| 1. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
- Baranowska-Rataj, Anna, 1980-, et al.
(författare)
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Parental unemployment and adolescent wellbeing : The moderating role of educational policies
- 2021
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Crossover effects of parental unemployment on wellbeing of children attract growing attention in research on social inequalities. Recent economic crises call for identifying policies that mitigate the adverse effects of unemployment. Building on the theoretical insights from Capability Approach, we examine the relationship between parental unemployment and wellbeing of adolescents across countries with different educational policies. We use multilevel modelling and microdata on economic and subjective wellbeing of household members from the European Union Statistics on Income and Living Conditions (EU-SILC). We combine microdata on 45,992 adolescents in 32 countries with macro-level indicators of educational policies.We find that parental unemployment is associated with lower adolescent wellbeing, but the magnitude of this association varies depending on access to financial support for participation in education. Adolescents who receive educational allowances and who live in countries with broader access to such support are less harmed by parental unemployment.
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| 3. |
- Baranowska-Rataj, Anna, 1980-, et al.
(författare)
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Parental unemployment and adolescents' subjective wellbeing : the moderating role of educational policies
- 2024
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Ingår i: European Sociological Review. - : Oxford University Press. - 0266-7215 .- 1468-2672. ; 40:2, s. 276-292
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Tidskriftsartikel (refereegranskat)abstract
- Crossover effects of parental unemployment on subjective wellbeing of children attract growing attention in research on social inequalities. Recent economic crises call for identifying policies that mitigate the adverse effects of unemployment. Building on the theoretical insights from Capability Approach, we examine the relationship between parental unemployment and subjective wellbeing of adolescents across countries with different educational policies. We use multilevel modelling and data from the European Union Statistics on Income and Living Conditions (EU-SILC). We combine microdata on 45,992 adolescents in 32 countries with macro-level indicators of educational policies. We find that parental unemployment is associated with lower subjective wellbeing among adolescents, but the magnitude of this association varies depending on access to financial support for participation in education. Adolescents who receive educational allowances and who live in countries with broader access to such support are less harmed by parental unemployment.
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| 4. |
- Cheshev, Pavel, et al.
(författare)
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Synthesis and affinity evaluation of a small library of bidentate cholera toxin ligands: towards nonhydrolyzable ganglioside mimics
- 2010
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Ingår i: Chemistry - A European Journal. - : Wiley. - 0947-6539 .- 1521-3765. ; 16:6, s. 1951-1967
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Tidskriftsartikel (refereegranskat)abstract
- A small library of nonhydrolyzable mimics of GM1 ganglioside, featuring galactose and sialic acid its pharmacophoric carbohydrate residues,, was synthesized and tested. All compounds were synthesized from readily available precursors using high-performance reactions, including click chemistry protocols, and avoiding O-glycosidic bonds. Sonic of the most active molecules also feature a point of further derivatization that can be used for conjugation will, polyvalent aglycons. Their affinity towards cholera toxin was assessed by weak affinity chromatography, which allowed a systematic evaluation and selection of the best candidates. Affinity could be enhanced up to one or two orders of magnitude over the affinity of the individual pharmacophoric sugar residues.
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| 5. |
- Garriga, Anna, et al.
(författare)
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The Socioeconomic Gradient of Shared Physical Custody in Two Welfare States : Comparison Between Spain and Sweden
- 2021
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Ingår i: Shared Physical Custody. - Cham : Springer. - 9783030684815 - 9783030684785 - 9783030684792 ; , s. 181-206
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- This study contributes to the emerging literature on the diffusion of SPC across social strata, by comparing two national contexts, Sweden and Spain, with different prevalence rates of SPC and with diverging social and gender policies in the early XXI century. We draw on the 2006 and 2014 comparative cross-sectional data from the Spanish and Swedish Health Behaviour in School-Aged Children (HBSC), to test two competitive hypotheses (diffusion and diverging destinies hypotheses) on the association of parental socioeconomic status, children’s living arrangements in separated families and their relative prevalence in a populaiton. We also examine whether such association is modified by the great increase in SPC in both countries between 2006 and 2014. We present empirical evidence that, independently from the context, SPC arrangements are more frequent among parents with higher socioeconomic status and sole-custody arrangements among other parents; however, social inequality in post-separation arrangements differ in the two countries over time. In Spain, we find evidence in favour of the diffusion hypothesis with increases in the prevalence of SPC going hand in hand with the diffusion of SPC across social strata. By contrast, the Swedish data support the diverging destinies hypothesis with increases in SPC producing no variation in its social stratification over time.
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| 6. |
- Neyer, Gerda, 1952-, et al.
(författare)
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Feminist Perspectives on Motherhood and Reproduction
- 2011
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Ingår i: Historical Social Research / Historische Sozialforschung. - 0172-6404. ; 36:2, s. 162-176
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Tidskriftsartikel (refereegranskat)abstract
- Motherhood and reproduction have been at the core of the feminist discourse about women's rights ever since its onset. For the first and second feminist movements, the right to abortion and the public recognition of motherhood have been main issues in the discourse on reproduction. Since the last two decades of the 20th century, the potentials of assisted reproductive technologies (ART) have opened up new venues of feminist discourse. In this paper we sketch the main feminist lines of argumentation regarding motherhood and reproduction since the 1970s, and we identify specific shifts in their recurrent issues. We argue that an essential contribution of feminism to the understanding of motherhood as a structuring category has been its insistence on the distinction between biological and social motherhood. Feminist discourse shows how ART has further decomposed biological motherhood and has altered the meaning of motherhood and reproduction. Feminist analysis maintains that despite the rhetoric of choice surrounding ART, these technologies have not increased women’s reproductive freedom. The decomposition of biological motherhood, the medical, legal, and commercial development of reproduction, and the change in the social perception of motherhood have rather established new forms of control over female reproduction.
