| 1. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 2. |
- Laurent, Timothée, et al.
(författare)
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Architecture of the chikungunya virus replication organelle
- 2022
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Ingår i: eLIFE. - : eLife Sciences Publications. - 2050-084X. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- Alphaviruses are mosquito-borne viruses that cause serious disease in humans and other mammals. Along with its mosquito vector, the Alphavirus chikungunya virus (CHIKV) has spread explosively in the last 20 years, and there is no approved treatment for chikungunya fever. On the plasma membrane of the infected cell, CHIKV generates dedicated organelles for viral RNA replication, so-called spherules. Whereas structures exist for several viral proteins that make up the spherule, the architecture of the full organelle is unknown. Here, we use cryo-electron tomography to image CHIKV spherules in their cellular context. This reveals that the viral protein nsP1 serves as a base for the assembly of a larger protein complex at the neck of the membrane bud. Biochemical assays show that the viral helicase-protease nsP2, while having no membrane affinity on its own, is recruited to membranes by nsP1. The tomograms further reveal that full-sized spherules contain a single copy of the viral genome in double-stranded form. Finally, we present a mathematical model that explains the membrane remodeling of the spherule in terms of the pressure exerted on the membrane by the polymerizing RNA, which provides a good agreement with the experimental data. The energy released by RNA polymerization is found to be sufficient to remodel the membrane to the characteristic spherule shape.
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| 3. |
- Moberg, Christina, et al.
(författare)
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De unga gör helt rätt när de stämmer staten
- 2022
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Ingår i: Aftonbladet. - 1103-9000.
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- 1 620 forskare och lärare i forskarvärlden: Vi ställer oss bakom Auroras klimatkrav
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| 4. |
- Swift, Imogen J, et al.
(författare)
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A systematic review of progranulin concentrations in biofluids in over 7,000 people-assessing the pathogenicity of GRN mutations and other influencing factors.
- 2024
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Ingår i: Alzheimer's Research & Therapy. - 1758-9193. ; 16:1
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Tidskriftsartikel (refereegranskat)abstract
- Pathogenic heterozygous mutations in the progranulin gene (GRN) are a key cause of frontotemporal dementia (FTD), leading to significantly reduced biofluid concentrations of the progranulin protein (PGRN). This has led to a number of ongoing therapeutic trials aiming to treat this form of FTD by increasing PGRN levels in mutation carriers. However, we currently lack a complete understanding of factors that affect PGRN levels and potential variation in measurement methods. Here, we aimed to address this gap in knowledge by systematically reviewing published literature on biofluid PGRN concentrations.Published data including biofluid PGRN concentration, age, sex, diagnosis and GRN mutation were collected for 7071 individuals from 75 publications. The majority of analyses (72%) had focused on plasma PGRN concentrations, with many of these (56%) measured with a single assay type (Adipogen) and so the influence of mutation type, age at onset, sex, and diagnosis were investigated in this subset of the data.We established a plasma PGRN concentration cut-off between pathogenic mutation carriers and non-carriers of 74.8ng/mL using the Adipogen assay based on 3301 individuals, with a CSF concentration cut-off of 3.43ng/mL. Plasma PGRN concentration varied by GRN mutation type as well as by clinical diagnosis in those without a GRN mutation. Plasma PGRN concentration was significantly higher in women than men in GRN mutation carriers (p=0.007) with a trend in non-carriers (p=0.062), and there was a significant but weak positive correlation with age in both GRN mutation carriers and non-carriers. No significant association was seen with weight or with TMEM106B rs1990622 genotype. However, higher plasma PGRN levels were seen in those with the GRN rs5848 CC genotype in both GRN mutation carriers and non-carriers.These results further support the usefulness of PGRN concentration for the identification of the large majority of pathogenic mutations in the GRN gene. Furthermore, these results highlight the importance of considering additional factors, such as mutation type, sex and age when interpreting PGRN concentrations. This will be particularly important as we enter the era of trials for progranulin-associated FTD.
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| 5. |
- Andersson, Björn O, 1982, et al.
(författare)
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A Modified TAB Model for Simulation of Atomization in Rotary Bell Spray Painting
- 2013
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Ingår i: Journal of Mechanical Engineering and Automation. - 2163-2413. ; 3:2, s. 54-61
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Tidskriftsartikel (refereegranskat)abstract
- The Rotary bell spray applicator technique is commonly used in the automotive industry for paint application because of its high transfer efficiency and high-quality result. The bell spins rapidly around its axis with a tangential velocity at the edge in the order of 100 m/s. The paint falls off the edge and enters the air with a large relative velocity, driving the atomization into small droplets where the resulting size distribution depends on the process conditions. Especially the rotation speed of the bell is an important parameter governing the size distribution. The main research question in this work is to investigate if the Taylor Analogy Breakup (TAB) model can be used to predict the resulting droplet size distributions in spray painting. As the paint is a viscous fluid a modification of the TAB model taking non-linear effects of large viscosity into account is proposed. The parameters in the breakup model are tuned by optimization to match droplet size distributions obtained in CFD simulations with measured ones. Results are presented for three cases with rotation speeds from 30 to 50 thousand RPM where the full droplet size distributions are compared with measurements. Good results are obtained for all three cases where the simulated size distributions compare well to measurements over a wide range of droplet sizes. The TAB method is able to quantitatively predict the result of the breakup process and can be used in a preprocessing stage of a full spray painting simulation, thereby reducing the need for costly and cumbersome measurements.
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| 6. |
- Andersson, Björn O, 1982, et al.
(författare)
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Modified TAB Model for Viscous Fluids applied to Breakup in Rotary Bell Spray Painting
- 2012
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Ingår i: ICLASS 2012 - 12th International Conference on Liquid Atomization and Spray Systems.
