| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Voight, Benjamin F, et al.
(författare)
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Plasma HDL cholesterol and risk of myocardial infarction : a mendelian randomisation study
- 2012
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Ingår i: The Lancet. - 0140-6736 .- 1474-547X. ; 380:9841, s. 572-580
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: High plasma HDL cholesterol is associated with reduced risk of myocardial infarction, but whether this association is causal is unclear. Exploiting the fact that genotypes are randomly assigned at meiosis, are independent of non-genetic confounding, and are unmodified by disease processes, mendelian randomisation can be used to test the hypothesis that the association of a plasma biomarker with disease is causal.METHODS: We performed two mendelian randomisation analyses. First, we used as an instrument a single nucleotide polymorphism (SNP) in the endothelial lipase gene (LIPG Asn396Ser) and tested this SNP in 20 studies (20,913 myocardial infarction cases, 95,407 controls). Second, we used as an instrument a genetic score consisting of 14 common SNPs that exclusively associate with HDL cholesterol and tested this score in up to 12,482 cases of myocardial infarction and 41,331 controls. As a positive control, we also tested a genetic score of 13 common SNPs exclusively associated with LDL cholesterol.FINDINGS: Carriers of the LIPG 396Ser allele (2·6% frequency) had higher HDL cholesterol (0·14 mmol/L higher, p=8×10(-13)) but similar levels of other lipid and non-lipid risk factors for myocardial infarction compared with non-carriers. This difference in HDL cholesterol is expected to decrease risk of myocardial infarction by 13% (odds ratio [OR] 0·87, 95% CI 0·84-0·91). However, we noted that the 396Ser allele was not associated with risk of myocardial infarction (OR 0·99, 95% CI 0·88-1·11, p=0·85). From observational epidemiology, an increase of 1 SD in HDL cholesterol was associated with reduced risk of myocardial infarction (OR 0·62, 95% CI 0·58-0·66). However, a 1 SD increase in HDL cholesterol due to genetic score was not associated with risk of myocardial infarction (OR 0·93, 95% CI 0·68-1·26, p=0·63). For LDL cholesterol, the estimate from observational epidemiology (a 1 SD increase in LDL cholesterol associated with OR 1·54, 95% CI 1·45-1·63) was concordant with that from genetic score (OR 2·13, 95% CI 1·69-2·69, p=2×10(-10)).INTERPRETATION: Some genetic mechanisms that raise plasma HDL cholesterol do not seem to lower risk of myocardial infarction. These data challenge the concept that raising of plasma HDL cholesterol will uniformly translate into reductions in risk of myocardial infarction.
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| 3. |
- Abdallah, J., et al.
(författare)
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A determination of the centre-of-mass energy at LEP2 using radiative two-fermion events
- 2006
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Ingår i: European Physical Journal C. Particles and Fields. - : Springer Science and Business Media LLC. - 1434-6044 .- 1434-6052. ; 46:2, s. 295-305
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Tidskriftsartikel (refereegranskat)abstract
- Using e(+)e(-) -> mu(+)mu(-)(gamma) and e(+)e(-) -> q (q) over bar(gamma) events radiative to the Z pole, DELPHI has determined the centre-of-mass energy, root s, using energy and momentum constraint methods. The results are expressed as deviations from the nominal LEP centre-of-mass energy, measured using other techniques. The results are found to be compatible with the LEP Energy Working Group estimates for a combination of the 1997 to 2000 data sets.
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| 4. |
- Abdallah, J, et al.
(författare)
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A measurement of the branching fractions of the b-quark into charged and neutral b-hadrons
- 2003
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Ingår i: Physics Letters. Section B: Nuclear, Elementary Particle and High-Energy Physics. - : Elsevier BV. - 0370-2693. ; 576:1-2, s. 29-42
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Tidskriftsartikel (refereegranskat)abstract
- The production fractions of charged and neutral b-hadrons in b-quark events from Z(0) decays have been measured with the DELPHI detector at LEP. An algorithm has been developed, based on a neural network, to estimate the charge of the weakly-decaying b-hadron by distinguishing its decay products from particles produced at the primary vertex. From the data taken in the years 1994 and 1995, the fraction of (b) over bar -quarks fragmenting into positively charged weakly-decaying b-hadrons has been measured to be: f(+) = (42.09+/-0.82(stat)+/-0.89(syst))%. Subtracting the rates for charged Xi(b)(+) and Omega(b)(+) baryons gives the production fraction of B+ mesons: f(Bu) = (40.99+/-0.82(stat)+/-1.11 (syst)) %.
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| 5. |
- Abdallah, J., et al.
(författare)
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A measurement of the tau hadronic branching ratios
- 2006
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Ingår i: European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6044 .- 1434-6052. ; 46:1, s. 1-26
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Tidskriftsartikel (refereegranskat)abstract
- The exclusive and semi-exclusive branching ratios of the tau lepton hadronic decay modes (h(-)upsilon(tau), h(-)pi(0)upsilon(tau), h(-)pi(0)pi(0)upsilon(tau), h(-) >= 2 pi(0)nu(tau), 2h(-)h(+)upsilon(tau), 2h(-)h(+)>= 2 pi(0)upsilon(tau), 3h(-)2h(+)upsilon(tau) and 3h(-)2h(+) >= 1 pi(0)upsilon(tau)) were measured with data from the DELPHI detector at LEP.
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| 6. |
- Abdallah, J, et al.
