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- Casmo, Veronica, et al.
(författare)
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Occurrence of Cryptosporidium spp. and Cystoisospora belli among adult patients with diarrhoea in Maputo, Mozambique
- 2018
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Ingår i: Heliyon. - : ELSEVIER SCI LTD. - 2405-8440. ; 4:9
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Tidskriftsartikel (refereegranskat)abstract
- Infections with Cryptosporidium spp. and Cystoisospora belli are important causes of diarrhoea in HIV patients. Nevertheless, information concerning these two parasites is scarce in many African countries, including Mozambique. In this study occurrence of Cryptosporidium spp. and C. belli was investigated by microscopy of stool specimens from 108 adult diarrhoeal patients, most with a confirmed HIV diagnosis. The Cryptosporidium isolates were further characterized by molecular methods. Cryptosporidium and C. belli oocysts were found in 8.3% (9/108), and 25.0% (27/108) of the study participants, respectively. Species identification was possible for all Cryptosporidium isolates with available DNA. The following Cryptosporidium species were detected (number of cases within parentheses): C. parvum (3), C. hominis (3), C. felis (1), and C. hominis/C. parvum (1). Subtyping targeting the gp60 gene revealed two C. hominis isolates with subtype IaA23R3, one C. parvum isolate with IIcA5G3d, and one with IIeAl2G1. In summary the occurrence of C. hominis and anthroponotic subtypes of C. parvum indicates that the main route of Cryptosporidium transmission in the present study population was human to human (direct or via food and water). The high prevalence of C. belli highlights the need for early diagnosis of this parasite, for which a treatment exists.
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| 2. |
- Casmo, Veronica, et al.
(författare)
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Regional Differences in Intestinal Parasitic Infections among Army Recruits in a Southern Mozambique Training Center : A Cross-Sectional Study
- 2023
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Ingår i: Pathogens. - : MDPI. - 2076-0817. ; 12:9
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Tidskriftsartikel (refereegranskat)abstract
- Due to the high prevalence and diversity of clinical manifestations, intestinal parasitic infections (IPIs) represent a public health problem. The objective of the work was to determine the prevalence of IPIs among army recruits at a practice and training center in southern Mozambique. Sociodemographic information was obtained through semi-structured interviews. Single urine and stool samples were collected from 362 recruits. Parasite diagnosis was made by filtration, formaldehyde-ether and Kato-Katz techniques. Positive individuals underwent abdominal ultrasound. Then, descriptive statistics and cross-tabulations were performed, and p-values < 0.05 were considered significant. The prevalence of infection with at least one parasite was 25.1% (95% CI: 20.5-29.6; n = 91). The most common parasites were Entamoeba coli (10.7%; 95% CI: 7.4-13.7; n = 37) and Trichuris trichiura (6.1%; 95% CI: 4.6-9.9; n = 25). Parasitic infection was associated with the origin of the participant (p-value < 0.001), and the province of Sofala had the highest prevalence among the provinces studied (70.6%; 95% CI: 47.0-87.8; 12/17). Since oral fecal transmission occurs for several parasites, routine screening and deworming prior to enrollment at the army training center is recommended to reduce transmission of intestinal parasites among recruits.
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| 3. |
- Lindh, Johan, et al.
(författare)
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Occurrence of intestinal parasites and bacteria, with the emphasis on Giardia intestinalis in children under 5 years old in Magude village, Maputo, Mozambique
- 2024
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Ingår i: Transactions of the Royal Society of Tropical Medicine and Hygiene. - : Oxford University Press. - 0035-9203 .- 1878-3503.
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Background: Intestinal protozoa are a major causative agent of gastrointestinal problems in humans and animals. They are detected worldwide, mainly in developing countries where environmental sanitation is inadequate. Infections can affect people of all ages, but children are particularly vulnerable. Here we investigated intestinal protozoa and bacteria in Mozambique.Methods: We analysed the occurence of Giardia intestinalis and other intestinal parasites in stool samples from 291 children under five years of age, by microscopy. Most of the samples (279) were also analysed by multiplex PCR for the occurrence of intestinal parasites and common bacteria. Positive Giardia samples were further subtyped Results: G. intestinalis was detected in 72.2% (n=210/291) of study participants. Assemblage identification was possible in 45 cases by PCR. We identified assemblages; A and B, and four sub-assemblages.Sub-assemblage AI, which may have zoonotic potential, was identified in 37.8% of the analysed samples,. Enteroaggregative E. coli (EAEC) was detected in 77.8% of the faeces samples analysed. PCR was more sensitive for parasite detection than microscopy. Conclusions: Our results indicated that microscopic diagnosis is likely sufficient for identifying symptomatic intestinal infections.The presence of G. intestinalis AI warrants special attention in future studies, and the attention of the Mozambique public health authority.
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