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Konferensbidrag (refereegranskat)abstract
- The Taylor Analogy Breakup (TAB) model is applied to droplet breakup in rotary bell spray painting commonly used in the automotive industry. The bell spins rapidly around its axis with a tangential velocity at the edge in the order of 100 m/s. The paint falls off the edge and enters the air with a large relative velocity, driving the atomization. The paint is a viscous fluid and a modification of the TAB model taking non-linear effects of large viscosity into account is described. The parameters in the breakup model are tuned to match droplet size distributions obtained in CFD simulations with measured ones. Results are presented for three cases with rotation speeds from 30 to 50 thousand RPM where the full droplet size distributions are compared with measurements. Good results are obtained for all three cases where the simulated size distributions compare well to measurements over a wide range of droplet sizes. The obtained results can be used in a preprocessing stage of a full spray painting simulation thereby reducing the need for costly and cumbersome measurements.
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| 7. |
- Carlson, Andreas, 1981-
(författare)
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Capillarity and dynamic wetting
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In this thesis capillary dominated two–phase flow is studied by means of nu- merical simulations and experiments. The theoretical basis for the simulations consists of a phase field model, which is derived from the system’s thermody- namics, and coupled with the Navier Stokes equations. Two types of interfacial flow are investigated, droplet dynamics in a bifurcating channel and sponta- neous capillary driven spreading of drops.Microfluidic and biomedical applications often rely on a precise control of droplets as they traverse through complicated networks of bifurcating channels. Three–dimensional simulations of droplet dynamics in a bifurcating channel are performed for a set of parameters, to describe their influence on the resulting droplet dynamics. Two distinct flow regimes are identified as the droplet in- teracts with the tip of the channel junction, namely, droplet splitting and non- splitting. A flow map based on droplet size and Capillary number is proposed to predict whether the droplet splits or not in such a geometry.A commonly occurring flow is the dynamic wetting of a dry solid substrate. Both experiments and numerical simulations of the spreading of a drop are presented here. A direct comparison of the two identifies a new parameter in the phase field model that is required to accurately predict the experimental spreading behavior. This parameter μf [P a · s], is interpreted as a friction factor at the moving contact line. Comparison of simulations and experiments for different liquids and surface wetting properties enabled a measurement of the contact line friction factor for a wide parameter space. Values for the contact line friction factor from phase field theory are reported here for the first time.To identify the physical mechanism that governs the droplet spreading, the different contributions to the flow are measured from the simulations. An im- portant part of the dissipation may arise from a friction related to the motion of the contact line itself, and this is found to be dominating both inertia and viscous friction adjacent to the contact line. A scaling law based on the con- tact line friction factor collapses the experimental data, whereas a conventional inertial or viscous scaling fails to rationalize the experimental observation, supporting the numerical finding.
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| 8. |
- Carlson, Andreas, et al.
(författare)
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Contact line dissipation in short-time dynamic wetting
- 2012
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Ingår i: Europhysics letters. - : IOP Publishing. - 0295-5075 .- 1286-4854. ; 97:4
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Tidskriftsartikel (refereegranskat)abstract
- Dynamic wetting of a solid surface is a process that is ubiquitous in Nature, and also of increasing technological importance. The underlying dissipative mechanisms are, however, still unclear. We present here short-time dynamic wetting experiments and numerical simulations, based on a phase field approach, of a droplet on a dry solid surface, where direct comparison of the two allows us to evaluate the different contributions from the numerics. We find that an important part of the dissipation may arise from a friction related to the motion of the contact line itself, and that this may be dominating both inertia and viscous friction in the flow adjacent to the contact line. A contact line friction factor appears in the theoretical formulation that can be distinguished and quantified, also in room temperature where other sources of dissipation are present. Water and glycerin-water mixtures on various surfaces have been investigated where we show the dependency of the friction factor on the nature of the surface, and the viscosity of the liquid.
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| 9. |
- Carlson, Andreas, et al.
(författare)
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Dissipation in rapid dynamic wetting
- 2011
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Ingår i: Journal of Fluid Mechanics. - : Cambridge University Press (CUP). - 0022-1120 .- 1469-7645. ; 682, s. 213-240
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Tidskriftsartikel (refereegranskat)abstract
- In this article, we present a modelling approach for rapid dynamic wetting based on the phase field theory. We show that in order to model this accurately, it is important to allow for a non-equilibrium wetting boundary condition. Using a condition of this type, we obtain a direct match with experimental results reported in the literature for rapid spreading of liquid droplets on dry surfaces. By extracting the dissipation of energy and the rate of change of kinetic energy in the flow simulation, we identify a new wetting regime during the rapid phase of spreading. This is characterized by the main dissipation to be due to a re-organization of molecules at the contact line, in a diffusive or active process. This regime serves as an addition to the other wetting regimes that have previously been reported in the literature.
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| 10. |
- Carlson, Andreas, et al.
(författare)
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Droplet dynamics in a bifurcating channel
- 2010
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Ingår i: International Journal of Multiphase Flow. - : Elsevier BV. - 0301-9322 .- 1879-3533. ; 36:5, s. 397-405
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Tidskriftsartikel (refereegranskat)abstract
- In the present paper we present a phenomenological description of droplet dynamics in a bifurcating channel that is based on three-dimensional numerical experiments using the Phase Field theory. Droplet dynamics is investigated in a junction, which has symmetric outflow conditions in its daughter branches. We identify two different flow regimes as the droplets interact with the tip of the bifurcation, splitting and non-splitting. A distinct criterion for the flow regime transition is found based on the initial droplet volume and the Capillary (Ca) number. The Rayleigh Plateau instability is identified as a driving mechanism for the droplet breakup close to the threshold between the splitting and non-splitting regime.
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