| 1. |
- Jarvius, Jonas, et al.
(author)
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Digital Quantification using Amplified Single-Molecule Detection
- 2006
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In: Nature Methods. - 1548-7091 .- 1548-7105. ; 3:9, s. 725-727
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Journal article (peer-reviewed)abstract
- We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.
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| 2. |
- Bertilsson, Jonas, et al.
(author)
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Discourses on transformational change and paradigm shift in the Green Climate Fund: the divide over financialization and country ownership
- 2021
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In: Environmental Politics. - : Informa UK Limited. - 0964-4016 .- 1743-8934. ; 30:3, s. 423-441
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Journal article (peer-reviewed)abstract
- The Green Climate Fund (GCF) provides climate finance to both adaptation and mitigation projects. Since its establishment in 2010 it has been committed to country ownership and the needs of recipient countries. Interpretations of this commitment are shaped by the GCF’s guiding principles of transformational change and paradigm shift. These principles are used as discursive resources to form the content in project proposals, and to legitimize a top-down financialization of recipient countries, while describing it as country ownership and responsiveness to their needs. This is an example of how climate finance governance becomes a gateway for a deeper financialization of recipient countries.
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| 3. |
- Bertilsson, Jonas, et al.
(author)
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Indigenous peoples and inclusion in the green climate fund
- 2023
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In: Environmental Sociology. - : Routledge. - 2325-1042. ; 9:3, s. 233-242
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Journal article (peer-reviewed)abstract
- In this paper, we explore Indigenous peoples' engagement and inclusion in the Green Climate Fund. We rely on the distinction between simple inclusion and a deeper recognition of Indigenous peoples' contributions, described as epistemic belonging. We analyse how organizational interdependencies, i.e. the exchange and valuation of resources between actors, and how the potential conflicts between contributions from different actors may influence to what degree Indigenous peoples can achieve epistemic belonging. To illustrate this we have analysed the struggles and tensions around the establishment of the Indigenous People Policy (IPP) of the Green Climate Fund (GCF), and the practical use of the IPP in funding proposal discussions and decisions. We conclude that Indigenous peoples' contributions are valued as long as they do not challenge other important GCF interests. Conflicts between contributions from different actors lead to a prioritization of recourses provided by accredited entities that help the GCF to develop, implement and manage climate projects. Hence, Indigenous peoples' contributions become subordinated which provides an obstacle to full epistemic belonging.
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| 4. |
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| 5. |
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| 6. |
- Bertilsson, Jonas
(author)
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The Governance of Global Climate Finance – The Management of Contradictions, Ambiguities and Conflicts in the Green Climate Fund
- 2022
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Doctoral thesis (other academic/artistic)abstract
- Global climate governance struggles with many contentious issues, often made visible in the annual climate meetings arranged by the United Nations Framework Convention on Climate Change (UNFCCC). When these issues are delegated to climate organizations within the UNFCCC system and operationalized into climate practices they are often translated. Climate organizations often take on a technical role, making the contentiousness of climate issues invisible. The thesis investigates one of the major organizations within the UNFCCC, the Green Climate Fund (GCF). The aim of the thesis is to make the contradictions, ambiguities and conflicts around the operationalization of different issues in the GCF visible with the help of immanent critique, and to analyse how the GCF management of these contradictions, ambiguities and conflicts influence the aspects of global climate finance governance issues that become emphasized or subordinated. The thesis consists of four studies examining the operationalization of different issues in the GCF. Study 1 focuses on what factors influence the design of the GCF stakeholder arrangement and how it affects the possibility of different stakeholders to engage actively in the GCF. The GCF makes a clear commitment to support the active engagement of diverse stakeholders. It is argued that the arrangement actually privileges private sector stakeholders. Study 2 examines conflicts around the interpretation of the GCF governing principle transformational change. Some actors in the GCF try to connect transformational change to a financialization of the GCF, while others oppose such development. It is argued that financialization might contradict country ownership, another important principle in the GCF. Study 3 investigates the GCF understanding of climate vulnerability. The analysis shows how the GCF emphasis on dominant logics such as science and market logics reduce the aspects of climate vulnerability that become visible in the GCF, and how the principle of transformational change is implicated in this. The aspects of vulnerability that become visible in the GCF are those that can be managed through calculative logics, while moral and political dimensions become invisible. Study 4 explores the inclusion of indigenous peoples in GCF through an analysis of the development of the GCF Indigenous Peoples Policy. GCF embraces the ‘traditional knowledge’ of indigenous peoples but indigenous peoples find it difficult to introduce a more holistic view of nature, while western science dominates knowledge production. The analysis also shows that the use of this policy in the GCF is limited, despite protests from indigenous peoples’ representatives. Altogether, the studies show that the dominant logics discussed in previous research such as science and market logics play a big role in the GCF. What these dominant logics bring forward are aspects of climate finance issues that are manageable through calculative logics, while perspectives and interests that are not easily compatible with these logics become subordinated – often the political dimensions of climate finance governance. This goes against the GCF portrayal of itself as an inclusive organization that is responsive to a variety of perspectives and interests.
