| 1. |
- Penell, Johanna Christina, et al.
(författare)
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Concentrations of nine endogenous steroid hormones in 70-year-old men and women
- 2021
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Ingår i: Endocrine Connections. - : Bioscientifica. - 2049-3614. ; 10:5, s. 511-520
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Tidskriftsartikel (refereegranskat)abstract
- ObjectivesCirculating concentrations of endogenous steroids have systemic implications on health in elderly. However, population-based age- and ethnicity-specific data are scarce. The aim was to report sex-specific plasma concentrations of endogenous sex and adrenal steroids in elderly Swedish Caucasians, to examine the impact of BMI and to present concentrations in apparently healthy subjects.MethodsA population-based observational study of 70-year olds, including 684 community-dwelling men and women enrolled in the PIVUS study, Sweden. Median plasma concentrations were determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for pregnenolone, 17-hydroxypregnenolone, 17-hydroxy-progesterone, 11-deoxycortisol, DHEA, androstenedione, testosterone, estrone and estradiol.ResultsPlasma concentrations were significantly higher in men (n = 452) than in women (n = 232) for estradiol: median 61.3 pmol/L (95% CI, 11.4, 142.7) vs 18.4 (4.0, 127.3), for estrone: 92.8 (33.3, 206) vs 71.6 (17.8, 209) pmol/L, and for testosterone 13.8 (5.7, 28.0) vs 0.7 (0.2, 2.0) nmol/L. Higher concentrations of estrone and estradiol were observed in obese than non-obese women. Compared to non-obese men, obese men had lower concentrations of testosterone and its precursors: 17-hydroxypregnenolone, 17-hydroxyprogesterone, androstenedione and DHEA. The subgroup of apparently healthy individuals had median values > 20% lower for estrone and estradiol in women but slightly higher for testosterone in both sexes.ConclusionsConcentrations of estradiol, estrone and testosterone were higher in 70-year-old men than in women. BMI associated positively to estradiol and estrone in women and negatively to testosterone in men. Apparently healthy women had lower median concentrations of estradiol and estrone and men had higher median testosterone compared to all individuals.
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| 2. |
- Ahnlund, Jonas, et al.
(författare)
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An alarm reduction application at a district heating plant
- 2003
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Ingår i: EFTA 2003. 2003 IEEE Conference on Emerging Technologies and Factory Automation. Proceedings (Cat. No.03TH8696). - 0780379373 ; , s. 187-190
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Konferensbidrag (refereegranskat)abstract
- The proper handling of alarms is crucial to any automated process control. In practice, many alarms are only distractive and do not represent a fault situation. In an ongoing project a computerized tool is applied, that aims to remove such nuisance alarms, a so-called alarm cleanup. This is a general, systematic approach that takes advantage of the control system's built-in functions, and is a first step to an enhanced overall alarm situation. In a first sharp case study, a significant reduction of the alarms was achieved at a biofueled district heating plant
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| 3. |
- Ahnlund, Jonas, et al.
(författare)
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Intelligent reduction of nuisance alarms in process control
- 2003
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Ingår i: Notes SAIS/SSLS Joint Workshop.
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Konferensbidrag (refereegranskat)abstract
- The proper handling of alarms is crucial to automated process control. Fault Detection and Isolation techniques have been widely researched to determine such alarms. In practice, many alarms are only distractive and do not represent a fault situation. Here a toolbox, that uses an expert system called LARA, is proposed that aims to reduce the occurrence of so-called nuisance alarms. It is based on a number of signal classifiers and can be easily extended with metrical abnormality predictors. In a first prototype, a significant reduction of the alarms in a biofueled District Heating Plant has been achieved.
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| 4. |
- Ahnlund, Jonas, et al.
