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| 2. |
- Ericsson, Henrik, et al.
(författare)
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Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis
- 1995
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Ingår i: Applied and Environmental Microbiology. - : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 61:11, s. 3872-3874
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Tidskriftsartikel (refereegranskat)abstract
- Altogether, 133 strains of Listeria monocytogenes serovar 4b were investigated, A segment of 2,916 bp containing parts of the two genes inlA and inlB in L. monocytogenes was amplified by the PCR technique. The PCR product obtained was cleaved with the restriction enzyme AluI, and the fragments generated were separated by gel electrophoresis, leading to two distinct groups: PCR-restriction enzyme analysis groups I and II, containing 37 and 96 strains, respectively, The PCR-restriction enzyme analysis method described in this paper could be a useful tool for the subtyping of L. monocytogenes serovar 4b strains.
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| 3. |
- Loncarevic, Semir, et al.
(författare)
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Characterization of Listeria strains isolated from soft and semi-soft cheeses
- 1998
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Ingår i: Food microbiology (Print). - : Elsevier. - 0740-0020 .- 1095-9998. ; 15:5, s. 521-525
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Tidskriftsartikel (refereegranskat)abstract
- Two hundred strains ofListeria monoctyogenespreviously isolated from 19 soft and semi-soft cheeses by enrichment were characterized by serotyping and pulsed-field gel electrophoresis (PFGE). Strains of serogroup 1/2 predominated, with 33.5% of all strains belonging to serovar 1/2a, 58.5% to serovar 1/2b and 5% to serovar 1/2c. By using a PFGE method, 16 different clonal types were obtained. All 10 isolates ofL. monocytogenesrecovered from one white mould cheese were serovar 1/2a, but displayed two different PFGE profiles. Another 10L. monocytogenesisolates from white mould cheese recovered after a 48-h enrichment broth procedure revealed two clonal types, only one of which could be detected after 5 additional days of incubation in the same enrichment broth. Cross-contamination in dairy plants and/or retail stores may play an important role in the incidence and diversity ofL. monocytogenesclonal types recovered from soft and semi-soft cheeses. Therefore, it is necessary to characterize several isolates from the same sample when conducting epidemiological investigations.Copyright © 1998 Academic Press. All rights reserved.
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| 4. |
- Parihar, Vishal Singh, et al.
(författare)
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Characterization of human invasive isolates of Listeria monocytogenes in Sweden 1986-2007
- 2008
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Ingår i: Foodborne pathogens and disease. - : Mary Ann Liebert. - 1535-3141 .- 1556-7125. ; 5:6, s. 755-761
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Tidskriftsartikel (refereegranskat)abstract
- Since 1986, 68% of the Listeria monocytogenes isolates from human cases of invasive listeriosis in Sweden are available for retrospective studies. The aim of the present study was to characterize 601 human invasive isolates of L. monocytogenes in Sweden from 1986 to 2007 by using serotyping and pulsed-field gel electrophoresis. Since 1996, serovar 4b was permanently reduced to the second or third most common serovar in human cases in Sweden. During the latter period, 2000-2007, only 13% belonged to serovar 4b and 71% to 1/2a. The dendrogram, based on pulsovars, reveals two clusters with different serovars. Cluster 1 exhibits serovars 4b and 1/2b, whereas cluster 2 consists of serovar 1/2a. Serovar 1/2a seems to be more heterogeneous than serovar 4b.
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| 5. |
- Tham, Wilhelm, 1951-, et al.
(författare)
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Listeria monocytogenes subtypes associated with mortality among fallow deer (Dama dama)
- 1999
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Ingår i: Journal of zoo and wildlife medicine. - 1042-7260 .- 1937-2825. ; 30:4, s. 545-549
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Tidskriftsartikel (refereegranskat)abstract
- Different subtypes of Listeria monocytogenes were isolated from various animal and environmental samples during an episode of increased mortality on a fallow deer (Dama dama) farm. During a 4-wk period, six fallow deer died, including four does, one fawn, and one adult buck. Prior to death, one of the does had exhibited central nervous system signs characteristic of listeriosis. Postmortem examination of the six deer showed no histologic changes typical of listeriosis, although inflammatory changes were present in several organs. Different subtypes of L. monocytogenes were isolated from brain samples from six deer, from fodder and soil from the deer feeding area, and from faces of some healthy animals on the farm. Listeria monocytogenes, which was frequently isolated in the environment of the farm, was considered the probable major cause of mortality in these fallow deer.
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| 6. |
- Tham, Wilhelm, 1951-, et al.
(författare)
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Swedish human cases of Listeriosis, 1958–1999 : Visulized by LEGO Bricks
- 2000
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Ingår i: Nordic PFGE meeting January 27–28, 2000. Collection of abstracts. Department of Food Hygiene, Faculty of Veterinary Medicine, SLU, Uppsala. - Uppsala : Dep of Food Hygiene, Faculty of Veterinary Medicine, SLU. - 9157659621 ; , s. 27-27
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Konferensbidrag (refereegranskat)
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| 7. |
- Unnerstad, Helle, et al.
(författare)
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Division of Listeria monocytogenes serovar 1/2a strains into two groups by PCR and restriction enzyme analysis
- 1999
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Ingår i: Applied and Environmental Microbiology. - Washington DC, USA : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 65:5, s. 2054-2056
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Tidskriftsartikel (refereegranskat)abstract
- Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), and 1,876 bp of the gene inlB, was cleaved with the enzyme AluI, and the fragments generated were separated by gel electrophoresis. By this method two different cleavage patterns were obtained. Seventy of the 100 strains shared one restriction profile, and the remaining 30 strains shared the second one. No relation was found between the types differentiated by PCR-REA and the origins of the strains.
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| 8. |
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