| 1. |
- Gabrielsson, Britt, 1957, et al.
(författare)
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Depot-specific expression of fibroblast growth factors in human adipose tissue.
- 2002
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Ingår i: Obesity research. - : Wiley. - 1071-7323 .- 1550-8528. ; 10:7, s. 608-16
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Tidskriftsartikel (refereegranskat)abstract
- We have investigated the expression of several fibroblast growth factors (FGFs) and FGF-receptors (FGFRs) in human adipose tissue and adipose-tissue cell fractions obtained from both subcutaneous (sc) and omental (om) depots.
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| 2. |
- Gabrielsson, Britt, 1957, et al.
(författare)
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High expression of complement components in omental adipose tissue in obese men.
- 2003
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Ingår i: Obesity research. - : Wiley. - 1071-7323 .- 1550-8528. ; 11:6, s. 699-708
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: Accumulation of visceral fat is recognized as a predictor of obesity-related metabolic disturbances. Factors that are predominantly expressed in this depot could mediate the link between visceral obesity and associated diseases. RESEARCH METHODS AND PROCEDURES: Paired subcutaneous and omental adipose tissue biopsies were obtained from 10 obese men. Gene expression was analyzed by DNA microarrays in triplicate and by real-time polymerase chain reaction. Serum C3 and C4 were analyzed by radial immunodiffusion assays in 91 subjects representing a cross section of the general population. Body composition was measured by computerized tomography. RESULTS: Complement components C2, C3, C4, C7, and Factor B had higher expression in omental compared with subcutaneous adipose tissue ( approximately 2-, 4-, 17-, 10-, and 7-fold, respectively). In addition, adipsin, which belongs to the alternative pathway, and the classical pathway components C1QB, C1R, and C1S were expressed in both depots. Analysis of tissue distribution showed high expression of C2, C3, and C4 in omental adipose tissue, and only liver had higher expression of these genes. Serum C3 levels correlated with both visceral and subcutaneous adipose tissue in both men (r = 0.65 and p < 0.001 and r = 0.52 and p < 0.001, respectively) and women (r = 0.34 and p = 0.023 and r = 0.49 and p < 0.001, respectively), whereas C4 levels correlated with only visceral fat in men (r = 0.36, p = 0.015) and with both depots in women (visceral: r = 0.58, p < 0.001; and subcutaneous: r = 0.51, p < 0.001). DISCUSSION: Recent studies show that the metabolic syndrome is associated with chronically elevated levels of several immune markers, some of which may have metabolic effects. The high expression of complement genes in intra-abdominal adipose tissue might suggest that the complement system is involved in the development of visceral adiposity and/or contributes to the metabolic complications associated with increased visceral fat mass.
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| 3. |
- Gabrielsson, Britt, 1957, et al.
(författare)
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Molecular characterization of a local sulfonylurea system in human adipose tissue.
- 2004
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Ingår i: Molecular and cellular biochemistry. - 0300-8177 .- 1573-4919. ; 258:1-2, s. 65-71
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Tidskriftsartikel (refereegranskat)abstract
- ATP-sensitive potassium (KATP) channels are present in many cell types and link cellular metabolism to the membrane potential. These channels are heterooctamers composed of two subunits. The sulfonylurea receptor (SUR) subunits are targets for drugs that are inhibitors or openers of the KATP channels, while the inwardly rectifying K+ (Kir) subunits form the ion channel. Two different SUR genes (SUR1 and SUR2) and two different Kir6.x genes (Kir6.1 and Kir6.2) have been identified. In addition, isoforms of SUR2, SUR2A and SUR2B, have been described. We have previously performed expression profiling on pooled human adipose tissue and found high expression of SUR2. Others have reported expression of SUR1 in human adipocytes. The aim of this study was to characterize the expression of the sulfonylurea receptor complex components in human adipose tissue. RT-PCR analysis, verified by restriction enzyme digestions and DNA sequencing, showed that SUR2B, Kir6.1 and alpha-endosulfine, but not SUR1, SUR2A or Kir6.2, are expressed in human adipose tissue. Real-time RT-PCR showed that SUR2B was expressed at higher levels in subcutaneous compared with omental adipose tissue in paired biopsies obtained from seven obese men (p < 0.05). Analysis of tissue distribution showed that SUR2B expression in adipose tissue was lower than that in muscle, similar to that in heart and liver, while the expression in pancreas was lower. The effect of caloric restriction was tested in obese men (n = 10) treated with very low calorie diet for 16 weeks, followed by a gradual reintroduction of ordinary food for 2 weeks. Biopsies were taken at week 0, 8 and 18. There was no consistent effect of weight reduction on SUR2B or Kir6.1 expression. We conclude that the necessary components for a local sulfonylurea system are expressed in human adipose tissue and that the sulfonylurea receptor complex in this tissue is composed of SUR2B and Kir6.1. The expression of SUR2B was higher in subcutaneous compared with omental adipose tissue and was not affected by weight loss.