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| 7. |
- Heinrich, Kristina, et al.
(author)
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Molecular Basis and Ecological Relevance of Caulobacter Cell Filamentation in Freshwater Habitats
- 2019
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In: mBio. - : AMER SOC MICROBIOLOGY. - 2161-2129 .- 2150-7511. ; 10:4
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Journal article (peer-reviewed)abstract
- All living cells are characterized by certain cell shapes and sizes. Many bacteria can change these properties depending on the growth conditions. The underlying mechanisms and the ecological relevance of changing cell shape and size remain unclear in most cases. One bacterium that undergoes extensive shape-shifting in response to changing growth conditions is the freshwater bacterium Caulobacter crescentus. When incubated for an extended time in stationary phase, a subpopulation of C. crescentus forms viable filamentous cells with a helical shape. Here, we demonstrated that this stationary-phase-induced filamentation results from downregulation of most critical cell cycle regulators and a consequent block of DNA replication and cell division while cell growth and metabolism continue. Our data indicate that this response is triggered by a combination of three stresses caused by prolonged growth in complex medium, namely, the depletion of phosphate, alkaline pH, and an excess of ammonium. We found that these conditions are experienced in the summer months during algal blooms near the surface in freshwater lakes, a natural habitat of C. crescentus, suggesting that filamentous growth is a common response of C. crescentus to its environment. Finally, we demonstrate that when grown in a biofilm, the filamentous cells can reach beyond the surface of the biofilm and potentially access nutrients or release progeny. Altogether, our work highlights the ability of bacteria to alter their morphology and suggests how this behavior might enable adaptation to changing environments.
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| 8. |
- Kyrkander, Örnborg, et al.
(author)
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Makrofyter i Mälaren 2011
- 2012
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Reports (other academic/artistic)abstract
- Rapporten redovisar inventeringar av makrofyter i Mälaren 2011. Inventering av undervattensväxter är genomförd i tio geografiskt avgränsade delområden i Mälaren, dels för att lokalisera lämpliga miljöövervakningsområden men också för att göra en bedömning av vattenförekomsternas ekologiska status utifrån förekommande vattenväxter. Fem av dessa delområden har till viss del inventerats 2006; Blacken, Asköviken, Gisselfjärden, Granfjärden och Sörfjärden. Metodiken vid inventeringen av Mälaren har i huvudsak följt Naturvårdsverkets undersökningstyp makrofyter i sjöar 2010. Sammanlagt gjordes fynd av 48 arter vattenväxter vid de undersökta delområdena. Södra Björkfärden bedöms ha God ekologis status enligt Naturvårdsverkets bedömningsgrunder. Resterande inventerade områden bedöms ha Måttlig ekologisk status enligt samma bedömningsgrunder. I många delområden är siktdjupet begränsat och man kan anta att vattnet har en hög näringshalt. Detta räcker dock inte för att förklara den generellt låga bedömning flertalet inventerade delområden i Mälaren får. Tyvärr är inte det verktyg som finns till hands för att utvärdera ekologisk status, med avseende på artfynd av makrofyter, helt optimala för sjöar av mer naturligt näringsrik karaktär. Bedömningen för dessa sjöar/delområden blir därmed generellt sämre än vad som möjligen är rimligt. I rapporten görs därför en kompletterande bedömning som helt bygger på rapportförfattarnas egna erfarenheter och synpunkter. Denna bedömning bygger på siktdjupet vid inventerat delområde, maximala djuputbredningen samt fördelningen mellan olika arter. Utifrån denna kompletterande bedömning anses Gripsholmsfjärden samt Brofjärden och Näsfjärden (enligt författarna) ha egenskaper som skulle kunna ge den något bättre bedömning än måttlig ekologisk status. Just nu pågår en utvärdering av bedömningsgrunderna, vilket förhoppningsvis leder till en ny mer rättvisande statusbedömning i framtiden. I denna rapport bygger dock statusklassningen helt på Naturvårdsverkets bedömningsgrunder, även om det möjligen inte är helt lämpliga för en utvärdering av Mälaren. Anledningen till detta är att rapportförfattarna anser att en ”ny bedömning” gjord enligt våra värderingar skulle vara vansklig och resultatet svårt för någon annan att följa upp. Inventeringen 2011 utfördes under augusti månad då månadsmedel för vattenståndet i Mälaren låg på 0,26 möh, samma medelvattenstånd som augusti 2006. Lämpliga lokaler för miljöövervakning är Brofjärden/Näsfjärden, Blacken, Asköviken, Gisselfjärden,Granfjärden, Sörfjärden, Södra Björkfjärden och Gripsholmsfjärden.
