SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Cao Peng) "

Sökning: WFRF:(Cao Peng)

  • Resultat 1-10 av 425
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
  •  
2.
  • 2019
  • Tidskriftsartikel (refereegranskat)
  •  
3.
  •  
4.
  •  
5.
  •  
6.
  •  
7.
  • Zhu, RJ, et al. (författare)
  • Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms
  • 2021
  • Ingår i: Cell research. - : Springer Science and Business Media LLC. - 1748-7838 .- 1001-0602. ; 31:12, s. 1244-1262
  • Tidskriftsartikel (refereegranskat)abstract
    • The infusion of coronavirus disease 2019 (COVID-19) patients with mesenchymal stem cells (MSCs) potentially improves clinical symptoms, but the underlying mechanism remains unclear. We conducted a randomized, single-blind, placebo-controlled (29 patients/group) phase II clinical trial to validate previous findings and explore the potential mechanisms. Patients treated with umbilical cord-derived MSCs exhibited a shorter hospital stay (P = 0.0198) and less time required for symptoms remission (P = 0.0194) than those who received placebo. Based on chest images, both severe and critical patients treated with MSCs showed improvement by day 7 (P = 0.0099) and day 21 (P = 0.0084). MSC-treated patients had fewer adverse events. MSC infusion reduced the levels of C-reactive protein, proinflammatory cytokines, and neutrophil extracellular traps (NETs) and promoted the maintenance of SARS-CoV-2-specific antibodies. To explore how MSCs modulate the immune system, we employed single-cell RNA sequencing analysis on peripheral blood. Our analysis identified a novel subpopulation of VNN2+ hematopoietic stem/progenitor-like (HSPC-like) cells expressing CSF3R and PTPRE that were mobilized following MSC infusion. Genes encoding chemotaxis factors — CX3CR1 and L-selectin — were upregulated in various immune cells. MSC treatment also regulated B cell subsets and increased the expression of costimulatory CD28 in T cells in vivo and in vitro. In addition, an in vivo mouse study confirmed that MSCs suppressed NET release and reduced venous thrombosis by upregulating kindlin-3 signaling. Together, our results underscore the role of MSCs in improving COVID-19 patient outcomes via maintenance of immune homeostasis.
  •  
8.
  •  
9.
  •  
10.
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 1-10 av 425
Typ av publikation
tidskriftsartikel (418)
konferensbidrag (3)
annan publikation (1)
forskningsöversikt (1)
Typ av innehåll
refereegranskat (419)
övrigt vetenskapligt/konstnärligt (4)
Författare/redaktör
Xu, L. (367)
Wang, K. (366)
Peters, K. (365)
Cetin, S. A. (363)
Jin, S. (363)
Ouyang, Q. (362)
visa fler...
Zhemchugov, A. (361)
Cakir, O. (358)
Liu, K. (355)
Zhang, L. (353)
Zhou, L. (338)
Fang, Y. (330)
Liu, J. B. (326)
Chen, X. (324)
Liu, D. (315)
Zhang, J. (301)
Yang, Y. (289)
Huang, Y. (288)
Berger, N. (282)
Liu, B. (278)
Chen, S. (256)
Bai, Y. (253)
Zhu, J. (253)
Werner, M. (232)
Wang, J. (225)
Li, H. (224)
Liu, Y. (224)
Wang, R. (224)
Chen, H. (223)
Li, B. (223)
Wang, H. (223)
Zhang, X. (223)
Chen, C. (222)
Chen, Y. (222)
Francis, D. (222)
Liu, M. (222)
Sanchez, A. (222)
Silva, J. (222)
White, S. (222)
Williams, S. (222)
Wilson, A. (222)
Zhu, Y. (222)
Duda, D. (221)
Peng, H. (221)
Sun, X. (221)
Vrba, V. (221)
Walker, R. (221)
Wang, C. (221)
Zhang, Z. (221)
Zimmermann, S. (221)
visa färre...
Lärosäte
Uppsala universitet (346)
Lunds universitet (229)
Kungliga Tekniska Högskolan (211)
Stockholms universitet (209)
Karolinska Institutet (24)
Chalmers tekniska högskola (8)
visa fler...
Linköpings universitet (7)
Göteborgs universitet (6)
Umeå universitet (4)
Sveriges Lantbruksuniversitet (3)
Örebro universitet (2)
Högskolan i Halmstad (1)
Mälardalens universitet (1)
Högskolan Dalarna (1)
visa färre...
Språk
Engelska (425)
Forskningsämne (UKÄ/SCB)
Naturvetenskap (391)
Medicin och hälsovetenskap (14)
Teknik (13)

År

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy