sweden. They were classified according to BMI category at baseline (<21, 22-25, overweight: 26-30, and obesity: 30+ kg m-2) and weight change category until second screening (weight stable men defined as having a baseline BMI ± 0.1 kg m-2 year-1 at follow-up re-screening). Main outcome measures. Non-cancer mortality calculated from national registers during 16 years of follow-up after the second screening. Data from the first year of follow-up were excluded to avoid bias by mortality caused by subclinical disease at re-screening. Results. The relative risk (RR; 95% CI) for non-cancer mortality during follow-up was higher in men with decreasing BMI in all subgroups: RR 2.64 (1.46-4.71, baseline BMI <21 kg m-2), 1.39 (0.98-1.95, baseline BMI 22-25 kg m-2), and 1.71 (1.18-2.47, baseline BMI 26+ kg m-2), using BMI-stable men as reference group. Correspondingly, the non-cancer mortality was also higher in men with increasing BMI, but only in the obese group (baseline BMI 26+ kg m-2) with RR 1.86 (1.31-2.65). In a subanalysis, nonsmoking obese (30+ kg m-2) men with decreased BMI had an increased non-cancer mortality compared with BMI-stable obese men (Fischer's test: P=0.001). The mortality risk for nonsmoking overweight men who increased their BMI compared with BMI-stable men was also significant (P=0.006), but not in corresponding obese men (P=0.094). Conclusions. Weight loss in self-reported healthy but overweight middle-aged men, without serious disease, is associated with an increased non-cancer mortality, which seems even more pronounced in obese, nonsmoking men, as compared with corresponding but weight-stable men. The explanation for these observational findings is still enigmatic but could hypothetically be because of premature ageing effects causing so-called weight loss of involution.
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