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| 9. |
- Meirose, Bernhard, et al.
(author)
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Real-time accelerator diagnostic tools for the max iv storage rings
- 2020
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In: Instruments. - : MDPI AG. - 2410-390X. ; 4:3
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Journal article (peer-reviewed)abstract
- In this paper, beam diagnostic and monitoring tools developed by the MAX IV Operations Group are discussed. In particular, beam position monitoring and accelerator tunes visualization software tools, as well as tools that directly influence the beam quality and stability, are introduced. An availability and downtime monitoring application is also presented.
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| 10. |
- Oldgren, Jonas, 1964-, et al.
(author)
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Early versus delayed non-vitamin k antagonist oral anticoagulant therapy after acute ischemic stroke in atrial fibrillation (timing) : a registry-based randomized controlled noninferiority study
- 2022
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In: Circulation. - : American heart association. - 0009-7322 .- 1524-4539. ; 146:14, s. 1056-1066
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Journal article (peer-reviewed)abstract
- BACKGROUND: There are no evidence-based recommendations on the optimal time point to initiate non-vitamin K antagonist oral anticoagulants (NOACs) after acute ischemic stroke in patients with atrial fibrillation. We aimed to investigate the efficacy and safety of early versus delayed initiation of NOAC in these patients.METHODS: TIMING (Timing of Oral Anticoagulant Therapy in Acute Ischemic Stroke With Atrial Fibrillation) was a registry-based, randomized, noninferiority, open-label, blinded end-point study at 34 stroke units using the Swedish Stroke Register for enrollment and follow-up. Within 72 hours from stroke onset, patients were randomized to early (≤4 days) or delayed (5-10 days) NOAC initiation, with choice of NOAC at the investigators' discretion. The primary outcome was the composite of recurrent ischemic stroke, symptomatic intracerebral hemorrhage, or all-cause mortality at 90 days. The prespecified noninferiority margin was 3%. Secondary outcomes included the individual components of the primary outcome.RESULTS: Between April 2, 2017, and December 30, 2020, 888 patients were randomized to either early (n=450) or delayed (n=438) initiation of NOAC. No patient was lost to 90-day follow-up. Mean age was 78.3 years (SD, 9.9 years); 46.2% were women; 49.1% had previously known atrial fibrillation; and 17.5% prior stroke. The primary outcome occurred in 31 patients (6.89%) assigned to early initiation and in 38 patients (8.68%) assigned to delayed NOAC initiation (absolute risk difference, -1.79% [95% CI, -5.31% to 1.74%]; Pnoninferiority=0.004). Ischemic stroke rates were 3.11% and 4.57% (risk difference, -1.46% [95% CI, -3.98% to 1.07%]) and all-cause mortality rates were 4.67% and 5.71% (risk difference, -1.04% [95% CI, -3.96% to 1.88%]) in the early and delayed groups, respectively. No patient in either group experienced symptomatic intracerebral hemorrhage.CONCLUSIONS: Early initiation was noninferior to delayed start of NOAC after acute ischemic stroke in patients with atrial fibrillation. Numerically lower rates of ischemic stroke and death and the absence of symptomatic intracerebral hemorrhages implied that the early start of NOAC was safe and should be considered for acute secondary stroke prevention in patients eligible for NOAC treatment.
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