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| 4. |
- Carlsson, Magnus, et al.
(författare)
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The acute effects of a short technique-intense training period on side-foot kick performance among elite female soccer players
- 2019
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Ingår i: Journal of Sports Medicine and Physical Fitness. - 0022-4707 .- 1827-1928. ; 59:9, s. 1442-1449
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Previously, it was shown that elite soccer teams were 24% more likely to win matches if their passing effectiveness were increased by 1%. However, research interventions aiming to improve passing performance are scarce. The current study aimed to investigate the effect of a short technique-intense training period on side-foot kick performance among elite female soccer players.METHODS: Four side-foot kick tests were completed before and after a training period: kicking a stationary ball using match-relevant (SBRS) and maximal ball speed (SBMS), passing the ball on the move using match-relevant ball speed (RBRS), and repeated side-foot kicks onto a rebound-box with continuously increasing passing distance (RRB). The players were assigned to either the intervention group or the control group. The training intervention consisted of six 55-min training sessions with five side-foot kick exercises. Within-group and between-group differences were investigated using paired-samples t-test and Mann-Whitney U test, respectively.RESULTS: The intervention group improved the performance in the RBRS and RRB tests (both P < 0.05), but no differences were found for the SBRS and SBMS tests (both P > 0.05). No improvements were found for the control group independent of test condition (all P > 0.05). Significant between-group differences were found for the RBRS and RRB tests (both P < 0.05), whereas no differences were found for the SBRS and SBMS tests (both P > 0.05).CONCLUSIONS: The fundamental soccer skill of passing a moving ball was improved in elite female soccer players by a short technique-intense training period.
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| 5. |
- Carlsson, Magnus, et al.
(författare)
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The effect of training on side foot-kick performance among swedish first league women´s soccer players
- 2018
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Konferensbidrag (refereegranskat)abstract
- THE EFFECT OF TRAINING ON SIDE FOOT-KICK PERFORMANCE AMONG SWEDISH FIRST LEAGUE WOMEN’S SOCCER PLAYERSCarlsson, M.1, Isberg, J.1, Nilsson, J.1, Carlsson, T.1 1: Dalarna University (Falun, Sweden)IntroductionA high completion rate for passes is important for success in soccer, because longer passing sequences are related to more scored goals (Hughes & Franks, 2005). In a recent study, it was found that female players had a lower pass-completion rate than male players at the highest competitive standard of European soccer, which suggests that elite female players in general do not have the same technical characteristics as elite male players (Paul S. Bradley et al., 2014). The purpose of the study was investigate the effect of a 2-week training intervention on side foot-kick performance among Swedish first league women’s soccer players. MethodsTo investigate the effect of training on side foot-kick performance, a pre-post-intervention study was implemented where four side foot-kick tests were performed before and after a 2-week training period. The side foot-kick accuracy were investigated when kicking a stationary ball using match-relevant ball speed (SBRS) and maximal ball speed (SBMS) as well as subsequent to a 5-m run with the ball from different approach angles (0°, 30°, and 60°) to a predetermined position, where passing of the ball on the move was executed using match-relevant ball speed (RBRS). The fourth test comprised repeated side-foot kicks onto a rebound-box with continuously increasing passing distance (RRB).Based on the results from the pre-tests, the players were assigned to either the intervention group (INT) or the control group (CON). The training intervention consisted of six 55-min training sessions. In each session, two rounds of five