| 1. |
- Bräunig, Sandro
(författare)
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Human Bone Marrow Microenvironment in Health and Disease
- 2023
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Hematopoietic stem cells (HSCs) are safeguarded from various threats such as stress, injury, or radiationwithin specialized microenvironments or niches within the bone marrow. In this thesis, we investigatedvarious facets of the bone marrow (BM) microenvironment and its critical role in hematopoiesis andrelated disorders.Initially, we elucidated the role of the Early Growth Response 1 (EGR1) gene in bone marrowmesenchymal stem cells (MSCs). EGR1 expression was found to be significantly elevated in specific BMMSC populations, particularly lin-CD45-CD271+ CD140a- BM MSCs, exerting a pivotal role inhematopoietic stroma support. This support function was mediated through both cell-cell interactions andsoluble factors, where EGR1-overexpressing BM MSCs exhibited enhanced secretion of chemokineligand 28 and increased expression of vascular cell adhesion molecule 1, crucial for hematopoiesissupport. EGR1 also played a dual role in BM MSC proliferation regulation. Understanding thesemechanisms can improve hematopoietic stem cell transplantation and regenerative medicine.Further, employing single-cell RNA sequencing, we provided a comprehensive analysis of the cellularcomposition of human BM stroma, revealing diverse cell populations and stromal progenitors with varyingdifferentiation capacities. We identified and characterized multipotent stromal stem cells (MSSCs), highlyadipocytic gene-expressing progenitors (HAGEPs), pre-osteoblasts, and other stromal clusters, offeringvaluable insights into BM stromal heterogeneity and its structural organization. We employed in silicocluster interaction analysis and found that the different stromal populations are predicted to interact andsupport HSCs differentially in different niches.Next, we introduced a meticulous methodology utilizing multicolor immunofluorescence staining and 3Danalysis to investigate human BM architecture. We illustrated the potential of sequential staining,emphasizing specific structural changes associated with myeloproliferative neoplasms (MPNs) and theircorrelation with CD271 expression.Finally, we investigated human BM architecture and cytokine expression patterns in patients with AcuteLymphoblastic Leukemia (ALL) and Primary Myelofib(PMF) with elaborate immunofluorescence andmRNA-based staining methods and compared them to healthy controls. We revealed significantalterations, including differential numbers of megakaryocytes, differences in cellularity, alteredmesenchymal stem cell density, and distinct cytokine expressions offering critical insights into diseasepathogenesis and progression.Collectively, these findings illuminate various facets of the BM microenvironment, offering valuableinsights into its critical role in hematopoiesis, stromal heterogeneity, and disease pathology, paving theway for potential therapeutic advancements in regenerative medicine and hematological disorders.
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| 2. |
- Bräunig, Sandro, et al.
(författare)
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Three-dimensional spatial mapping of the human hematopoietic microenvironment in healthy and diseased bone marrow
- 2023
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Ingår i: Cytometry Part A. - 1552-4922. ; 103:10, s. 763-776
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Tidskriftsartikel (refereegranskat)abstract
- The bone marrow hematopoietic microenvironment (HME) plays a pivotal role in regulating normal and diseased hematopoiesis. However, the spatial organization of the human HME has not been thoroughly investigated yet. Therefore, we developed a three-dimensional (3D) immunofluorescence model to analyze changes in the cellular architecture in control and diseased bone marrows (BMs). BM biopsies from patients with myeloproliferative neoplasms (MPNs) were stained sequentially for CD31, CD34, CD45, and CD271 with repetitive bleaching steps to realize five color images with DAPI as a nuclear stain. Hematopoietically normal age-matched BM biopsies served as controls. Twelve subsequent slides per sample were stacked to create three-dimensional bone marrow reconstructions with the imaging program Arivis Visions 4D. Iso-surfaces for niche cells and structures were created and exported as mesh objects for spatial distribution analysis in the 3D creation suite Blender. We recapitulated the bone marrow architecture using this approach and produced comprehensive 3D models of endosteal and perivascular BM niches. MPN bone marrows displayed apparent differences compared to the controls, especially concerning CD271 staining density, megakaryocyte (MK) morphology, and distribution. Furthermore, measurements of the spatial relationships of MKs and hematopoietic stem and progenitor cells with vessels and bone structures in their corresponding niche environments revealed the most pronounced differences in the vascular nice in polycythemia vera. Taken together, using a repetitive staining and bleaching approach allowed us to establish a 5-color analysis of human BM biopsies, which is difficult to achieve with conventional staining approaches. Based on this, we generated 3D BM models which recapitulated key pathological features and, importantly, allowed us to define the spatial relationships between different bone marrow cell types. We, therefore, believe that our method can provide new and valuable insights into bone marrow cellular interaction research.
