| 1. |
|
|
| 2. |
- Duong-Thi, Minh-Dao, et al.
(författare)
-
Comparison of weak affinity chromatography and surface plasmon resonance in determining affinity of small molecules
- 2014
-
Ingår i: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 461, s. 57-59
-
Tidskriftsartikel (refereegranskat)abstract
- In this study, we compared affinity data from surface plasmon resonance (SPR) and weak affinity chromatography (WAC), two established techniques for determination of weak affinity (mM-mu M) small molecule-protein interactions. In the current comparison, thrombin was used as target protein. In WAC the affinity constant (K-D) was determined from retention times, and in SPR it was determined by Langmuir isotherm fitting of steady-state responses. Results indicate a strong correlation between the two methods (R-2 = 0.995, P < 0.0001). (C) 2014 Elsevier Inc. All rights reserved.
|
|
| 3. |
- Stashans, Arvids, et al.
(författare)
-
Theoretical study of lithium intercalation in rutile and anatase
- 1996
-
Ingår i: Physical Review B Condensed Matter. - 0163-1829 .- 1095-3795. ; 53:1, s. 159-170
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Motivated by recent developments concerning coloration and energy storage in lithium intercalated nanostructural TiO2, quantum chemical Hartree-Fock calculations have been carried out to study lithium atom intercalation in rutile and anatase. Equilibrium geometries and effective atomic charges were obtained for the rutile (110) and anatase (101) clean surfaces. Li-induced local one-electron energy levels were found in the gap between the upper valence band and the conduction band and could be attributed to Ti3+ states. The absorption energies obtained are compared with available experimental data. The equilibrium positions of the Li atom and its surrounding host atoms have been calculated for both structures. The results predict a higher possibility of lithium intercalation in the anatase structure than in rutile.
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
- Bergström, Dan, et al.
(författare)
-
Effects of raw material particle size distribution on the characteristics of Scots pine sawdust fuel pellets
- 2008
-
Ingår i: Fuel processing technology. - Amsterdam : Elsevier. - 0378-3820 .- 1873-7188. ; 89:12, s. 1324-1329
-
Tidskriftsartikel (refereegranskat)abstract
- In order to study the influence of raw material particle size distribution on the pelletizing process and the physical and thermomechanical characteristics of typical fuel pellets, saw dust of Scots pine was used as raw material for producing pellets in a semi industrial scaled mill (similar to 300 kg h(-1)). The raw materials were screened to a narrow particle size distribution and mixed into four different batches and then pelletized under controlled conditions. Physical pellet characteristics like compression strength, densities, moisture content, moisture absorption and abrasion resistance were determined. In addition, the thermochemical characteristics, i.e. drying and initial pyrolysis, flaming pyrolysis, char combustion and char yield were determined at different experimental conditions by using a laboratory-scaled furnace. The results indicate that the particle size distribution had some effect on current consumption and compression strength but no evident effect on single pellet and bulk density, moisture content, moisture absorption during storage and abrasion resistance. Differences in average total conversion time determined for pellet batches tested under the same combustion conditions was less than 5% and not significant. The results are of practical importance suggesting that grinding of saw dust particle sizes below 8 mm is probably needless when producing softwood pellets. Thus it seem that less energy could be used if only over sized particles are grinded before pelletizing.
|
|
| 10. |
- Bergström, Maria, et al.
(författare)
-
Cholera toxin inhibitors studied with High-performance liquid affinity chromatography: arobust method to evaluate receptor-ligand interactions
- 2009
-
Ingår i: Chemical Biology and Drug Design. - : Wiley. - 1747-0277 .- 1747-0285. ; 73:1, s. 132-141
-
Tidskriftsartikel (refereegranskat)abstract
- Anti-adhesion drugs may be an alternative to antibiotics to control infection of micro-organisms. The well-characterized interaction between cholera toxin and the cellular glycolipid GM1 makes it an attractive model for inhibition studies in general. In this report, we demonstrate a high-performance liquid affinity chromatography approach called weak affinity chromatography to evaluate cholera toxin inhibitors. The cholera toxin B-subunit was covalently coupled to porous silica and a (weak) affinity column was produced. The K(D) values of galactose and meta-nitrophenyl alpha-d-galactoside were determined with weak affinity chromatography to be 52 and 1 mm, respectively, which agree well with IC(50) values previously reported. To increase inhibition potency multivalent inhibitors have been developed and the interaction with multivalent glycopolypeptides was also evaluated. The affinity of these compounds was found to correlate with the galactoside content but K(D) values were not obtained because of the inhomogeneous response and slow off-rate from multivalent interactions. Despite the limitations in obtaining direct K(D) values of the multivalent galactopolypeptides, weak affinity chromatography represents an additional and valuable tool in the evaluation of monovalent as well as multivalent cholera toxin inhibitors. It offers multiple advantages, such as a low sample consumption, high reproducibility and short analysis time, which are often not observed in other methods of analysis.
|
|
