| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Lawrenson, Kate, et al.
(författare)
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Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus
- 2016
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 7
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Tidskriftsartikel (refereegranskat)abstract
- A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk.
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| 3. |
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| 4. |
- Aughton, Karen, et al.
(författare)
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hENT1 Predicts Benefit from Gemcitabine in Pancreatic Cancer but Only with Low CDA mRNA
- 2021
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Ingår i: Cancers. - : MDPI. - 2072-6694. ; 13:22
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Tidskriftsartikel (refereegranskat)abstract
- Gemcitabine or 5-fluorouracil (5-FU) based treatments can be selected for pancreatic cancer. Equilibrative nucleoside transporter 1 (hENT1) predicts adjuvant gemcitabine treatment benefit over 5-FU. Cytidine deaminase (CDA), inside or outside of the cancer cell, will deaminate gemcitabine, altering transporter affinity. ESPAC-3(v2) was a pancreatic cancer trial comparing adjuvant gemcitabine and 5-FU. Tissue microarray sections underwent in situ hybridization and immunohistochemistry. Analysis of both CDA and hENT1 was possible with 277 patients. The transcript did not correlate with protein levels for either marker. High hENT1 protein was prognostic with gemcitabine; median overall survival was 26.0 v 16.8 months (p = 0.006). Low CDA transcript was prognostic regardless of arm; 24.8 v 21.2 months with gemcitabine (p = 0.02) and 26.4 v 14.6 months with 5-FU (p = 0.02). Patients with low hENT1 protein did better with 5-FU, but only if the CDA transcript was low (median survival of 5-FU v gemcitabine; 29.3 v 18.3 months, compared with 14.2 v 14.6 with high CDA). CDA mRNA is an independent prognostic biomarker. When added to hENT1 protein status, it may also provide treatment-specific predictive information and, within the frame of a personalized treatment strategy, guide to either gemcitabine or 5FU for the individual patient.Simple Summary:Recent clinical trials suggest that combination therapies that include either gemcitabine or 5-fluorouracil (5-FU) both give significant survival benefits for pancreatic cancer patients. The tumor level of the nucleoside transporter hENT1 is prognostic in patients treated with adjuvant gemcitabine but not adjuvant 5-FU. This work shows for the first time that hENT1 is only predictive of benefit from gemcitabine over 5-FU in patients with low levels of CDA transcript. A choice between adjuvant 5-FU based combination therapies (such as FOLFIRINOX) and gemcitabine-based therapy (e.g., GemCap) could be made based on a combination of hENT1 protein and CDA mRNA measured in a resected tumor.
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| 5. |
- Axelsson, Tony, 1969, et al.
(författare)
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Theoretical debate and archaeological periodicals - incomparable?
- 1995
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Ingår i: Current Swedish Archaeology. - 1102-7355. ; 1995:3, s. 145-154
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Tidskriftsartikel (refereegranskat)abstract
- In the following article the authors will discuss the relationship between what Björn Myhre proposes as the current state of theory within Scandinavian archaeology and compare his interpretation with the issues presented in some of the Scandinavian archaeological periodicals. The discussion is based on project work carried out by students at the Dept. of Archaeology, Göteborg University. The authors of this article analyse the results of this project to see if "the post-modern period" is reflected in articles published during the period 1980-1994
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| 6. |
- Barakat-Johnson, Michelle, et al.
