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| 2. |
- Avoni, G., et al.
(författare)
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The new LUCID-2 detector for luminosity measurement and monitoring in ATLAS
- 2018
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Ingår i: Journal of Instrumentation. - 1748-0221. ; 13:7
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Tidskriftsartikel (refereegranskat)abstract
- The ATLAS luminosity monitor, LUCID (LUminosity Cherenkov Integrating Detector), had to be upgraded for the second run of the LHC accelerator that started in spring 2015. The increased energy of the proton beams and the higher luminosity required a redesign of LUCID to cope with the more demanding conditions. The novelty of the LUCID-2 detector is that it uses the thin quartz windows of photomultipliers as Cherenkov medium and a small amounts of radioactive 207Bi sources deposited on to these windows to monitor the gain stability of the photomultipliers. The result is a fast and accurate luminosity determination that can be kept stable during many months of data taking. LUCID-2 can also measure the luminosity accurately online for each of the up to 2808 colliding bunch pairs in the LHC . These bunch pairs are separated by only 25 ns and new electronics has been built that can count not only the number of pulses above threshold but also integrate the pulses.
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| 3. |
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| 4. |
- Cabras, T., et al.
(författare)
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Marked Differences in the Submandibular Salivary Proteome between Sardinian Alcohol-Preferring and Sardinian Alcohol-Non Preferring Rats Revealed by an Integrated Top-Down-Bottom-Up Proteomic Platform
- 2018
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 17:1, s. 455-469
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Tidskriftsartikel (refereegranskat)abstract
- Sardinian alcohol-preferring (sP) and Sardinian alcohol-non preferring (sNP) rats have been selectively bred for opposite alcohol preference and consumption. Aiming to verify possible differences at the proteomics level between sP and sNP rats, we investigated the salivary proteome by a a liquid chromatography-mass spectrometry top-down-bottom-up integrated approach. For this purpose, submandibular saliva was collected from alcohol-naive sP and sNP rats under isoprenaline stimulation. A total of 200 peptides and proteins were detected and quantified in the two rat lines, 149 of which were characterized in their naturally occurring structure. The data are available via ProteomeXchange with identifier PXD006997. Surprisingly, sP rats exhibited marked quantitative and qualitative differences with respect to sNP rats, namely higher levels of proteoforms originating from submandibular gland protein C, and from submandibular rat protein 2, as well as those of several unidentified peptides and proteins. sP rats expressed some proteins not detectable in sNP rats such as the glutamine and glutamic acid-rich protein (GRP)-CB. The isoform GRP-B, detectable in both rat lines, was more abundant in sNP rats. The submandibular saliva of sNP rats was also characterized by very high levels of GRP-B proteolytic peptides and rat salivary protein 1. Whether these differences could contribute to the opposite alcohol preference and consumption of sP and sNP rats is currently unknown and requires further investigation. © 2017 American Chemical Society.
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| 5. |
- Cabras, T., et al.
(författare)
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Top-down HPLC-ESI-MS characterization of rat gliadoralin A, a new member of the family of rat submandibular gland glutamine-rich proteins and potential substrate of transglutaminase
- 2013
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Ingår i: Journal of Separation Science. - : Wiley. - 1615-9306. ; 36:17, s. 2848-2861
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Tidskriftsartikel (refereegranskat)abstract
- During HPLC-ESI-MS/MS analysis of rat submandibular saliva secreted under isoprenaline stimulation, a protein with an experimental [M+H](1+) = 10544.24 m/z was detected (17.5 +/- 0.7 min). The MS/MS fragmentation pattern, manually investigated, allowed establishing an internal sequence in agreement with a DNA-derived sequence of an unknown rat protein coded D3Z9M3 (Swiss-Prot). To match the experimental MS/MS fragmentation pattern and protein mass with theoretical data, the removal from the N terminus of the signal peptide and from the C terminus of three amino acid (a.a.) residues (Arg-Ala-Val) and the cyclization of the N-terminal glutamine in pyroglutamic had to be supposed, resulting in a mature protein of 90 a.a. HPLC-ESI-MS/MS of the trypsin digest ensured 100% sequence coverage. For the high glutamine content (34/90 = 37.8%) we propose to name this protein rat gliadoralin A 1-90. Low amounts of five different isoforms were sporadically detected, which did not significantly change their relative amounts after stimulation. Gliadoralin A is substrate for transglutaminase-2, having Lys 60 and different Gln residues as major determinants for enzyme recognition. In silico investigation of superior structures evidenced that a small part of the protein adopts an -helical fold, whereas large segments are unfolded, suggesting an unordered conformation.