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| 7. |
- Sacchetto, Daniela, et al.
(författare)
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Mammographic density : Comparison of visual assessment with fully automatic calculation on a multivendor dataset
- 2016
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Ingår i: European Radiology. - : Springer. - 0938-7994 .- 1432-1084. ; 26:1, s. 175-183
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Tidskriftsartikel (refereegranskat)abstract
- To compare breast density (BD) assessment provided by an automated BD evaluator (ABDE) with that provided by a panel of experienced breast radiologists, on a multivendor dataset. Twenty-one radiologists assessed 613 screening/diagnostic digital mammograms from nine centers and six different vendors, using the BI-RADS a, b, c, and d density classification. The same mammograms were also evaluated by an ABDE providing the ratio between fibroglandular and total breast area on a continuous scale and, automatically, the BI-RADS score. A panel majority report (PMR) was used as reference standard. Agreement (kappa) and accuracy (proportion of cases correctly classified) were calculated for binary (BI-RADS a-b versus c-d) and 4-class classification. While the agreement of individual radiologists with the PMR ranged from kappa = 0.483 to kappa = 0.885, the ABDE correctly classified 563/613 mammograms (92 %). A substantial agreement for binary classification was found for individual reader pairs (kappa = 0.620, standard deviation [SD] = 0.140), individual versus PMR (kappa = 0.736, SD = 0.117), and individual versus ABDE (kappa = 0.674, SD = 0.095). Agreement between ABDE and PMR was almost perfect (kappa = 0.831). The ABDE showed an almost perfect agreement with a 21-radiologist panel in binary BD classification on a multivendor dataset, earning a chance as a reproducible alternative to visual evaluation. aEuro cent Individual BD assessment differs from PMR with kappa as low as 0.483. aEuro cent An ABDE correctly classified 92 % of mammograms with almost perfect agreement (kappa = 0.831). aEuro cent An ABDE can be a valid alternative to subjective BD assessment.
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| 8. |
- Settersten, Richard A., et al.
(författare)
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Understanding the effects of Covid-19 through a life course lens
- 2020
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Ingår i: Advances in Life Course Research. - : Elsevier BV. - 1040-2608. ; 45
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Tidskriftsartikel (refereegranskat)abstract
- The Covid-19 pandemic is shaking fundamental assumptions about the human life course in societies around the world. In this essay, we draw on our collective expertise to illustrate how a life course perspective can make critical contributions to understanding the pandemic's effects on individuals, families, and populations. We explore the pandemic's implications for the organization and experience of life transitions and trajectories within and across central domains: health, personal control and planning, social relationships and family, education, work and careers, and migration and mobility. We consider both the life course implications of being infected by the Covid-19 virus or attached to someone who has; and being affected by the pandemic's social, economic, cultural, and psychological consequences. It is our goal to offer some programmatic observations on which life course research and policies can build as the pandemic's short- and long-term consequences unfold.
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| 9. |
- Shen, Xinyi, et al.
(författare)
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Chloride-Based Additive Engineering for Efficient and Stable Wide-Bandgap Perovskite Solar Cells
- 2023
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Ingår i: Advanced Materials. - 0935-9648. ; 35:30
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Tidskriftsartikel (refereegranskat)abstract
- Metal halide perovskite based tandem solar cells are promising to achieve power conversion efficiency beyond the theoretical limit of their single-junction counterparts. However, overcoming the significant open-circuit voltage deficit present in wide-bandgap perovskite solar cells remains a major hurdle for realizing efficient and stable perovskite tandem cells. Here, a holistic approach to overcoming challenges in 1.8 eV perovskite solar cells is reported by engineering the perovskite crystallization pathway by means of chloride additives. In conjunction with employing a self-assembled monolayer as the hole-transport layer, an open-circuit voltage of 1.25 V and a power conversion efficiency of 17.0% are achieved. The key role of methylammonium chloride addition is elucidated in facilitating the growth of a chloride-rich intermediate phase that directs crystallization of the desired cubic perovskite phase and induces more effective halide homogenization. The as-formed 1.8 eV perovskite demonstrates suppressed halide segregation and improved optoelectronic properties.
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| 10. |
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The Demography of Europe
- 2013
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Samlingsverk (redaktörskap) (refereegranskat)abstract
- Over the past decades Europe has witnessed fundamental changes of its population dynamics and population structure. Fertility has fallen below replacement level in almost all European countries, while childbearing behavior and family formation have become more diverse. Life expectancy has increased in Western Europe for both females and males, but has been declining for men in some Eastern European countries. Immigration from non-European countries has increased substantially, as has mobility within Europe. These changes pose major challenges to population studies, as conventional theoretical assumptions regarding demographic behavior and demographic development seem unfit to provide convincing explanations of the recent demographic changes.This book, derived from the symposium on “The Demography of Europe” held at the Max Planck Institute for Demographic Research in Rostock, Germany in November 2007 in honor of Professor Jan M. Hoem, brings together leading population researchers in the area of fertility, family, migration, life-expectancy, and mortality. The contributions present key issues of the new demography of Europe and discuss key research advances to understand the continent’s demographic development at the turn of the 21st century.
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