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Träfflista för sökning "WFRF:(Buckingham RH) "

Sökning: WFRF:(Buckingham RH)

  • Resultat 1-10 av 16
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1.
  • Abdulkarim, Farhad, et al. (författare)
  • Missense substitutions lethal to essential functions of EF-Tu
  • 1991
  • Ingår i: Biochimie. - : Elsevier BV. - 0300-9084 .- 1638-6183. ; 73:12, s. 1457-1464
  • Tidskriftsartikel (refereegranskat)abstract
    • We have used a simple selection and screening method to isolate function defective mutants of EF-Tu. From 28 mutants tested, 12 different missense substitutions, individually lethal to some essential function of EF-Tu, were identified by sequencing. In addition we found a new non-lethal missense mutation. The frequency of isolation of unique mutations suggests that this method can be used to easily isolate many more. The lethal mutations occur in all three structural domains of EF-Tu, but most are in domain II. We aim to use these mutants to define functional domains on EF-Tu.
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2.
  • Dincbas, V, et al. (författare)
  • Shutdown in protein synthesis due to the expression of mini-genes in bacteria
  • 1999
  • Ingår i: JOURNAL OF MOLECULAR BIOLOGY. - : ACADEMIC PRESS LTD. - 0022-2836. ; 291:4, s. 745-759
  • Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
    • Mutants of Escherichia coli partially deficient in peptidyl-tRNA hydrolase are killed by the expression of certain very short open reading frames (mini-genes), encoded by the wild-type bar regions of phage lambda. According to the current hypothesis, prot
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4.
  • Freistroffer, DV, et al. (författare)
  • The accuracy of codon recognition by polypeptide release factors
  • 2000
  • Ingår i: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - : NATL ACAD SCIENCES. - 0027-8424. ; 97:5, s. 2046-2051
  • Tidskriftsartikel (refereegranskat)abstract
    • The precision with which individual termination codons in mRNA are recognized by protein release factors (RFs) has been measured and compared with the decoding of sense codons by tRNA, An Escherichia coil system for protein synthesis in vitro with purifie
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5.
  • Heurgue-Hamard, V, et al. (författare)
  • Origins of minigene-dependent growth inhibition in bacterial cells
  • 2000
  • Ingår i: EMBO JOURNAL. - : OXFORD UNIV PRESS. - 0261-4189. ; 19:11, s. 2701-2709
  • Tidskriftsartikel (refereegranskat)abstract
    • The expression of very short open reading frames in Escherichia coli can lead to the inhibition of translation and an arrest in cell growth. Inhibition occurs because peptidyl-tRNA hydrolase fails to recycle sufficiently rapidly peptidyl-tRNA released fro
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7.
  • Hughes, Diarmaid, 1956-, et al. (författare)
  • The nucleotide sequence of rpsL and its flanking regions in Salmonella typhimurium
  • 1991
  • Ingår i: Gene. - 0378-1119 .- 1879-0038. ; 104:1, s. 123-124
  • Tidskriftsartikel (refereegranskat)abstract
    • The ribosomal protein (r-protein)-encoding gene, rpsL, and regions flanking it, from Salmonella typhimurium, have been sequenced directly from polymerase chain reaction-amplified chromosomal DNA. The deduced amino acid sequence is identical to that of the Escherichia coli rpsL encoded r-protein. At the nucleotide level, the similarity is 98%, suggesting a strong pressure for the conservation of this important protein. More surprisingly, the noncoding sequences surrounding the gene are also conserved at the 98% level, suggesting that they too are functionally important.
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10.
  • Karimi, R, et al. (författare)
  • Novel roles for classical factors at the interface between translation termination and initiation
  • 1999
  • Ingår i: MOLECULAR CELL. - : CELL PRESS. - 1097-2765. ; 3:5, s. 601-609
  • Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
    • The pathway of bacterial ribosome recycling following translation termination has remained obscure, Here, we elucidate two essential steps and describe the roles played by the three translation factors EF-G, RRF, and IF3. Release factor RF3 is known to ca
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  • Resultat 1-10 av 16

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