| 1. |
- Ahuja-Jensen, Poonam, et al.
(författare)
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Low glutathione peroxidase in rdl mouse retina increases oxidative stress and proteases
- 2007
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Ingår i: NeuroReport. - 1473-558X. ; 18:8, s. 797-801
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Tidskriftsartikel (refereegranskat)abstract
- Malondialdehyde, reduced glutathione, glutathione peroxidase, glutathione reductase and cysteine protease cathepsins at postnatal (PN) days 2, 7, 14, 21 and 28 in controls (wt) and the retinal degeneration 1 (rd1) mouse model for retinitis pigmentosa retinas were measured to determine oxidative stress. In PN28 wt and PN2 rd1 retinas, elevated malondialdehyde and low glutathione peroxidase activity indicate higher oxidative load, despite higher reduced glutathione in PN2 rd1 retinas. This is due to physiological exposure to light and retinal vascular/neural restructuring, respectively. Compared with wt retinas, relatively high malondialdehyde at PN2 and cathepsin levels at PN14, 21 and 28 in rd1 retinas indicate that cells of the residual inner retina also contribute to the oxidative stress and retinal degeneration.
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| 2. |
- Miranda, Maria, et al.
(författare)
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Antioxidants rescue photoreceptors in rd1 mice: Relationship with thiol metabolism
- 2010
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Ingår i: Free Radical Biology & Medicine. - : Elsevier BV. - 0891-5849. ; 48:2, s. 216-222
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Tidskriftsartikel (refereegranskat)abstract
- We have previously shown that the use of a combination of antioxidants delayed the degeneration process in rd1 mouse retina. In an effort to understand the mechanism of action of these substances (zeaxanthin, lutein, alpha-lipoic acid, glutathione, and Lycium barbarum extract) the changes in the levels of several proteins and oxidative stress markers in the rd1 retina have been studied. The treatment increased glutathione peroxidase activity and glutathione levels and decreased cystine concentrations in rd1 retinas. Considering all the results obtained from treated and untreated animals, a high correlation was present between glutathione concentration and glutathione peroxidase activity, and there was a negative correlation between glutathione retinal concentration and number of TUNEL-positive cells. No difference was observed between the numbers of nNOS- and NADPH-diaphorase-positive cells in treated and untreated rd1 mice. Thiol contents and thiol-dependent peroxide metabolism seem to be directly related to the survival of photoreceptors in rd1 mouse retina. (C) 2009 Elsevier Inc. All rights reserved.
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