| 1. |
- Klionsky, Daniel J., et al.
(författare)
-
Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
-
Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
-
Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
|
|
| 2. |
- Arvidsson, Emma, et al.
(författare)
-
Diffusers in public ordinary rooms
- 2019
-
Ingår i: InterNoise 19, Madrid, Spain. - 9788487985317 ; , s. 5453-5461
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Traditionally, the room acoustic properties in an ordinary public room are defined by only one parameter, the reverberation time. However, research has showed that additional room acoustic parameters as sound strength and speech clarity are necessary to consider in order to achieve good acoustic conditions. In a room with acoustical ceiling treatment the effects of furniture and interiors on the room acoustic parameters are significant. In addition, by using diffusers with directional properties it is possible to individually control the room acoustic parameters. Room acoustical measurements have been carried out in a reverberation chamber with suspended absorbing ceiling and directional diffusing objects. The effect of different diffuser configurations has been examined regarding reverberation time, speech clarity and sound strength. The evaluation shows that the orientation and the amount of the diffusing elements have a significant influence on the room acoustic parameters and can be used to fine-tune the acoustical conditions in public ordinary rooms with an existing acoustic ceiling treatment. This paper presents a study showing how diffusers can be used to contribute to an improved sound environment in public ordinary rooms with an acoustic ceiling.
|
|
| 3. |
- Autio, Hanna, et al.
(författare)
-
Auralization as a tool of historic reconstruction of Vadstena Abbey church
- 2019
-
Ingår i: InterNoise 19, Madrid, Spain. - 0736-2935. - 9788487985317 ; , s. 3970-3981
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Throughout human history, there have been locations used for performance of speech and music. Many of these locations are considered part of our common cultural heritage, and more and more of them can be visited virtually as technology advances to produce better and better reconstructions. However, the technological capabilities are not the only limitations when it comes to historical reconstruction, and one of the most important research questions is often to determine the actual historical configuration of a space. This is the case for a project regarding Vadstena Abbey church in Sweden. As a step in producing the virtual reconstruction, a number of different configurations for one of the major performance locations will be examined using acoustic simulations and auralizations. As the acoustic properties of a space affects the performances within it, there should be a correspondence between the musical and oral traditions of this location in particular and its acoustics. Historical records of the performance traditions within the space, as well as records of the acoustic experiences of visitors, will be used as an evaluation tool to distinguish between the models.
|
|