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| 3. |
- Li, Hongzhe, et al.
(författare)
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Early growth response 1 regulates hematopoietic support and proliferation in human primary bone marrow stromal cells
- 2020
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Ingår i: Haematologica. - : Ferrata Storti Foundation (Haematologica). - 1592-8721 .- 0390-6078. ; 105:5, s. 1206-1215
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Tidskriftsartikel (refereegranskat)abstract
- Human bone marrow stromal cells are key elements of the hematopoietic environment and they play a central role in bone and bone marrow physiology. However, how key stromal cell functions are regulated is largely unknown. We analyzed the role of the immediate early response transcription factor EGR1 as key stromal cell regulator and found that EGR1 was highly expressed in prospectively-isolated primary bone marrow stromal cells, downregulated upon culture, and low in non-colony-forming CD45neg stromal cells. Furthermore, EGR1 expression was lower in proliferative regenerating adult and fetal primary cells compared to adult steady-state bone marrow stromal cells. Overexpression of EGR1 in stromal cells induced potent hematopoietic stroma support as indicated by an increased production of transplantable CD34+CD90+ hematopoietic stem cells in expansion co-cultures. The improvement of bone marrow stroma support function was mediated by increased expression of hematopoietic supporting genes, such as VCAM1 and CCL28. Furthermore, EGR1 overexpression markedly decreased stromal cell proliferation whereas EGR1 knockdown caused the opposite effects. These findings thus show that EGR1 is a key stromal transcription factor with a dual role in regulating proliferation and hematopoietic stroma support function that is controlling a genetic program to coordinate the specific functions of bone marrow stromal cells in their different biological contexts.
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| 4. |
- Li, Hongzhe, et al.
(författare)
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Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing
- 2023
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Ingår i: eLife. - : eLife Sciences Publications, Ltd. - 2050-084X. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Hematopoiesis is regulated by the bone marrow (BM) stroma. However, cellular identities and functions of the different BM stromal elements in humans remain poorly defined. Based on single-cell RNA sequencing (scRNAseq), we systematically characterized the human non-hematopoietic BM stromal compartment and we investigated stromal cell regulation principles based on the RNA velocity analysis using scVelo and studied the interactions between the human BM stromal cells and hematopoietic cells based on ligand-receptor (LR) expression using CellPhoneDB. scRNAseq led to the identification of six transcriptionally and functionally distinct stromal cell populations. Stromal cell differentiation hierarchy was recapitulated based on RNA velocity analysis and in vitro proliferation capacities and differentiation potentials. Potential key factors that might govern the transition from stem and progenitor cells to fate-committed cells were identified. In situ localization analysis demonstrated that different stromal cells were localized in different niches in the bone marrow. In silico cell-cell communication analysis further predicted that different stromal cell types might regulate hematopoiesis through distinct mechanisms. These findings provide the basis for a comprehensive understanding of the cellular complexity of the human BM microenvironment and the intricate stroma-hematopoiesis crosstalk mechanisms, thus refining our current view on human hematopoietic niche organization.
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