| 1. |
- Ge, Huifang, et al.
(författare)
-
Egg white peptides ameliorate dextran sulfate sodium-induced acute colitis symptoms by inhibiting the production of pro-inflammatory cytokines and modulation of gut microbiota composition
- 2021
-
Ingår i: Food Chemistry. - : Elsevier. - 0308-8146 .- 1873-7072. ; 360
-
Tidskriftsartikel (refereegranskat)abstract
- Egg white peptides (EWPs) can be effectively used to alleviate and treat inflammatory diseases due to their anti-oxidation, anti-inflammation, and microbiota regulation capabilities. A dextran sodium sulfate (DSS)-induced colitis model was used to clarify the regulatory effects of EWPs on colitis. Forty-three peptide sequences were identified from EWPs using LC-MS/MS. The results demonstrated that EWPs decreased the levels of pro-inflammatory cytokines and the extent of crypt damage in a dose-dependent manner. 16S rRNA gene sequencing results indicated that 200 mg/kg EWPs significantly increased the relative abundance of beneficial bacteria Lactobacillus and Candidatus Saccharimonas, and reduced the relative abundance of pathogenic bacteria Ruminiclostridium and Akkermansia. In addition, the degree of correlation between pro-inflammatory cytokines and microbiota was as follows: interleukin (IL)-1 beta > IL-8 > IL-6 > tumor necrosis factor-alpha To summarize, EWPs contributed to the alleviation of colitis symptoms and the intestinal injury through anti-inflammatory effects, repair of intestinal mucosa, and modulation of gut microbiota.
|
|
| 2. |
- Hedi, Wen, et al.
(författare)
-
γ-Cyclodextrin-BSA for nano-encapsulation of hydrophobic substance
- 2021
-
Ingår i: Food Bioscience. - : Elsevier. - 2212-4292 .- 2212-4306. ; 41
-
Tidskriftsartikel (refereegranskat)abstract
- Self-aggregation and the hemolytic effect limit the application of gamma-cyclodextrin (gamma-CD) in bioactive molecular delivery systems. In this study, gamma-CD was modified by grafting onto bovine serum albumin protein (BSA), with epichlorohydrin (ECH) acting as the cross-linking agent. The effects of BSA concentration, reaction temperature, pH and time on the grafting rate were studied, and the gamma-CD-BSA complex with a grafting rate of 99.5 +/- 0.1% +/- 0.06)% was achieved. The complex was confirmed using H-1 NMR and FT-IR spectra. Compared with gamma-CD, the hemolytic effect and self-aggregation of gamma-CD-BSA were significantly reduced, and the encapsulation efficiency of curcumin was increased by 10.8%. The results of scanning electron microscopy showed that both gamma-CD and gamma-CDBSA nanoparticles were formed and the structure of the gamma-CD-BSA complex was more uniform. The pH stability and salt stability of gamma-CD-BSA were higher than gamma-CD. The release rate of gamma-CD-BSA was 15.2 +/- 0.2% after 2 h at pH 1.2, and 57 +/- 1% after 4 h at pH 7.2. The gamma-CD-BSA nanoparticles could protect curcumin in acidic environments and release it in neutral environments. The results suggested a promising wall material for delivery of hydrophobic substances.
|
|
