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Sökning: WFRF:(Bublin M)

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1.
  • Dramburg, S, et al. (författare)
  • EAACI Molecular Allergology User's Guide 2.0
  • 2023
  • Ingår i: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology. - 1399-3038. ; 3434 Suppl 28, s. e13854-
  • Tidskriftsartikel (refereegranskat)
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3.
  • Dubiela, P., et al. (författare)
  • Two patients with allergy to celery - Possible role of carbohydrate determinants and difference between seeds and tuber allergenicity
  • 2022
  • Ingår i: World Allergy Organization Journal. - : Elsevier BV. - 1939-4551. ; 15:11
  • Tidskriftsartikel (refereegranskat)abstract
    • Vegetables provide important nutrients but can also induce allergic symptoms. Celery tuber al-lergy frequently occurs in Central European countries and can cause allergic reactions including fatal anaphylactic shocks. There is little information about allergen content in seeds. Therefore, we analyzed 2 patients with allergic reaction after remoulade sauce consumption who entered the clinic for a diagnostic work-up. The routine diagnostic included serum derived specific IgE testing by ImmunoCAP, ImmunoCAP ISAC, and skin prick tests (SPTs). Furthermore, protein extracts were prepared from both celery tuber and celery seeds and IgE binding capacity of these extracts was assessed by immunoblots, ELISA, and rat basophil leukemia (RBL) assay. We also determined role of cross-reactive carbohydrate determinants (CCDs) by IgE inhibition ELISA. Results revealed distinct protein patterns from celery tuber and seed extracts, suggesting differences in content and quantity of allergenic proteins. IgE antibodies from both sera bound to high molecular weight (HMW) proteins on immunoblots and caused high basophil response, which was also observed upon addition of glycosylated proteins as horseradish peroxidase and Api g 5, respectively. Our results indicate that it is worth considering CCDs from plant foods as a possible allergenic factor and their contribution to the mugwort-celery syndrome.
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