| 1. |
|
|
| 2. |
- Berg, K, et al.
(författare)
-
Iodinated radiographic contrast media possess antioxidant properties in vitro
- 2005
-
Ingår i: Acta Radiologica. - : SAGE Publications. - 0284-1851 .- 1600-0455. ; 46:8, s. 815-822
-
Tidskriftsartikel (refereegranskat)abstract
- Purpose: To study potential properties of iodinated radiographic contrast media (IRCM) for intravascular use in in vitro free radical generating reactions. Material and Methods: Superoxide (center dot O-2(-)) and hydroxyl ((OH)-O-center dot) radicals were generated in xanthine oxidase and Fenton reactions. center dot O-2(-) was assayed by the nitroblue tetrazolium (NBT) method, whereas (OH)-O-center dot was assayed by an aromatic hydroxylation (2-hydroxybenzoic acid) method. Total antioxidant status (TAS) of test substances was determined by a colorimetric assay. Finally, acetyl-cholinesterase (AChE) activity was measured in the absence and presence of IRCM. Results: High concentrations (greater than50 mM) of IRCM inhibited center dot O-2(-) production, ionic more than non-ionic IRCM. Medium concentrations (25-50 mM) of IRCM reduced (OH)-O-center dot production, and both types of IRCM were equally potent. Low concentrations (less than25 mM) of non-ionic IRCM displayed higher antioxidant capacity than their ionic counterparts when tested in the TAS assay. Visipaque 320 (iodixanol) was found to have the highest TAS value, followed by Omnipaque 350(R) (iohexol), Hexabrix 320(R) (ioxaglate), and Urografin 370(R) (diatrizoate). Conclusion: IRCM have in vitro antioxidant properties in concentrations relevant for their clinical application. These properties may therefore be of potential importance when evaluating IRCM effects in vivo, particularly those concerning cardiovascular and renal function.
|
|
| 3. |
|
|
| 4. |
- Karlsson, J, et al.
(författare)
-
Cardioprotective effects of the MR contrast agent MnDPDP and its metabolite MnPLED upon reperfusion of the ischemic porcine myocardium
- 2001
-
Ingår i: Acta Radiologica. - 0284-1851 .- 1600-0455. ; 42:6, s. 540-547
-
Tidskriftsartikel (refereegranskat)abstract
- Purpose: To evaluate whether manganese dipyridoxyl diphosphate (MnDPDP) or its metabolite manganese dipyridoxyl ethyldiamine (MnPLED) reduces post-ischemic myocardial injury. Material and Methods: Left anterior descending artery (LAD) in anesthetized pigs was occluded (30 min) followed by reperfusion (120 min) during hemodynamic monitoring and infarct assessment. Three ╡mol/kg MnDPDP, 1 ╡mol/kg MnPLED (or a mixture of both) or saline was injected i.v. 10 min before reperfusion followed by infusion of either 3 ╡mol/kg/h MnDPDP, 1 ╡mol/kg/h MnPLED (or a mixture of both) or saline. The plasma concentrations of MnDPDP, MnPLED and other metabolites (e.g., ZnDPDP and ZnPLED) were analyzed. Results: Femoral blood flow was reduced by 60% during early reperfusion in controls, whereas only 23 and 31% reductions were seen in animals treated with MnDPDP and MnPLED During that time, +LV/dP and -LV/dP (maximum rate of left ventricular isovolumic contraction and relaxation, respectively), systolic pressure and diastolic pressure fell significantly less in animals treated with MnDPDP or MnPLED. Three out of 5 control animals experienced ventricular fibrillation (VF) during reperfusion, whereas VF was not seen in any of the pigs treated with MnPLED or/and MnDPDP. The infarct sizes in saline- and MnPLED-treated animals were 39▒6 and 16▒5%, respectively, of the occluded areas. MnDPDP did not reduce the infarct size. A mixture of MnDPDP and MnPLED significantly reduced infarct size (10▒4%). When reperfusion started and throughout reperfusion, almost all injected MnDPDP was present as Zn-metabolites. Conclusion: MnPLED seems to reduce reperfusion-induced cardiac dysfunction and infarct size in pigs. MnDPDP does not reduce infarct size in the pig, probably because of the rapid exchange of Mn2+ for Zn2+ taking place in the pig.
|
|
| 5. |
|
|