(författare)
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Development and Psychometric Testing of a Knowledge Instrument on Incontinence-Associated Dermatitis for Clinicians : The Know-IAD
- 2022
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Ingår i: Journal of Wound, Ostomy and Continence Nursing (WOCN). - : Lippincott Williams & Wilkins. - 1071-5754 .- 1528-3976. ; 49:1, s. 70-77
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: The purpose of this study was to describe the development and evaluation of the psychometric properties of an instrument used to assess clinician knowledge of Incontinence-associated dermatitis (IAD).Design: The instrument was developed in three phases: Phase 1 involved item development; Phase 2 evaluated content validity of the instrument by surveying clinicians and stakeholders within a single state of Australia and, Phase 3 used a pilot multisite cross-sectional survey design to determine composite reliability and evaluate scores of the knowledge tool.Subjects and settings: In Phase 1, the instrument was developed by five persons with clinical and research subject expertise in the area of IAD. In Phase 2, content validity was evaluated by a group of 13 clinicians (nurses, physicians, occupational therapists, dietitians, and physiotherapists) working in acute care across one Australian state, New South Wales, along with two consumer representatives. In Phase 3, clinicians, working across six hospitals in New South Wales and on wards with patients diagnosed with incontinence-associated dermatitis, participated in pilot-testing the instrument.Methods: During Phase 1, a group of local and international experts developed items for a draft tool based on an international consensus document, our prior research evaluating incontinence-associated dermatitis knowledge, and agreement among an expert panel of clinicians and researchers. Phase 2 used a survey design to determine content validity of the knowledge tool. Specifically, we calculated item- and scale-level content validity ratios and content validity indices for all questions within the draft instrument. Phase 3 comprised pilot-testing of the knowledge tool using a cross-sectional survey. Analysis involved confirmatory factor analysis to confirm the hypothesized model structure of the knowledge tool, as measured by model goodness-of-fit. Composite reliability testing was undertaken to determine the extent of internal consistency between constituent items of each construct.Results: During Phase 1, a draft version of the Barakat-Johnson Incontinence-Associated Dermatitis Knowledge tool (Know-IAD), comprising 19 items and divided into three domains of IAD-related knowledge: 1) Etiology and Risk, 2) Classification and Diagnosis, and 3) Prevention and Management was developed. In Phase 2, 18 of the 19 items demonstrated high scale content validity ratios scores on relevance (0.75) and clarity (0.82); and high scale-content validity indices scores on relevance (0.87) and clarity (0.91). In Phase 3, the final 18-item Know-IAD tool demonstrated construct validity by a model goodness-of-fit. Construct validity was excellent for the Etiology and Risk domain (root mean squared error=0.02) and Prevention and Management domain (root mean squared error=0.02); it was good for the Classification and Diagnosis domain (root mean squared error=0.04). Composite reliability (CR) was good in the Etiology and Risk domain (CR=0.76), Prevention and Management domains (CR=0.75), and adequate in the Classification and Diagnosis domain (CR=0.64). Respondents had good understanding of etiology and risk (72.6% correct responses); fairly good understanding of prevention and management of IAD (64.0% correct responses) and moderate understanding of classification and diagnosis (40.2% correct responses).Conclusions: The Know-IAD demonstrated good psychometric properties and provides preliminary evidence that it can be applied to evaluate clinician knowledge on IAD.
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| 7. |
- Campbell, Fiona, 1961, et al.
(författare)
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Beyond culture : encounters with otherness
- 1997
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Ingår i: The interpretation of culture and the culture of interpretation : proceedings from the first graduate conference at the Department of Literature in Uppsala, April 20-21 1996. Editors: Eva Hemmungs Wirtén and Erik Peurell (Skrifter utgivna av Avdelningen för litteratursociologi vid Litteraturvetenskapliga institutionen i Uppsala, 36). - 9185178276 ; , s. 133-150
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Konferensbidrag (refereegranskat)
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| 8. |
- Campbell, Fiona, 1961, et al.
