| 1. |
- Cardellini, Jacopo, et al.
(författare)
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Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics
- 2023
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Ingår i: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797. ; 640, s. 100-109
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Tidskriftsartikel (refereegranskat)abstract
- Although promising for biomedicine, the clinical translation of inorganic nanoparticles (NPs) is limited by low biocompatibility and stability in biological fluids. A common strategy to circumvent this drawback consists in disguising the active inorganic core with a lipid bilayer coating, reminiscent of the structure of the cell membrane to redefine the chemical and biological identity of NPs. While recent reports introduced membrane-coating procedures for NPs, a robust and accessible method to quantify the integrity of the bilayer coverage is not yet available. To fill this gap, we prepared SiO2 nanoparticles (SiO2NPs) with different membrane coverage degrees and monitored their interaction with AuNPs by combining microscopic, scattering, and optical techniques. The membrane-coating on SiO2NPs induces spontaneous clustering of AuNPs, whose extent depends on the coating integrity. Remarkably, we discovered a linear correlation between the membrane coverage and a spectral descriptor for the AuNPs’ plasmonic resonance, spanning a wide range of coating yields. These results provide a fast and cost-effective assay to monitor the compatibilization of NPs with biological environments, essential for bench tests and scale-up. In addition, we introduce a robust and scalable method to prepare SiO2NPs/AuNPs hybrids through spontaneous self-assembly, with a high-fidelity structural control mediated by a lipid bilayer.
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| 2. |
- Martens, Pim, et al.
(författare)
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New Directions in Globalization Indices
- 2015
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Ingår i: Globalizations. - : Informa UK Limited. - 1474-7731 .- 1474-774X. ; 12:2, s. 217-228
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Tidskriftsartikel (refereegranskat)abstract
- This paper discusses the measurement of globalization with a view to advancing the construction of globalization indices. It critically analyzes the types of indices that can contribute to knowledge and policy on globalization. Three issues are particularly highlighted: (a) the focus of measurement (i.e. on activities or policies); (b) the dimensions of measurement (i.e. cultural, ecological, economic, political, and/or social); and (c) the units of measurement (i.e. local, national, regional, and/or global). This paper argues that a workable forward strategy should not seek to identify the single best composite globalization index, but rather should work in an interdisciplinary mode towards a set of complementary globalization indices. These quantitative analyses can then be productively blended with qualitative approaches in a fuller assessment of globalization's extent and impact.
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| 3. |
- Ridolfi, Andrea, et al.
(författare)
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Nanoscale structural and mechanical characterization of thin bicontinuous cubic phase lipid films
- 2022
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Ingår i: Colloids and Surfaces B: Biointerfaces. - : Elsevier BV. - 0927-7765. ; 210
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Tidskriftsartikel (refereegranskat)abstract
- The mechanical response of lipid membranes to nanoscale deformations is of fundamental importance for understanding how these interfaces behave in multiple biological processes; in particular, the nanoscale mechanics of non-lamellar membranes represents a largely unexplored research field. Among these mesophases, inverse bicontinuous cubic phase QII membranes have been found to spontaneously occur in stressed or virally infected cells and to play a role in fundamental processes, such as cell fusion and food digestion. We herein report on the fabrication of thin ( ̴150 nm) supported QII cubic phase lipid films (SQIIFs) and on their characterization via multiple techniques including Small Angle X-Ray Scattering (SAXS), Ellipsometry and Atomic Force Microscopy (AFM). Moreover, we present the first nanomechanical characterization of a cubic phase lipid membrane, through AFM-based Force Spectroscopy (AFM-FS). Our analysis reveals that the mechanical response of these architectures is strictly related to their topology and structure. The observed properties are strikingly similar to those of macroscopic 3D printed cubic structures when subjected to compression tests in material science; suggesting that this behaviour depends on the 3D organisation, rather than on the length-scale of the architecture. We also show for the first time that AFM-FS can be used for characterizing the structure of non-lamellar mesophases, obtaining lattice parameters in agreement with SAXS data. In contrast to classical rheological studies, which can only probe bulk cubic phase solutions, our AFM-FS analysis allows probing the response of cubic membranes to deformations occurring at length and force scales similar to those found in biological interactions.
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