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Sökning: WFRF:(Boyer Kristy Elizabeth)

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Matviienko, Andrii, et al. (författare)
  • Learning from Cycling: Discovering Lessons Learned from CyclingHCI
  • 2024
  • Ingår i: CHI 2024 - Extended Abstracts of the 2024 CHI Conference on Human Factors in Computing Sytems. - : Association for Computing Machinery (ACM).
  • Konferensbidrag (refereegranskat)abstract
    • Cycling plays an essential role in sustainable mobility, health, and socializing. This workshop aims to collect and discuss the lessons learned from Cycling Human-Computer Interaction (CyclingHCI). For this, we will gather researchers and experts in the field to discuss what we learned from designing, building, and evaluating CyclingHCI systems. We will start the workshop with three lessons learned from CyclingHCI defined by the organizers and their experience in the field, which include (1) a lack of theories, tools, and perspectives, (2) knowledge about designing for safety and inclusive cycling, and (3) evaluation methods and environments. Taken together, with this work, we aim to promote interactive technology to get more people cycling, profiting from the many associated benefits.
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