(författare)
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Rule-based reduction of alarm signals in industrial control
- 2003
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Ingår i: Journal of Intelligent & Fuzzy Systems. - 1064-1246. ; 14:2, s. 73-84
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Tidskriftsartikel (refereegranskat)abstract
- The proper handling of alarms is crucial to any automated process control. In practice, many alarms are only distractive and do not represent a potentially dangerous situation. This paper presents a methodology and a computerized tool that aims to remove such nuisance alarms, a so-called alarm cleanup. This is a general, systematic approach that takes advantage of the control system's built-in functions, and is a first step to an improved overall alarm situation. By the strong reduction of the alarm count, the efficient construction of fault diagnosis and isolation models becomes feasible. In a typical case study, the number of alarms received at the remote control room of an operational bio-fueled District Heating Plant was effectively reduced by 83%.
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| 5. |
- Bergquist, Tord, et al.
(författare)
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Alarm reduction in industrial process control
- 2003
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Ingår i: EFTA 2003. 2003 IEEE Conference on Emerging Technologies and Factory Automation. Proceedings (Cat. No.03TH8696). - 0780379373 ; 2, s. 58-65
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Konferensbidrag (refereegranskat)abstract
- The proper handling of alarms is crucial to automated process control. In practice, many alarms are only distractive and do not represent a fault situation. Here, a toolbox is proposed that aims to reduce the occurrence of the so called nuisance alarms. It is a general computerized tool that takes advantage of the control system's built-in functions, and is a first step to an enhanced overall alarm situation. In the first prototype, a significant reduction of the alarms in a biofueled district heating plant has been achieved
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| 6. |
- Bergquist, Tord, et al.
(författare)
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Signal processing and alarm handling in process control
- 2003
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Ingår i: Proceedings of the 16th European Conference on Circuit Theory and Design, ECCTD'03. - 8388309951 ; , s. 72-369
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Konferensbidrag (refereegranskat)abstract
- The proper handling of alarms is crucial to automated process control. In practice, many alarms are only distractive and do not represent a fault situation. Here a toolbox, that uses an expert system called LARA, is proposed that aims to reduce the occurrence of so-called nuisance alarms. It is based on a signal classification scheme to propose suitable alarm handling algorithms according to the signals behavior. In a first prototype, a significant reduction of the alarms in a biofueled District Heating Plant has been achieved
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| 7. |
- Bergquist, Tord, et al.
(författare)
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Using correlation in MFM model design and validation
- 2004
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Ingår i: Proceedings AILS-04 Workshop. - 1650-1276.
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Konferensbidrag (refereegranskat)abstract
- Industrial process signals do affect one other. However, causal dependencies, or corre-lation, and thereby consequences of control actions cannot always be understood by the operator, which may lead to accidents. Models explain dependencies and are mostly built on process knowledge and blueprints, while causalities are visible in the measurements and ought to be used to increase process under-standing. In this paper we will discuss why the cross-correlation technique is insufficient for causality detection in industrial processes and we will present a new method that, for instance, can be used to validate the design of multilevel flow models. Keywords: Correlation, multilevel flow model, fault detection, alarm analysis.
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| 8. |
- Hanrieder, Jörg, 1980, et al.
(författare)
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Proteomic analysis of human follicular fluid using an alternative bottom-up approach.
- 2008
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Ingår i: Journal of proteome research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 7:1, s. 443-9
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Tidskriftsartikel (refereegranskat)abstract
- Human follicular fluid (hFF) is the in vivo environment of oocytes during follicular maturation in the ovaries. It contains a huge variety of compounds such as, e.g., proteins that might play an important role in follicular development and oocyte growth. Previous proteomic studies on follicular fluid have isolated and already identified a certain number of proteins. Nevertheless, only a small part of proteins present in follicular fluid have been covered so far and a large number have still not been identified. Therefore, the need for new, more resolving, and sensitive approaches in proteome research is evident. We utilized a proteomic setup based on in solution isoelectric focusing (IEF) and reversed-phase nanoliquid chromatography coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (nano-LC MALDI TOF/TOF MS) for in depth protein analysis of human follicular fluid samples of patients undergoing controlled ovarian hyper stimulation (COH) for in vitro fertilization therapy (IVF). This approach led to the significant identification of 69 proteins, where 32 have not been reported before to be found in human follicular fluid with proteomic methods. Among these findings, at least two relevant compounds essentially involved in hormone secretion regulation during the folliculogenetic process were identified: sex hormone binding globulin (SHBG) and inhibin A (INHA). To confirm these results, both proteins were further validated by immunoassays.