exercises focusing on improvement of side foot-kick accuracy were executed. Within-group and between-group differences were investigated using paired samples Student’s t-tests and Mann-Whitney U tests, respectively.ResultsPrior to the training intervention, there were no significant differences between the groups for any of the investigated test variables. The INT group improved RBRS (P = 0.036) and RRB (P = 0.010) during the training intervention, whereas no significant within-group changes were found for either SBRS or SBMS (both P > 0.05). No within-group differences were found for any of the test variables in the CON group (all P > 0.05). Significant between-group differences were found for RBRS (P = 0.040) and RRB (P = 0.005), whereas no differences were found for either SBRS or SBMS (both P > 0.05).ConclusionThe fundamental soccer skill of passing a moving ball could be improved in elite women players by a 2-week training period focusing on improving side foot-kick performance.ReferencesBradley PS, Carling C, Diaz AG, Hood P, Barnes C, Ade J, Boddy M, Krustrup P, Mohr M (2013) Hum Mov Sci, 32, 808-821.Hughes M, Franks I (2005) J Sports Sci, 23, 509-514.
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| 6. |
- Carlsson, Susanne, et al.
(författare)
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Affinity of galectin-8 and its carbohydrate recognition domains for ligands in solution and at the cell surface.
- 2007
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Ingår i: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 17:6, s. 663-76
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Tidskriftsartikel (refereegranskat)abstract
- Galectin-8 has two different carbohydrate recognition domains (CRDs), the N-terminal Gal-8N and the C-terminal Gal-8C linked by a peptide, and has various effects on cell adhesion and signaling. To understand the mechanism for these effects further, we compared the binding activities of galectin-8 in solution with its binding and activation of cells. We used glycan array analysis to broaden the specificity profile of the two galectin-8 CRDs, as well as intact galectin-8s (short and long linker), confirming the unique preference for sulfated and sialylated glycans of Gal-8N. Using a fluorescence anisotropy assay, we examined the solution affinities for a subset of these glycans, the highest being 50 nM for NeuAcalpha2,3Lac by Gal-8N. Thus, carbohydrate-protein interactions can be of high affinity without requiring multivalency. More importantly, using fluorescence polarization, we also gained information on how the affinity is built by multiple weak interactions between different fragments of the glycan and its carrier molecule and the galectin CRD subsites (A-E). In intact galectin-8 proteins, the two domains act independently of each other in solution, whereas at a surface they act together. Ligands with moderate or weak affinity for the isolated CRDs on the array are bound strongly by intact galectin-8s. Also galectin-8 binding and signaling at cell surfaces can be explained by combined binding of the two CRDs to low or medium affinity ligands, and their highest affinity ligands, such as sialylated galactosides, are not required.
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| 7. |
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| 8. |
- Hesson, Jenny C., et al.
(författare)
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Culex torrentium Mosquito Role as Major Enzootic Vector Defined by Rate of Sindbis Virus Infection, Sweden, 2009
- 2015
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Ingår i: Emerging Infectious Diseases. - : Centers for Disease Control and Prevention (CDC). - 1080-6040 .- 1080-6059. ; 21:5, s. 875-878
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Tidskriftsartikel (refereegranskat)abstract
- We isolated Sindbis virus (SINV) from the enzootic mosquito vectors Culex torrentium, Cx. pipiens, and Culiseta morsitans collected in an area of Sweden where SINV disease is endemic. The infection rate in Cx. torrentium mosquitoes was exceptionally high (36 infections/1,000 mosquitoes), defining Cx. torrentium as the main enzootic vector of SINV in Scandinavia.
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