(författare)
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Borderline archaeology : a practice of contemporary archaeology - exploring aspects of creative narratives and performative cultural production
- 2004
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This dissertation is a joint dissertation, written by two people about the connectivity of two practices; archaeology and performance. Its contents focus upon the creation of a hybrid field of study that has only just begun to exist. We have named this dissertation 'BorderLine Archaeology' because we feel that this title reveals the relevance of its position as a field of study that is geographically situated on the border, on the line where things and people meet, at a borderline which is in a constant state of negotiation, and change. 'BorderLine Archaeology' is the site that bridges the gap between art and academia; it is the meeting place where subjective experience has a central role in the exploration of alternative ways to approach archaeological objects in the context of belonging to a process of cultural production. The purpose of this dissertation is: to create a theoretical methodology of BorderLine Archaeology, that provides alternative strategies to use when dealing with archaeological matters; to explore the process of performative writing as an alternative approach in the re-presentation of the archaeological; to investigate archaeology's potential as a mode performative cultural production and to produce a body of knowledge, a kind of archaeology that is theoretical yet practical, that is hybrid, sensorial, inter-subjective, multilayered and performative. The aims and objectives of this dissertation are approached through the co-authored chapters, 'Frontwords', 'Framework', 'Proposition', 'Making our Way' and 'Afterwords', where we set the context, create a theoretical methodology and sum up our work. But they are also approached through the production of two separate case-studies, where we implement the theoretical methodology of BorderLine Archaeology and use the process of performative writing in order to reveal its potential. In the case-study 'Turning 180 into the Walkscape of the Labyrinth' Fiona Campbell presents the labyrinths of Sweden and investigates how the act of moving affects the way we experience, perceive and re-present the past-present. In the case-study 'Turning into the Walkscape of the Family' Jonna Ulin deliberates the complexities of postmemory through an exploration of the family landscape as a site of the archaeological, as a site that needs to be interpreted through a process of reading onto and into. Both case-studies are connected to a co-produced website http://arkserv.arch.gu.se/blalab, where the source materials for the separate projects are stored. The website provides the additional perspectives to the written texts, inviting the visitor to explore further into the world of the Swedish labyrinths and the world of the family landscape. Some outcomes of this dissertation are; that the practice of BorderLine Archaeology is an embodied, inter-subjective process of reading, writing, interpreting and witnessing archaeological matters; that it is a practice of overlapping discourse, of crossing borders whilst moving the experiences of the participants onto and into the matters of everyday, into the place of the familiar, unfamiliar, the unspeakable, the silent, the same and other.
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| 9. |
- Campbell, Fiona, 1961, et al.
(författare)
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Identitet bortom kultur : ett möte med "det Andra"
- 1998
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Ingår i: Arkeologiska horisonter. Ola W. Jensen & Håkan Karlsson (red.). - : Stockholm : Symposion. - 9171394117 ; , s. 46-63
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 10. |
- Clark, Christopher E., et al.
(författare)
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Associations Between Systolic Interarm Differences in Blood Pressure and Cardiovascular Disease Outcomes and Mortality : Individual Participant Data Meta-Analysis, Development and Validation of a Prognostic Algorithm: The INTERPRESS-IPD Collaboration
- 2021
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Ingår i: Hypertension. - 1524-4563. ; 77:2, s. 650-661
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Tidskriftsartikel (refereegranskat)abstract
- Systolic interarm differences in blood pressure have been associated with all-cause mortality and cardiovascular disease. We undertook individual participant data meta-analyses to (1) quantify independent associations of systolic interarm difference with mortality and cardiovascular events; (2) develop and validate prognostic models incorporating interarm difference, and (3) determine whether interarm difference remains associated with risk after adjustment for common cardiovascular risk scores. We searched for studies recording bilateral blood pressure and outcomes, established agreements with collaborating authors, and created a single international dataset: the Inter-arm Blood Pressure Difference - Individual Participant Data (INTERPRESS-IPD) Collaboration. Data were merged from 24 studies (53 827 participants). Systolic interarm difference was associated with all-cause and cardiovascular mortality: continuous hazard ratios 1.05 (95% CI, 1.02-1.08) and 1.06 (95% CI, 1.02-1.11), respectively, per 5 mm Hg systolic interarm difference. Hazard ratios for all-cause mortality increased with interarm difference magnitude from a ≥5 mm Hg threshold (hazard ratio, 1.07 [95% CI, 1.01-1.14]). Systolic interarm differences per 5 mm Hg were associated with cardiovascular events in people without preexisting disease, after adjustment for Atherosclerotic Cardiovascular Disease (hazard ratio, 1.04 [95% CI, 1.00-1.08]), Framingham (hazard ratio, 1.04 [95% CI, 1.01-1.08]), or QRISK cardiovascular disease risk algorithm version 2 (QRISK2) (hazard ratio, 1.12 [95% CI, 1.06-1.18]) cardiovascular risk scores. Our findings confirm that systolic interarm difference is associated with increased all-cause mortality, cardiovascular mortality, and cardiovascular events. Blood pressure should be measured in both arms during cardiovascular assessment. A systolic interarm difference of 10 mm Hg is proposed as the upper limit of normal. Registration: URL: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42015031227.
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