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| 9. |
- Kushnir, Mark M., et al.
(författare)
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Exploratory study of the association of steroid profiles in stimulated ovarian follicular fluid with outcomes of IVF treatment
- 2016
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Ingår i: Journal of Steroid Biochemistry and Molecular Biology. - : Elsevier BV. - 0960-0760 .- 1879-1220. ; 162, s. 126-133
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Forskningsöversikt (refereegranskat)abstract
- Steroid concentrations in stimulated follicular fluid (sFF) samples have been linked to the quality of oocytes used in IVF treatments. Most of the published studies focused on evaluating the association of the IVF outcomes with only a few of the steroids, measured by immunoassays (IA). We performed a treatment outcome, prospective cohort study using stimulated FF sampled from 14 infertile women undergoing IVF treatment; single oocyte was used per IVF cycle. Fourteen endogenous steroids were analyzed in 22 ovarian follicle aspirations, which corresponded to the embryos used in the IVF. Ten oocytes were associated with live birth (LB) and 12 with no pregnancy (NP). Steroids were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods. Differences in distribution of concentrations in association with the pregnancy outcome (LB or NP), and receiver operating characteristic (ROC) curves analysis were performed for the entire cohort and for within-women data. The predominant androgen and estrogen in stimulated sFF were androstenedione (A4) and estradiol (E2), respectively. Lower concentrations of pregnenolone (Pr), lower ratios of A4/ dehydroepiandrosterone (DHEA), testosterone (Te)/DHEA, and greater ratios of E2/Te, and estrone/A4 were observed in sFF samples associated with LB. Among the oocytes associated with NP, in four out of 12 samples total concentration of androgens was above the distribution of the concentrations in the oocytes corresponding to the LB group. Observations of the study indicated increased consumption of precursors and increased biosynthesis of estrogens in the follicles associated with LB. Our data suggest that potentially steroid profiles in sFF obtained during oocyte retrieval may serve as biomarkers for selection of the best embryo to transfer after IVF.
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| 10. |
- Kushnir, Mark M., et al.
(författare)
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Protein and Steroid Profiles in Follicular Fluid after Ovarian Hyperstimulation as Potential Biomarkers of IVF Outcome
- 2012
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 11:10, s. 5090-5100
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Tidskriftsartikel (refereegranskat)abstract
- Controlled ovarian hyperstimulation is performed to assist with generation of multiple mature oocytes for use in in vitro fertilization (IVF). The goal of our study was to evaluate differences in protein and steroid profiles in ovarian follicular fluid (hFF) samples obtained during oocy-te retrieval from women undergoing IVF treatment and to identify physiological pathways associated with the proteins. The hFF samples were depleted of abundant proteins, fractionated by ultrafiltration, digested, and analyzed by nano-LC QTOF. Concentrations of 15 endogenous steroids were determined in the samples using LC-MS/MS methods. The total number of proteins identified in the samples was 75, of which 4, 7, and 2 were unique to the samples from women with viable pregnancy, miscarriage, and no pregnancy, respectively. Identified proteins were associated with the acute response signaling, coagulation system, intrinsic and extrinsic prothrombin activation, complement system, neuroprotective role of THOP1, FXR/RXR activation, role of tissue factor, and growth hormone pathways. A greater number of proteins associated with biosynthesis was found in hFF samples corresponding to the oocytes resulting in pregnancy. The abundance of seven proteins was found to be associated with steroidogenesis. The obtained data will contribute to better understanding of the pathogenesis and development of noninvasive markers for assessment of oocytes viability.
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