(författare)
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A precise measurement of the B+, B-0 and mean b-hadron lifetime with the DELPHI detector at LEP I
- 2004
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Ingår i: European Physical Journal C. Particles and Fields. - : Springer Science and Business Media LLC. - 1434-6044. ; 33:3, s. 307-324
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Tidskriftsartikel (refereegranskat)abstract
- Final results from the DELPHI Collaboration on the lifetime of B+ and B-0 mesons and the mean b-hadron lifetime, are presented using the data collected at the Z(0) peak in 1994 and 1995. Elaborate, inclusive, secondary vertexing methods have been employed to ensure a b-hadron reconstruction with good efficiency. To separate samples of B+ and B-0 mesons, high performance neural network techniques are used that achieve very high purity signals. The results obtained are: tau(B+) = 1.624+/-0.014 (stat)+/-0.018 (syst) ps tau(B)0 = 1.531+/-0.021 (stat)+/-0.031 (syst) ps tau(B+)/tau(B)0 = 1.060+/-0.021 (stat)+/-0.024 (syst) and for the average b-hadron lifetime: taub/tau(B)0 = 1.570+/-0.005 (stat)+/-0.008 (syst) ps.
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| 7. |
- Abdallah, J, et al.
(författare)
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A precise measurement of the tau lifetime
- 2004
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Ingår i: European Physical Journal C. Particles and Fields. - : Springer Science and Business Media LLC. - 1434-6044. ; 36:3, s. 283-296
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Tidskriftsartikel (refereegranskat)abstract
- The tau lepton lifetime has been measured with the e(+) e(-) --> tau(+) tau(-) events collected by the DELPHI detector at LEP in the years 1991 - 1995. Three different methods have been exploited, using both one-prong and three-prong tau decay channels. Two measurements have been made using events in which both taus decay to a single charged particle. Combining these measurements gave tau(tau) ( 1 prong) = 291.8 +/- 2.3(stat) +/- 1.5(sys) fs. A third measurement using taus which decayed to three charged particles yielded tau(tau) (3 prong) = 288.6 +/- 2.4(stat) +/- 1.3(sys) fs. These were combined with previous DELPHI results to measure the tau lifetime, using the full LEP1 data sample, to be tau(tau) = 290.9 +/- 1.4(stat) +/- 1.0(sys) fs.
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| 8. |
- Abdallah, J., et al.
(författare)
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A study of b(b)over-bar production in e(+)e(-) collisions at root s=130-207 GeV
- 2009
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Ingår i: European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6044 .- 1434-6052. ; 60:1, s. 1-15
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Tidskriftsartikel (refereegranskat)abstract
- Measurements are presented of R-b, the ratio of the b (b) over bar cross-section to the q (q) over bar cross-section in e(+)e(-) collisions, and the forward-backward asymmetry A(FB)(b) at twelve energy points in the range root s = 130-207 GeV. These results are found to be consistent with the Standard Model expectations. The measurements are used to set limits on new physics scenarios involving contact interactions.
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| 9. |
- Abdallah, J., et al.
(författare)
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A study of the b-quark fragmentation function with the DELPHI detector at LEP I and an averaged distribution obtained at the Z Pole
- 2011
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Ingår i: European Physical Journal C. - : Springer Science and Business Media LLC. - 1434-6044 .- 1434-6052. ; 71:2, s. 1557-
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Tidskriftsartikel (refereegranskat)abstract
- The nature of b-quark jet hadronisation has been investigated using data taken at the Z peak by the DELPHI detector at LEP. Two complementary methods are used to reconstruct the energy of weakly decaying b-hadrons, E-B(weak). The average value of x(B)(weak) = E-B(weak)/E-beam is measured to be 0.699 +/- 0.011. The resulting x(B)(weak) distribution is then analysed in the framework of two choices for the perturbative contribution (parton shower and Next to Leading Log QCD calculation) in order to extract measurements of the non-perturbative contribution to be used in studies of b-hadron production in other experimental environments than LEP. In the parton shower framework, data favour the Lund model ansatz and corresponding values of its parameters have been determined within PYTHIA 6.156 from DELPHI data: a = 1.84(-0.21)(+0.23) and b = 0.642(-0.063)(+0.073) GeV-2, with a correlation factor rho = 92.2%. Combining the data on the b-quark fragmentation distributions with those obtained at the Z peak by ALEPH, OPAL and SLD, the average value of x(B)(weak) is found to be 0.7092 +/- 0.0025 and the non-perturbative fragmentation component is extracted. Using the combined distribution, a better determination of the Lund parameters is also obtained: a = 1.48(-0.10)(+0.11) and b = 0.509(-0.023)(+0.024) GeV-2, with a correlation factor rho = 92.6%.
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| 10. |
- Abdallah, J, et al.
(författare)
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A study of the energy evolution of event shape distributions and their means with the DELPHI detector at LEP
- 2003
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Ingår i: European Physical Journal C. Particles and Fields. - : Springer Science and Business Media LLC. - 1434-6044. ; 29:3, s. 285-312
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Tidskriftsartikel (refereegranskat)abstract
- Infrared and collinear safe event shape distributions and their mean values are determined in e(+)e(-) collisions at centre-of-mass energies between 45 and 202GeV. A phenomenological analysis based on power correction models including hadron mass effects for both differential distributions and mean values is presented. Using power corrections, alpha(s) is extracted from the mean values and shapes. In an alternative approach, renormalisation group invariance (RGI) is used as an explicit constraint, leading to a consistent description of mean values without the need for sizeable power corrections. The QCD beta-function is precisely measured using this approach. From the DELPHI data on Thrust, including data from low energy experiments, one finds beta(0) = 7.86 +/- 0.32 for the one loop coefficient of the beta-function or, assuming QCD, n(f) = 4.75 +/- 0.44 for the number of active flavours. These values agree well with the QCD expectation of beta(0) = 7.67 and n(f) 5. A direct measurement of the full logarithmic energy slope excludes light gluinos with a mass below 5GeV.
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