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| 6. |
- Huffman, Jennifer E., et al.
(författare)
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Modulation of Genetic Associations with Serum Urate Levels by Body-Mass-Index in Humans
- 2015
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 10:3
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Tidskriftsartikel (refereegranskat)abstract
- We tested for interactions between body mass index (BMI) and common genetic variants affecting serum urate levels, genome-wide, in up to 42569 participants. Both stratified genome-wide association (GWAS) analyses, in lean, overweight and obese individuals, and regression-type analyses in a non BMI-stratified overall sample were performed. The former did not uncover any novel locus with a major main effect, but supported modulation of effects for some known and potentially new urate loci. The latter highlighted a SNP at RBFOX3 reaching genome-wide significant level (effect size 0.014, 95% CI 0.008-0.02, P-inter= 2.6 x 10(-8)). Two top loci in interaction term analyses, RBFOX3 and ERO1LB-EDAR-ADD, also displayed suggestive differences in main effect size between the lean and obese strata. All top ranking loci for urate effect differences between BMI categories were novel and most had small magnitude but opposite direction effects between strata. They include the locus RBMS1-TANK (men, Pdifflean-overweight= 4.7 x 10(-8)), a region that has been associated with several obesity related traits, and TSPYL5 (men, Pdifflean-overweight= 9.1 x 10(-8)), regulating adipocytes-produced estradiol. The top-ranking known urate loci was ABCG2, the strongest known gout risk locus, with an effect halved in obese compared to lean men (Pdifflean-obese= 2 x 10(-4)). Finally, pathway analysis suggested a role for N-glycan biosynthesis as a prominent urate-associated pathway in the lean stratum. These results illustrate a potentially powerful way to monitor changes occurring in obesogenic environment.
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| 7. |
- Nemolato, S., et al.
(författare)
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Immunoreactivity for thymosin beta 4 and thymosin beta 10 in the adult rat oro-gastrointestinal tract
- 2013
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Ingår i: European Journal of Histochemistry. - : PAGEPress Publications. - 1121-760X .- 2038-8306. ; 57:2, s. 106-111
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Tidskriftsartikel (refereegranskat)abstract
- Thymosin beta 4 (T beta 4) and thymosin beta 10 (T beta 10) are two members of the beta-thymosin family, involved in multiple cellular activities in different organs in multiple animal species. Here we report the expression pattern of T beta 4 and T beta 10 in rat tissues, in the gut and in annexed glands. The two peptide were differently expressed: T beta 4 was absent in salivary glands whereas T beta 10 was expressed in parotid and in submandibular glands. T beta 4 was mildly expressed in the tongue and in the oesophagus, where T beta 10 was absent. A similar expression was found in the stomach, ileum and colon mucosa. In pancreas T beta 4 reactivity was restricted to the Langerhans islet cells; T beta 4 was also detected in the exocrine cells. Both peptide were not expressed in liver cells. When the rat expression pattern in rat organs was compared to reactivity for T beta 4 and T beta 10 in humans, marked differences were found. Our data clearly indicate a species-specific expression of T beta 4 and T beta 10, characterized by the actual unpredictability of the expression of these peptides in different cells and tissues. The common high expression of T beta 4 in mast cells, both in humans and in rats, represents one of the few similarities between these two species.
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