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- Hänsel Petersson, Gunnel, et al.
(författare)
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Caries risk assessment : a comparison between the computer program ‘Cariogram’, dental students and dental instructors
- 1998
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Ingår i: European journal of dental education. - : Wiley. - 1396-5883 .- 1600-0579. ; 2:4, s. 184-190
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Tidskriftsartikel (refereegranskat)abstract
- An interactive educational PC-program for caries risk evaluation, the ‘Cariogram’, was developed. It illustrates the interaction between caries related factors and expresses the caries risk graphically. It also expresses a weighted impact of different aetiological factors on the risk. The aim of this paper was to use the Cariogram program on a set of patients, and to compare the outcome of its risk evaluation with those made by dental students and faculty teachers. A questionnaire was given to the participants, containing descriptions of 5 patients with detailed information on 9 factors generally associated with caries. The participants were asked to rank the patients according to their ‘chance to avoid dental caries’ during the coming year. The results were compared with the assessments obtained from the Cariogram and the similarity calculated by group average cluster analysis. Results: 86% of the respondents ranked the patients for caries risk either identical or with only 1 deviation compared to the program. No difference was seen between students and teachers. The cluster analysis identified 5 distinct patterns of ranking the patients with a maximum distance of 0.6 compared to the program. It was concluded that the ‘opinion’ on caries risk of the Cariogram was in agreement with that of the majority of the responders. In addition, the use of the program induced discussions about the relative impact of caries aetiological factors, and it is believed that the Cariogram can serve as one further option in the teaching of caries risk.
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| 2. |
- Hänsel Petersson, G, et al.
(författare)
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Comparing caries risk factors and caries risk profiles in children and elderly.
- 2004
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Ingår i: Swedish dental journal. ; 28:3, s. 119-128
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Tidskriftsartikel (refereegranskat)abstract
- The aims of this study were to compare the caries risk profiles of children and elderly, the actual annual caries increment and the impact of some selected caries related factors. Another aim was to find out if there were gender differences among the participants. The risk profiles were created by a computerised risk assessment program, the Cariogram, which evaluates data and presents the weighted and summarized result as one figure, illustrating the ‘percent chance of avoiding caries’ in the future. Methods: The Cariogram was earlier evaluated in two longitudinal studies for its capacity to assess caries risk. One study comprised about 400 children, 10-11 years of age and the other study included about 150 elderly (age 55, 65 and 75). At baseline, information on past caries experience, diet, oral hygiene and use of fluoride was obtained. Saliva analyses included mutans streptococci and lactobacilli, buffering capacity and secretion rate. The caries risk was assessed and the participants were divided into five groups according to the calculated Cariogram risk profiles. After two and five years, respectively, caries was re-evaluated and the incidence was compared with the predictions. Results: The Cariogram risk predictions were statistically in agreement with the actual caries increment. Fifty percent of the children, but only two percent of the elderly appeared in the lowest caries risk group. Of the elderly, 26.4% belonged to the highest caries risk group versus 3.1% of the children. The median value ‘chance of avoiding caries’ was 44% for the elderly and 80% for the children. The main Cariogram sectors contributing to the observed higher caries risk among elderly was the bacterial components in combination with higher susceptibility. Individual factors contributing significantly to the higher risk profiles for the adults compared to the children were higher plaque scores, higher counts of mutans streptococci and lower buffering capacity. Conclusion: comparing the risk profiles of the children and the elderly showed that the elderly were at a higher risk developing caries lesions. Overall one may say that the risk for caries, as assessed by the Cariogram, was twice as high for the elderly.
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| 3. |
- Jonsson, D, et al.
(författare)
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Functional significance of female sex hormone receptors in the periodontium (Dublin)
- 2006
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Konferensbidrag (refereegranskat)abstract
- Objectives: Several studies have addressed the association between changes in estrogen and progesterone levels and changes in parameters of periodontitis. The purpose of this project is to investigate the mechanisms by which estrogen influence structure and function of the periodontal ligament by affecting the properties of periodontal ligament cells (PDL cells). Methods: PDL cells were obtained from teeth extracted for orthodontic reasons. The cells were cultured from periodontal tissue explants and used in passages 3-5. Estrogen (ER) expression was investigated by immunocytochemistry. Subcellular distribution of ERβ was determined using mitotracker. Expression of mitochondrial proteins was done using Western blotting. DNA and collagen synthesis was measured using incorporation of radioactive isotopes. Cytokine expression was investigated using ELISA. Results: ERβ but not ERα immunoreactivity was observed in the PDL cells. Preliminary results show that ERβ is distributed not only in the nucleus but also in the mitochondria. To study the effect of mitochondrial ERβ we will investigate synthesis of mitochondrial proteins. Estrogen increased DNA synthesis in human breast cancer cells but had no effect on PDL cell DNA or collagen synthesis. Conclusion: Human PDL cells express ERβ suggesting that estrogen affects PDL cellular function via this ER subtype. Estrogen has no effect on DNA and collagen synthesis, showing that estrogen has no beneficial effect on the periodontium via this mechanism. Instead estrogen via ERβ acts on the periodontium via another still unknown mechanism, perhaps via mitochondrial function or regulatory effect on cytokines.
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| 4. |
- Jonsson, D, et al.
(författare)
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Functional significance of female sex hormone receptors in the periodontium (Madrid)
- 2006
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Konferensbidrag (refereegranskat)abstract
- Department of Experimental Medical Sciences, Lund University, Sweden. Department of Periodontology, Faculty of Odontology, Malmö University, Sweden. Introduction: Several studies have addressed the association between changes in estrogen and progesterone levels and changes in parameters of periodontitis. The purpose of this project is to investigate the mechanisms by which estrogen influence structure and function of the periodontal ligament by affecting the properties of periodontal ligament cells (PDL cells). Materials and methods: PDL cells were obtained from teeth extracted for orthodontic reasons. The cells were cultured from periodontal tissue explants and used in passages 3-5. Estrogen (ER) and progesterone receptor (PR) expression was investigated by immunocytochemistry. Subcellular distribution of ERβ was determined using mitotracker, immunogoldlabeling and electronmicroscopy. DNA and collagen synthesis was measured using incorporation of radioactive isotopes. Results: ERβ but not ERα or PR immunoreactivity was observed in the PDL cells. Preliminary results show that ERβ is distributed not only in the nucleus but also in the mitochondria and cytosol. Estrogen increased DNA synthesis in human breast cancer cells but had no effect on PDL cell DNA or collagen synthesis. Conclusions: Human PDL cells express ERβ suggesting that estrogen affects PDL cellular function via this ER subtype. Estrogen has no effect on DNA and collagen synthesis, showing that estrogen has no beneficial effect on the periodontium via this mechanism. Instead estrogen via ERβ acts on the periodontium via another still unknown mechanism.
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